Primer evaluation and adaption for cost-efficient SYBR Green-based qPCR and its applicability for specific quantification of methanogens

World Journal of Microbiology and Biotechnology

Volumn 30, Issue 1, 2014, Pages 293-304

  Reitschuler, Christoph ^a   Lins, Philipp ^a   Illmer, Paul ^a  

^a UNIVERSITY OF INNSBRUCK   (Austria)

Author keywords

Methanogens; Primer; qPCR; Specificity; SYBR Green

Indexed keywords

ACCURATE QUANTIFICATIONS; DENATURING GRADIENT GEL; METHANOGENIC COMMUNITY; METHANOMICROBIALES (MMB); PRIMER; QPCR; SPECIFICITY; SYBR GREEN;

POLYMERASE CHAIN REACTION; PROBES; RNA;

METHANOGENS;

ARCHAEA; METHANOBACTERIALES; METHANOCOCCALES; METHANOCULLEUS; METHANOMICROBIALES; METHANOSAETA; METHANOSARCINA; METHANOSARCINALES; METHANOTHERMOBACTER;

ORGANIC COMPOUND; PRIMER DNA;

ARCHAEON; ARTICLE; CELL COUNT; CLASSIFICATION; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SYBR GREEN I;

ARCHAEA; CELL COUNT; DNA PRIMERS; MICROBIOLOGICAL TECHNIQUES; ORGANIC CHEMICALS; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 84891660302     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-013-1450-x     Document Type: Article

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