Selective detection of viable Helicobacter pylori using ethidium monoazide or propidium monoazide in combination with real-time polymerase chain reaction

Microbiology and Immunology

Volumn 55, Issue 12, 2011, Pages 841-846

  Nam, Sehee ^a,c   Kwon, Soonbok ^a   Kim, Min jeong ^a   Chae, Jong Chan ^b   Jae Maeng, Pil ^c   Park, Jong Geun ^d   Lee, Gyu Cheol ^a  

^a K water   (South Korea)

^b Chonbuk National University   (South Korea)

^c Chungnam National University   (South Korea)

^d Daegu University   (South Korea)

Author keywords

Ethidium monoazide; Helicobacter pylori; Propidium monoazide; Real time PCR

Indexed keywords

ETHIDIUM; ETHIDIUM AZIDE; GENOMIC DNA; PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BACTERIAL MEMBRANE; BACTERIUM DETECTION; CELL VIABILITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA DEGRADATION; HELICOBACTER PYLORI; INTERMETHOD COMPARISON; MEMBRANE PERMEABILITY; NONHUMAN; PROCESS OPTIMIZATION; REAL TIME POLYMERASE CHAIN REACTION;

AFFINITY LABELS; AZIDES; CELL MEMBRANE; COLONY COUNT, MICROBIAL; DNA, BACTERIAL; HELICOBACTER PYLORI; MICROBIAL VIABILITY; PERMEABILITY; PROPIDIUM; REAL-TIME POLYMERASE CHAIN REACTION;

HELICOBACTER PYLORI;

EID: 84855245328     PISSN: 03855600     EISSN: 13480421     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2011.00388.x     Document Type: Article

References (37)

