Cloning and functional analysis of the Gβ gene Mgb1 and the Gγ gene Mgg1 in Monascus ruber

Journal of Microbiology

Volumn 52, Issue 1, 2014, Pages 35-43

  Li, Li ^a,b   He, Lu ^a   Lai, Yong ^a   Shao, Yanchun ^a   Chen, Fusheng ^a  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

^b YANGTZE UNIVERSITY   (China)

Author keywords

citrinin; G protein beta gamma; growth; Monascus ruber; pigment; reproduction

Indexed keywords

FUNGAL DNA; GUANINE NUCLEOTIDE BINDING PROTEIN;

ARTICLE; ASPERGILLUS; CHEMISTRY; DNA SEQUENCE; ENZYMOLOGY; GENE DELETION; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MONASCUS; PROTEIN SUBUNIT; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION;

ASPERGILLUS; CLONING, MOLECULAR; DNA, FUNGAL; GENE DELETION; GTP-BINDING PROTEINS; MOLECULAR SEQUENCE DATA; MONASCUS; PROTEIN SUBUNITS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SIGNAL TRANSDUCTION;

EID: 84891615940     PISSN: 12258873     EISSN: 19763794     Source Type: Journal    
DOI: 10.1007/s12275-014-3072-x     Document Type: Article

References (49)

