Increased expression of clp genes in Lactobacillus delbrueckii UFV H2b20 exposed to acid stress and bile salts

Beneficial Microbes

Volumn 4, Issue 4, 2013, Pages 367-374

  Ferreira, A B ^a   De Oliveira, M N V ^b   Freitas, F S ^a   Alfenas Zerbini, P ^a   Da Silva, D F ^c   De Queiroz, M V ^a   Borges, A C ^a   De Moraes, C A ^a  

^a FEDERAL UNIVERSITY OF VIÇOSA   (Brazil)

^b FEDERAL UNIVERSITY OF JUIZ DE FORA   (Brazil)

^c UNIVERSIDADE FEDERAL DOS VALES DO JEQUITINHONHA E MUCURI   (Brazil)

Author keywords

Molecular phylogeny; Probiotics; Quantitative real time PCR; Stress response

Indexed keywords

BILE SALT; PROBIOTIC AGENT;

ACIDITY; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL SURVIVAL; CLPE GENE; CLPL GENE; CLPP GENE; CLPX GENE; CONTROLLED STUDY; EXPOSURE; GASTROINTESTINAL TRACT; GENE EXPRESSION; GENETIC IDENTIFICATION; LACTOBACILLUS DELBRUECKII; MOLECULAR PHYLOGENY; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENETIC TREE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS;

MOLECULAR PHYLOGENY; PROBIOTICS; QUANTITATIVE REAL-TIME PCR; STRESS RESPONSE;

ACIDS; BILE ACIDS AND SALTS; ENDOPEPTIDASE CLP; GENE EXPRESSION PROFILING; HEAT-SHOCK PROTEINS; HYDROGEN-ION CONCENTRATION; LACTOBACILLUS DELBRUECKII; REAL-TIME POLYMERASE CHAIN REACTION; STRESS, PHYSIOLOGICAL; TIME FACTORS;

EID: 84891596353     PISSN: 18762883     EISSN: 18762891     Source Type: Journal    
DOI: 10.3920/BM2013.0022     Document Type: Article

References (29)

