Analysis of altered G-protein subunit accumulation in Cryphonectria parasitica reveals a third Gα homologue

Current Genetics

Volumn 43, Issue 1, 2003, Pages 24-33

  Parsley, Todd B ^a,b   Segers, Gert C ^a   Nuss, Donald L ^a   Dawe, Angus L ^a  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b TherImmune Research Corporation   (United States)

Author keywords

G protein alpha subunit; Protein accumulation; Protein turnover

Indexed keywords

BUFFER; FUNGAL PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN; HETEROTRIMERIC GUANINE NUCLEOTIDE BINDING PROTEIN; MONOCLONAL ANTIBODY; PROTEIN SUBUNIT;

ALPHA CHAIN; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CROSS REACTION; CRYPHONECTRIA PARASITICA; CULTURE MEDIUM; DILUTION; EUKARYOTIC CELL; EXTRACTION; FUNGAL STRAIN; FUNGUS; FUNGUS MUTANT; GENE DELETION; GENE DISRUPTION; GENE SEQUENCE; IMMUNOBLOTTING; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PHYLOGENY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN INTERACTION; SEQUENCE HOMOLOGY; STIMULUS RESPONSE; TURNOVER TIME;

ANIMALIA; CRYPHONECTRIA; CRYPHONECTRIA PARASITICA; EUKARYOTA; FUNGI; TEREBRANTIA;

EID: 0038605751     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-003-0367-6     Document Type: Article

References (33)