1. Covacci A., Telford J.L., Del Gludice G., Parsonnet J., Rappuoli R. (1999) Helicobacter pylori virulence and genetic geography. Science 284: 1328-33.
2. Marshall B.J., Warren J.R. (1984) Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1: 1311-5.
3. Dunn B.E., Roop R.M. 2nd, Sung C.C., Sharma S.A., Perez-Perez G.I., Blaser M.J. (1992) Identification and purification of a cpn60 heat shock protein homolog from Helicobacter pylori. Infect Immun 60: 1946-51.
4. Matysiak-Budnik T., Mégraud F. (2006) Helicobacter pylori infection and gastric cancer. Eur J Cancer 42: 708-16.
5. Parsonnet J., Vandersteen D., Goates J., Sibley R.K., Pritikin J., Chang Y. (1991) Helicobacter pylori infection in intestinal- and diffuse-type gastric adenocarcinomas. J Natl Cancer Inst 83: 640-3.
6. Vale F.F., Vítor J.M. (2010) Transmission pathway of Helicobacter pylori: does food play a role in rural and urban areas? Int J Food Microbiol 138: 1-12.
7. Bellack N.R., Koehoorn M.W., MacNab Y.C., Morshed M.G. (2006) A conceptual model of water's role as a reservoir in Helicobacter pylori transmission: a review of the evidence. Epidemiol Infect 134: 439-49.
8. Fujimura S., Kato S., Kawamura T. (2004) Helicobacter pylori in Japanese river water and its prevalence in Japanese children. Lett Appl Microbiol 38: 517-21.
9. Kikuchi S., Dore M.P. (2005) Epidemiology of Helicobacter pylori infection. Helicobacter 10: 1-4.
10. Horiuchi T., Ohkusa T., Watanabe M., Kobayashi D., Miwa H., Eishi Y. (2001) Helicobacter pylori DNA in drinking water in Japan. Microbiol Immunol 45: 515-9.
11. Hulten K., Han S.W., Enroth H., Klein P.D., Opekun A.R., Gilman R.H., Evans D.G., Engstrand L., Graham D.Y., El-Zaatari F.A. (1996) Helicobacter pylori in the drinking water in Peru. Gastroenterology 110: 1031-5.
12. Mazari-Hiriart M., López-Vidal Y., Calva J.J. (2001) Helicobacter pylori in water systems for human use in Mexico City. Water Sci Tech 43: 93-8.
13. Queralt N., Bartolomé R., Araujo R. (2005) Detection of Helicobacter pylori DNA in human faeces and water with different levels of faecal pollution in the north-east of Spain. J Appl Microbiol 98: 889-95.
14. Sheridan G.E., Masters C.I., Shallcross J.A., MacKey B.M. (1998) Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells. Appl Environ Microbiol 64: 1313-8.
15. Vaitilingom M., Gendre F., Brignon P. (1998) Direct detection of viable bacteria, molds and yeasts by reverse transcriptase PCR in contaminated milk samples after heat treatment. Appl Environ Microbiol 64: 1157-60.
16. Chang B., Sugiyama K., Taguri T., Amemura-Maekawa J., Kura F., Watanabe H. (2009) Specific detection of viable Legionella cells by combined use of photoactivated ethidium monoazide and PCR/real-time PCR. Appl Environ Microbiol 75: 147-53.
17. Hein I., Schneeweiss W., Stanek C., Wagner M. (2007) Ethidium monoazide and propidium monoazide for elimination of unspecific DNA background in quantitative universal real-time PCR. J Microbiol Meth 71: 336-9.
18. Nocker A., Cheung C., Camper A. (2006) Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J Microbiol Meth 67: 310-20.
19. Nogva H.K., Drømtorp S.M., Nissen H., Rudi K. (2003) Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5′-nuclease PCR. Biotechniques 34: 804-13.
20. Rudi K., Moen B., Drømtorp S.M., Holck A.L. (2005) Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples. Appl Environ Microbiol 71: 1018-24.
21. Hixon S.C., White W.E., Yielding K.L. (1975) Selective covalent binding of an ethidium analog to mitochondrial DNA with production of petite mutants in yeast by photoaffinity labeling. J Mol Biol 92: 319-29.
22. Waring M.J. (1965) Complex formation between ethidium bromide and nucleic acids. J Mol Biol 13: 269-82.
23. Nocker A., Sossa K.E., Camper A.K. (2007) Molecular monitoring of disinfection efiicacy using propidium monoazide in combination with quantitative PCR. J Microbiol Meth 70: 252-60.
24. Spiegelhalder C., Gerstenecker B., Kersten A. (1993) Purification of Helicobacter pylori superoxide dismutase and cloning sequencing of the gene. Infect Immun 61: 5315-25.
25. Bae S., Wuertz S. (2009) Rapid decay of host-specific fecal Bacteroidales cells in seawater as measured by quantitative PCR with propidium monoazide. Water Res 43: 4850-9.
26. Brescia C.C., Griffin S.M., Ware M.W., Varughese E.A., Egorov A.I., Villegas E.N. (2009) Cryptosporidium propidium monoazide-PCR, a molecular biology-based technique for genotyping of viable Cryptosporidium oocysts. Appl Environ Microbiol 75: 6856-63.
27. Lee J.L., Levin R.E. (2009) A comparative study of the ability of EMA and PMA to distinguish viable from heat killed mixed bacterial flora from fish fillets. J Microbiol Meth 76: 93-6.
28. Rawsthorne H., Dock C.N., Jaykus L.A. (2009) PCR-based method using propidium monoazide to distinguish viable from nonviable Bacillus subtilis spores. Appl Environ Microbiol 75: 2936-9.
29. Agustí, G., Codony, F., Fittipaldi, M., Adrados, B., Morató, J. (2010) Viability determination of Helicobacter pylori using propidium monoazide quantitative PCR. Helicobacter 15: 473-6.
30. Wang S., Levin R.E. (2006) Discrimination of viable Vibrio vulnificus cells from dead cells in real-time PCR. J Microbiol Meth 64: 1-8.
31. Andersen L.P., Rasmussen L. (2009) Helicobacter pylori-coccoid forms and biofilm formation. FEMS Immunol Med Microbiol 56: 112-5.
32. Cellini L., Allocati N., Angelucci D., Iezzi T., Di Campli E., Marzio L., Dainelli B. (1994) Coccoid Helicobacter pylori not culturable in vitro reverts in mice. Microbiol Immunol 38: 843-50.
33. Willén R., Carlén B., Wang X., Papadogiannakis N., Odselius R., Wadström T. (2000) Morphologic conversion of Helicobacter pylori from spiral to coccoid form. Scanning (SEM) and transmission electron microscopy (TEM) suggest viability. Upsala J Med Sci 105: 31-40.
34. Azevedo N.F., Guimaraes N., Figueiredo C., Keevil C.W., Vieira M.J. (2007) A new model for the transmission of Helicobacter pylori: role of environmental reservoirs as gene pools to increase strain diversity. Crit Rev Microbiol 33: 157-69.
35. Giao M.S., Azevedo N.F., Wilks S.A., Vieira M.J., Keevil C.W. (2008) Persistence of Helicobacter pylori in heterotrophic drinking-water biofilms. Appl Environ Microbiol 74: 5898-904.
36. Park S.R., MacKay W.G., Reid D.C. (2001) Helicobacter sp. recovered from drinking water biofilm sampled from a water distribution system. Water Res 35: 1624-26.
37. Percival S.L., Thomas J.G. (2009) Transmission of Helicobacter pylori and the role of water and biofilms. J Water Health 7: 469-77.