1. Altermann, E., Russell, W.M., Azcarate-Peril, M.A., Barrangou, R.,Buck, B.L., McAuliffe, O., Dobson, A., Callanan, M., Lick, S.,Hamrick, A., Cano, R. and Klaenhammer, T.R., 2005. Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM. Proceedings of the National Academy of Sciences of the USA 102: 3906-3912.
2. Altschul, S.F., Gish, W., Miller, W., Myers, E.W. and Lipman, D.J., 1990.Basic local alignment search tool. Journal of Molecular Biology 215: 403-410.
3. Begley, M., Gahan, C.G.M. and Hill, C., 2005. The interaction between bacteria and bile. FEMS Microbiology Reviews 29: 625-651.
4. Berger, B., Pridmore, R.D., Barretto, C., Delmas-Julien, F., Schreiber,K., Arigoni, F. and Brussow, H., 2007. Similarity and differences in the Lactobacillus acidophilus group identified by polyphasic analysis and comparative genomics. Journal of Bacteriology 189: 1311-1321.
5. Conceição, L.L., Leandro, E.S., Freitas, F.S., Oliveira, M.N.V., Ferreira-Machado, A.B., Borges, A.C. and Moraes, C.A., 2013. Survival of Lactobacillus delbrueckii UFV H2b20 in fermented milk under simulated gastric and intestinal conditions. Beneficial Microbes 4: 285-289.
6. Do Carmo, A.P., De Oliveira, M.N.V., Da Silva, D.F., Castro, S.B.,Borges, A.C., De Carvalho, A.F. and De Moraes, C.A., 2012. Genes involved in lactose catabolism and organic acid production during growth of Lactobacillus delbrueckii UFV H2b20 in skimmed milk. Beneficial Microbes 3: 23-32.
7. Felis, G.E. and Dellaglio, F., 2005. Taxonomy of lactobacilli and bifidobacteria. Current Issues in Intestinal Microbiology 8: 44-61.
8. Floresta, F.A., 2008. Características de superfície de Lactobacillus delbrueckii UFV H2b20 probiótico. PhD thesis. Universidade Federal de Viçosa, Viçosa, Brazil.
9. Frees, D., Savijoki, K., Varmanen, P. and Ingmer, H., 2007. Clp ATPases and ClpP proteolytic complexes regulate vital biological processes in low GC, Gram-positive bacteria. Molecular Microbiology 63:1285-1295.
10. Goh, Y.J. and Klaenhammer, T.R., 2009. Genomic features of Lactobacillus species. Frontiers in Bioscience 14: 1362-1386.
11. Larkin, M., Blackshields, G., Brown, N.P., Chenna, R., McGettigan,P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez,R., Thompson, J.D., Gibson, T.J. and Higgins, D.G., 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947-2948.
12. Len, A.C.L., Harty, D.W.S. and Jacques, N.A., 2004. Stress-responsive proteins are upregulated in Streptococcus mutans during acid tolerance. Microbiology 150: 1339-1351.
13. Livak, K.J. and Schimittgen, T.D., 2001. Analysis of relative gene expression data using real time quantitative PCR and the 2-??Ct method. Methods 25: 402-408.
14. Magalhães, J.T., Uetanabaro, A.P.T. and Moraes, C.A., 2008.Identification of Lactobacillus delbrueckii UFV H2b20 (probiotic strain) using DNA-DNA hybridization. Brazilian Journal of Microbiology 39: 524-546.
15. Pieterse, B., Leer, R.J., Schuren, F.H. and Van der Werf, M.J., 2005.Unravelling the multiple effects of lactic acid stress on Lactobacillus plantarum by transcription profiling. Microbiology 151: 3881-3894.
16. Posada, D. and Buckley, T.R., 2004. Model selection and model averaging in phylogenetics: advantages of Akaike information criterion and Bayesian approaches over likelihood ratio tests. Systematic Biology 53: 793-808.
17. Ronquist, F. and Huelsenbeck, J.P., 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572-1574.
18. Sabioni, N.S.S., Pinheiro, A.J.R. and Teixeira, M.A., 1988. Acidophilus milk: isolation and characterization of Lactobacillus acidophillus from breast-fed children and from young calves feces. Review Microbiology 19: 393-398.
19. Sambrook, J., Fritsch, E.F. and Maniatis, T., 1989. Molecular cloning a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Long Island, NY, USA
20. Santos, N.S., 1984. Isolamento e caracterização de Lactobacillus acidophilus de fezes de crianças alimentadas ao seio e de bezerros,visando sua utilização como adjunto dietético. PhD thesis,Universidade Federal de Viçosa, Viçosa, Brazil.
21. Schleifer, K.H. and Ludwig, W., 1995. Phylogeny of the genus Lactobacillus and related genera. Systematic and Applied Microbiology 14: 461-467.
22. Serrazanetti, D.I., Guerzoni, M.E., Corsetti, A. and Vogel, R., 2009.Metabolic impact and potential exploitation of the stress reactions in lactobacilli. Food Microbiology 26: 700-711.
23. Suokko, A., Savijoki, K., Malinen, E., Palva, A. and Varmanen, P.,2005. Characterization of a mobile clpL gene from Lactobacillus rhamnosus. Applied and Environmental Microbiology 71: 2061-2069.
24. Van de Guchte, A.M., Penaud, S., Grimaldi, C., Barbe, V., Bryson, K.,Nicolas, P., Robert, C., Oztas, S., Mangenot, S., Couloux, A., Loux,V., Dervyn, R., Bossy, R., Bolotin, A., Batto, J.M., Walunas, T., Gibrat,J.F., Bessieres, P., Weissenbach, J., Ehrlich, S.D. and Maguin, E.,2006. The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution. Proceedings of the National Academy of Sciences of the USA 103: 9275-9279.
25. Van de Gutche, A.M., Serror, P., Chervaux, C., Smokvina, T., Ehrlich,S.D. and Maguin, E., 2002. Stress response in lactic acid bacteria. Antonie van Leeuwenhoek 82: 187-216.
26. Wall, T., Bath, K., Britton, R.A., Jonsson, H., Versalovic, J. and Ross,S., 2007. The early response to acid shock in Lactobacillus reuteri involves the ClpL chaperone and a putative cell wall-altering esterase. Applied and Environmental Microbiology 73: 3924-3935.
27. Wang, J., Hartling, J.A. and Flanagan, J.M., 1997. The structure of ClpP at 2.3A resolution suggests a model for ATP-dependent proteolysis. Cell 91: 447-456.
28. Whitehead, K., Versalovic, J., Roos, S. and Britton, R.A., 2008. Genomic and genetic characterization of the bile stress response of probiotic Lactobacillus reuteri ATCC 55730. Applied and Environmental Microbiology 74: 1812-1819.
29. Zhu, Y., Zhang, Y. and Li, Y., 2009. Understanding the industrial application potential of lactic acid bacteria through genomics. Applied Microbiology and Biotechnology 83: 597-610.