Design and testing of real-time PCR primers for the quantification of Methanoculleus, Methanosarcina, Methanothermobacter, and a group of uncultured methanogens

Canadian Journal of Microbiology

Volumn 55, Issue 5, 2009, Pages 611-616

  Franke Whittle, Ingrid H ^a   Goberna, Marta ^a   Insam, Heribert ^a  

^a UNIVERSITY OF INNSBRUCK   (Austria)

Author keywords

16S rRNA gene; Biogas; Methanogens; Real time PCR; SYBR Green

Indexed keywords

16S RRNA GENE; ANAEROBIC HABITATS; ARCHAEA; BIOGAS PRODUCTION; END POINTS; PCR PRIMERS; REAL-TIME PCR; REAL-TIME PCR ASSAY; SYBR GREEN;

BIOGAS; METHANOGENS;

ANAEROBIC DIGESTION;

BIOGAS; CYANINE DYE; RNA 16S;

DESIGN; METHANOGENIC BACTERIUM; REAL TIME;

ANAEROBIC DIGESTION; ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; GENE SEQUENCE; GENUS; METHANOCULLEUS; METHANOGEN; METHANOSARCINA; METHANOTHERMOBACTER; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION;

DNA PRIMERS; METHANOBACTERIACEAE; METHANOMICROBIACEAE; METHANOSARCINA; POLYMERASE CHAIN REACTION; RNA, ARCHAEAL; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY;

METHANOCULLEUS; METHANOSAETA; METHANOSARCINA; METHANOTHERMOBACTER; UNCULTURED METHANOGENIC ARCHAEON;

EID: 66849138429     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W08-157     Document Type: Article

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