1. Aimi T, Kano S, Wang Q, Morinaga T (2001) Molecular cloning of three genes encoding G-protein alpha subunits in the white root rot fungus, Rosellinia necatrix. Biosci Biotechnol Biochem 65:678-682
2. Bölker M (1998) Sex and crime: heterotrimeric G proteins in fungal mating and pathogenesis. Fungal Genet Biol 25:143-156
3. Bowman EJ, Bowman BJ (1988) Purification of vacuolar membranes, mitochondria, and plasma membranes from Neurospora crassa and modes of discriminating among the different H+-ATPases. Methods Enzymol 157:562-573
4. Chen CA, Manning DR (2001) Regulation of G proteins by covalent modification. Oncogene 20:1643-1652
5. Choi GH, Larson TG, Nuss DL (1992) Molecular analysis of the laccase gene from the chestnut blight fungus and selective suppression of its expression in an isogenic hypovirulent strain. Mol Plant Microbe Interact 5:119-128
6. Choi GH, Chen B, Nuss DL (1995) Virus-mediated or transgenic suppression of a G-protein alpha subunit and attenuation of fungal virulence. Proc Natl Acad Sci USA 92:305-309
7. Churchill ACL, Ciufetti LM, Hansen DR, Van Etten HD, Van Alfen NK (1990) Transformation of the fungal pathogen Cryphonectria parasitica with a variety of heterologous plasmids. Curr Genet 17:25-31
8. Dawe AL, Nuss DL (2001) Hypoviruses and chestnut blight: exploiting viruses to understand and modulate fungal pathogenesis. Annu Rev Genet 35:1-29
9. Dohlman HG, Song J, Ma D, Courchesne WE, Thorner J (1996) Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein a subunit). Mol Cell Biol 16:5194-5209
10. Gao S, Nuss DL (1996) Distinct roles for two G protein a subunits in fungal virulence, morphology, and reproduction revealed by targeted gene disruption. Proc Natl Acad Sci USA 93:14122-14127
11. Gao S, Nuss DL (1998) Mutagenesis of putative acylation sites alters function, localization, and accumulation of a Gi alpha subunit of the chestnut blight fungus Cryphonectria parasitica. Mol Plant Microbe Interact 11: 1130-1135
12. Gronover CS, Kasulke D, Tudzynski P, Tudzynski B (2001) The role of G-protein alpha subunits in the infection process of the gray mold fungus Botrytis cinerea. Mol Plant Microbe Interact 14:1293-1302
13. Hamm HE (1998) The many faces of G protein signaling. J Biol Chem 273:669-672
14. Higgins JB, Casey PJ (1996) The role of prenylation in G-protein assembly and function. Cell Signal 8:433-437
15. Ivey FD, Yang Q, Borkovich KA (1999) Positive regulation of adenylyl cyclase activity by a Gai homolog in Neurospora crassa. Fungal Genet Biol 26:48-61
16. Kasahara S, Nuss DL (1997) Targeted disruption of a fungal G-protein beta subunit gene results in increased vegetative growth but reduced virulence. Mol Plant Microbe Interact 10:984-993
17. Kasahara S, Wang P, Nuss DL (2000) Identification of bdm-1, a gene involved in G protein beta-subunit function and alpha-subunit accumulation. Proc Natl Acad Sci USA 97:412-417
18. Linder ME, Middleton P, Hepler JR, Taussig R, Gilman AG, Mumby SM (1993) Lipid modifications of G proteins: alpha subunits are palmitoylated. Proc Natl Acad Sci USA 90:3675-3679
19. Liu S, Dean RA (1997) G protein alpha subunit genes control growth, development, and pathogenicity of Magnaporthe grisea. Mol Plant Microbe Interact 10:1075-1086
20. Loubradou G, Begueret J, Turcq B (1999) MOD-D, a G-a subunit of the fungus Podospora anserina, is involved in both regulation of development and vegetative incompatibility. Genetics 152:519-528
21. Manahan CL, Patnana M, Blumer KJ, Linder ME (2000) Dual lipid modification motifs in Ga and Gg subunits are required for full activity of the pheromone response pathway in Saccharomyces cerevisiae. Mol Biol Cell 11:957-968
22. Mumby SM, Kleuss C, Gilman AG (1994) Receptor regulation of G-protein palmitoylation. Proc Natl Acad Sci USA 91:2800-2804
23. Orbach MJ, Porro EB, Yanofsky C (1986) Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Mol Cell Biol 6:2452-2461
24. Parker EM, Kameyama K, Higashijima T, Ross EM (1991) Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cells. J Biol Chem 266:519-527
25. Parsley TB, Chen B, Geletka LM, Nuss DL (2002) Differential modulation of cellular signaling pathways by mild and severe hypovirus strains. Eukaryot Cell 1:401-413
26. Puhalla JE, Anagnostakis SL (1971) Genetics and nutritional requirements of Endothia parasitica. Phytopathology 61:169-173
27. Rudolph U, Spicher K, Birnbaumer L (1996) Adenylyl cyclase inhibition and altered G protein subunit expression and ADP-ribosylation patterns in tissues and cells from Gi2a -/- mice. Proc Natl Acad Sci USA 93:3209-3214
28. Sankaram MB, Marsh D (1993) Protein-lipid interactions with peripheral membrane proteins. In: Watts AD (ed) Protein and lipid interactions. Elsevier, Amsterdam, pp 127-162
29. Segers GC, Nuss DL (2002) Constitutively activated Ga negatively regulates virulence, reproduction and hydrophobin gene expression in the chestnut blight fungus Cryphonectria parasitica. Fungal Genet Biol (in press)
30. Song J, Hirschman J, Gunn K, Dohlman HG (1996) Regulation of membrane and subunit interactions by N-myristoylation of a G protein a subunit in yeast. J Biol Chem 271:20273-20283
31. Turner GE, Borkovich KA (1993) Identification of a G protein alpha subunit from Neurospora crassa that is a member of the Gi family. J Biol Chem 268:14805-14811
32. Wedegaertner PB, Wilson PT, Bourne HR (1995) Lipid modifications of trimeric G proteins. J Biol Chem 270:503-506
33. Yang Q, Poole SI, Borkovich KA (2002) A G-protein beta subunit required for sexual and vegetative development and maintenance of normal Ga protein levels in Neurospora crassa. Eukaryot Cell 1:378-390