1. Antal, Z., Rascle, C., Fevre, M., and Bruel, C. 2004. Single oligonucleotide nested PCR: a rapid method for the isolation of genes and their flanking regions from expressed sequence tags. Curr. Genet. 46, 240-246.
2. Blanc, P. J., Laussac, J. P., Lebars, J., Lebars, P., Loret, M. O., Pareilleux, A., Prome, D., Prome, J. C., Santerre, A. L., and Goma, G. 1995a. Characterization of monascidin-A from Monascus as citrinin. Int. J. Food Microbiol. 27, 201-213.
3. Blanc, P. J., Loret, M. O., and Goma, G. 1995b. Production of citrinin by various species of Monascus. Biotechnol. Lett. 17, 291-294.
4. Chang, M. H., Chae, K. S., Han, D. M., and Jahng, K. Y. 2004. The GanB Gα-protein negatively regulates asexual sporulation and plays a positive role in conidial germination in Aspergillus nidulans. Genetics167, 1305-1315.
5. Delgado-Jarana, J., Martinez-Rocha, A. L., Roldan-Rodriguez, R., Roncero, M. I., and Di Pietro, A. 2005. Fusarium oxysporum G protein β subunit Fgb1 regulates hyphal growth, development, and virulence through multiple signalling pathways. Fungal Genet. Biol. 42, 61-72.
6. Fan, Y. Y. 2007. Cloning and characterization of a G-protein Γ subunit gene in the chestnut blight fungus, Cryphonectria parasitica. Master's theses, Guangxi University, Nanning, Guangxi, P. R. China.
7. Feng, Y., Shao, Y., and Chen, F. 2013. Monascus pigments. Appl. Microbiol. Biotechnol. 96, 1421-1440.
8. Ganem, S., Lu, S. W., Lee, B. N., Chou, D. Y., Hadar, R., Turgeon, B. G., and Horwitz, B. A. 2004. G-protein β subunit of Cochliobolus heterostrophus involved in virulence, asexual and sexual reproductive ability, and morphogenesis. Eukaryot. Cell3, 1653-1663.
9. Han, K. H., Seo, J. A., and Yu, J. H. 2004. Regulators of G-protein signalling in Aspergillus nidulans: RgsA downregulates stress response and stimulates asexual sporulation through attenuation of GanB (Gα) signalling. Mol. Microbiol. 53, 529-540.
10. Hao, Z., Li, L., Li, Z., Han, J., Lu, Q., Tong, W., and Dong J. 2010. Construction of sense and antisense expression vectors and preliminary functional research of Stgb-1 gene in Setosphaeria turcica (Chinese). J. Agricultural University of Hebei33, 1-6.
11. He, Y., Liu, Q., Shao, Y., and Chen, F. 2013. ku70 and ku80 null mutants improve the gene targeting frequency in Monascus ruber M7. Appl. Microbiol. Biotechnol. 97, 4965-4976.
12. Hsu, W. H. and Pan, T. M. 2012. Monascus purpureus-fermented products and oral cancer: a review. Appl. Microbiol. Biotechnol. 93, 1831-1842.
13. Jain, S., Akiyama, K., Kan, T., Ohguchi, T., and Takata, R. 2003. The G protein β subunit FGB1 regulates development and pathogenicity in Fusarium oxysporum. Curr. Genet. 43, 79-86.
14. Jia, X. Q., Xu, Z. N., Zhou, L. P., and Sung, C. K. 2010. Elimination of the mycotoxin citrinin production in the industrial important strain Monascus purpureus SM001. Metab. Eng. 12, 1-7.
15. Kasahara, S. and Nuss, D. L. 1997. Targeted disruption of a fungal G-protein β subunit gene results in increased vegetative growth but reduced virulence. Mol. Plant Microbe Interact. 10, 984-993.
16. Krystofova, S. and Borkovich, K. A. 2005. The heterotrimeric G-protein subunits GNG-1 and GNB-1 form a GβΓ dimer required for normal female fertility, asexual development, and Gα protein levels in Neurospora crassa. Eukaryot. Cell 4, 365-378.
17. Lafon, A., Seo, J. A., Han, K. H., Yu, J. H., and d'Enfert, C. 2005. The heterotrimeric G-protein GanB(α)-SfaD(β)-GpgA(Γ) is a carbon source sensor involved in early cAMP-dependent germination in Aspergillus nidulans. Genetics 171, 71-80.
18. Lee, C. L., Hung, H. K., Wang, J. J., and Pan, T. M. 2007. Improving the ratio of monacolin K to citrinin production of Monascus purpureus NTU 568 under dioscorea medium through the mediation of pH value and ethanol addition. J. Agr. Food Chem. 55, 6493-6502.
19. Lee, C. L. and Pan, T. M. 2011. Red mold fermented products and Alzheimer's disease: a review. Appl. Microbiol. Biotechnol. 91, 461-469.
20. Lee, B. H. and Pan, T. M. 2012. Benefit of Monascus-fermented products for hypertension prevention: a review. Appl. Microbiol. Biotechnol. 94, 1151-1161.
21. Li, L., Shao, Y., Li, Q., Yang, S., and Chen, F. 2010. Identification of Mga1, a G-protein α-subunit gene involved in regulating citrinin and pigment production in Monascus ruber M7. FEMS Microbiol. Lett. 308, 108-114.
22. Li, L., Wright, S. J., Krystofova, S., Park, G., and Borkovich, K. A. 2007. Heterotrimeric G protein signaling in filamentous fungi. Annu. Rev. Microbiol. 61, 423-452.
23. Liang, S., Wang, Z. Y., Liu, P. J., and Li, D. B. 2006. A GΓ subunit promoter T-DNA insertion mutant A1-412 of Magnaporthe grisea is defective in appressorium formation, penetration and pathogenicity (Chinese). Chinese Science Bulletin51, 2037-2040.
24. Maria Laxalt, A., Latijnhouwers, M., van Hulten, M., and Govers, F. 2002. Differential expression of G protein α and β subunit genes during development of Phytophthora infestans. Fungal Genet. Biol. 36, 137-146.
25. Mehrabi, R., Ben M'Barek, S., van der Lee, T. A., Waalwijk, C., de Wit, P. J., and Kema, G. H. 2009. Gα and Gβ proteins regulate the cyclic AMP pathway that is required for development and pathogenicity of the phytopathogen Mycosphaerella graminicola. Eukaryot. Cell 8, 1001-1013.
26. Muller, P., Leibbrandt, A., Teunissen, H., Cubasch, S., Aichinger, C., and Kahmann, R. 2004. The Gβ-subunit-encoding gene bpp1 controls cAMP signaling in Ustilago maydis. Eukaryot. Cell3, 806-814.
27. Nishimura, M., Park, G., and Xu, J. R. 2003. The G-beta subunit MGB1 is involved in regulating multiple steps of infection-related morphogenesis in Magnaporthe grisea. Mol. Microbiol. 50, 231-243.
28. Osada, S., Hosaka, T., Nakajima, M., and Akutsu, K. 2005. Molecular cloning of a novel Gα subunit gene and a Gβ subunit gene from the gray mold fungus Botrytis cinerea. J. Gen. Plant Pathol. 71, 408-413.
29. Palmer, D. A., Thompson, J. K., Li, L., Prat, A., and Wang, P. 2006. Gib2, a novel Gβ-like/RACK1 homolog, functions as a Gβ subunit in cAMP signaling and is essential in Cryptococcus neoformans. J. Biol. Chem. 281, 32596-32605.
30. Parsley, T. B., Segers, G. C., Nuss, D. L., and Dawe, A. L. 2003. Analysis of altered G-protein subunit accumulation in Cryphonectria parasitica reveals a third Gα homologue. Curr. Genet. 43, 24-33.
31. Patakova, P. 2013. Monascus secondary metabolites: production and biological activity. J. Ind. Microbiol. Biotechnol. 40, 169-181.
32. Pattanagul, P., Pinthong, R., Phianmongkhol, A., and Tharatha, S. 2008. Mevinolin, citrinin and pigments of adlay angkak fermented by Monascus sp. Int. J. Food Microbiol. 126, 20-23.
33. Rosen, S., Yu, J. H., and Adams, T. H. 1999. The Aspergillus nidulans sfaD gene encodes a G protein β subunit that is required for normal growth and repression of sporulation. EMBO J. 18, 5592-5600.
34. Sagaram, U. S. and Shim, W. B. 2007. Fusarium verticillioides GBB1, a gene encoding heterotrimeric G protein β subunit, is associated with fumonisin B biosynthesis and hyphal development but not with fungal virulence. Mol. Plant Pathol. 8, 375-384.
35. Seo, J. A., Han, K. H., and Yu, J. H. 2005. Multiple roles of a heterotrimeric G-protein Γ-subunit in governing growth and development of Aspergillus nidulans. Genetics 171, 81-89.
36. Seo, J. A. and Yu, J. H. 2006. The phosducin-like protein PhnA is required for GβΓ-mediated signaling for vegetative growth, developmental control, and toxin biosynthesis in Aspergillus nidulans. Eukaryot. Cell5, 400-410.
37. Servin, J. A., Campbell, A. J., and Borkovich, K. A. 2012. G protein signaling components in filamentous fungal genomes. In Witzany, G. (eds), Biocommunication of Fungi, pp. 21-38. Springer, Netherlands.
38. Shi, Y. C. and Pan, T. M. 2012. Red mold, diabetes, and oxidative stress: a review. Appl. Microbiol. Biotechnol. 94, 47-55.
39. Shin, K. S., Kwon, N. J., and Yu, J. H. 2009. G βΓ-mediated growth and developmental control in Aspergillus fumigatus. Curr. Genet. 55, 631-641.
40. van Tieghem, M. 1884. Monascus genre nouvear de l'ondre des Ascomycetes. Bull Soc. Bot. Fr. 31, 226-231.
41. Wang, Y. Z., Ju, X. L., and Zhou, Y. G. 2005. The variability of citrinin production in Monascus type cultures. Food Microbiol. 22, 145-148.
42. Wang, P., Perfect, J. R., and Heitman, J. 2000. The G-protein β subunit GPB1 is required for mating and haploid fruiting in Cryptococcus neoformans. Mol. Cell. Biol. 20, 352-362.
43. Wu, J. P. 2009. Cloning and functional analysis of genes encoding heterotrimeric G protein gamma subunit in Setosphaeria turcica. Master's theses of Agricultural University of Hebei, Baoding, Hebei, P. R. China.
44. Yang, Y., Li, L., Li, X., Shao, Y., and Chen, F. 2012. mrflbA, encoding a putative FlbA, is involved in aerial hyphal development and secondary metabolite production in Monascus ruber M7. Fungal Biol. 116, 225-233.
45. Yang, Q., Poole, S. I., and Borkovich, K. A. 2002. A G-protein β subunit required for sexual and vegetative development and maintenance of normal Gα protein levels in Neurospora crassa. Eukaryot. Cell 1, 378-390.
46. Yu, J. H. 2006. Heterotrimeric G protein signaling and RGSs in Aspergillus nidulans. J. Microbiol. 44, 145-154.
47. Yu, J. H., Hamari, Z., Han, K. H., Seo, J. A., Reyes-Dominguez, Y., and Scazzocchio, C. 2004. Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. Fungal Genet. Biol. 41, 973-981.
48. Yu, H. Y., Seo, J. A., Kim, J. E., Han, K. H., Shim, W. B., Yun, S. H., and Lee, Y. W. 2008. Functional analyses of heterotrimeric G protein Gα and Gβ subunits in Gibberella zeae. Microbiology 154, 392-401.
49. Yu, J. H., Wieser, J., and Adams, T. H. 1996. The Aspergillus FlbA RGS domain protein antagonizes G protein signaling to block proliferation and allow development. EMBO J. 15, 5184-5190.