Pathogen detection by core-shell type aptamer-magnetic preconcentration coupled to real-time PCR

Analytical Biochemistry

Volumn 447, Issue 1, 2014, Pages 119-125

  Ozalp, V Cengiz ^a   Bayramoglu, Gulay ^b   Kavruk, Murat ^c,d   Keskin, Batuhan B ^c   Oktem, Huseyin A ^c   Arica, M Yakup ^b  

^a ALTINBAS UNIVERSITY   (Turkey)

^b GAZI UNIVERSITY   (Turkey)

^c MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

^d Necatibey Cad No: 112   (Turkey)

Author keywords

Aptamers; Biosensors; Magnetic beads; Pathogens; rtPCR

Indexed keywords

BIOSENSORS; ESCHERICHIA COLI; FOOD SUPPLY; HEALTH RISKS; MAGNETIC SEPARATION; PATHOGENS; SALMONELLA; SUPPLY CHAINS;

APTAMERS; BACTERIAL CONTAMINATION; MAGNETIC BEADS; MAGNETIC SEPARATION SYSTEMS; QUANTITATIVE POLYMERASE CHAIN REACTION; RTPCR; SAMPLE PREPARATION METHODS; SUSPENSION POLYMERIZATION;

POLYMERASE CHAIN REACTION;

APTAMER;

ARTICLE; BACTERIUM CONTAMINATION; BACTERIUM DETECTION; BINDING AFFINITY; ESCHERICHIA COLI; FOOD CONTAMINATION; MAGNETIC FIELD; MAGNETIC SEPARATION; MILK; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA TYPHIMURIUM;

APTAMERS; BIOSENSORS; MAGNETIC BEADS; PATHOGENS; RTPCR;

ANALYTIC SAMPLE PREPARATION METHODS; APTAMERS, NUCLEOTIDE; ESCHERICHIA COLI O157; FOOD MICROBIOLOGY; MAGNETIC PHENOMENA; MICROSPHERES; POLYMERS; REAL-TIME POLYMERASE CHAIN REACTION; SALMONELLA TYPHI;

EID: 84891544224     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.11.022     Document Type: Article

References (27)

1. J.-L. Lee, and R.E. Levin Detection of 5 CFU/g of Escherichia coli O157:H7 on lettuce using activated charcoal and real-time PCR without enrichment Food Microbiol. 28 2011 562 567
2. I.G. Wilson Inhibition and facilitation of nucleic acid amplification Appl. Environ. Microbiol. 63 1997 3741 3751 (Pubitemid 27411322)
3. K.L. Johnson, D. Zheng, S. Kaewnum, C.L. Reid, and T. Burr Development of a magnetic capture hybridization real-time PCR assay for detection of tumorigenic agrobacterium vitis in grapevines Phytopathology 103 2013 633 640
4. U. Schlecht, A. Malave, T. Gronewold, M. Tewes, and M. Lohndorf Comparison of antibody and aptamer receptors for the specific detection of thrombin with a nanometer gap-sized impedance biosensor Anal. Chim. Acta 573-574 2006 65 68 (Pubitemid 44066921)
5. S.D. Jayasena Aptamers: an emerging class of molecules that rival antibodies in diagnostics Clin. Chem. 45 1999 1628 1650 (Pubitemid 29419030)
6. T. Mairal, V.C. Ozalp, P. Lozano, M. Sanchez, I. Mir, M. Katakis, and C.K. O'Sullivan Aptamers: molecular tools for analytical applications Anal. Bioanal. Chem. 390 2008 989 1007 (Pubitemid 351207799)
7. V.C. Ozalp, K. Bilecen, M. Kavruk, and H.A. Oktem Antimicrobial aptamers for detection and inhibition of microbial pathogen growth Future Microbiol. 8 2013 1 15
8. A. Pinto, M.C. Bermudo Redondo, V.C. Ozalp, and C.K. O'Sullivan Real-time apta-PCR for 20 000-fold improvement in detection limit Mol. BioSyst. 5 2009 548 553
9. H.J. Lee, B.C. Kim, K.W. Kim, Y.K. Kim, J. Kim, and M.K. Oh A sensitive method to detect Escherichia coli based on immunomagnetic separation and real-time PCR amplification of aptamers Biosens. Bioelectron. 24 2009 3550 3555
10. H. Dwivedi, R.D. Smiley, and L.-A. Jaykus Selection of DNA aptamers for capture and detection of Salmonella typhimurium using a whole-cell SELEX approach in conjunction with cell sorting Appl. Microbiol. Biotechnol. 97 2013 3677 3686
11. N. Duan, S. Wu, C. Zhu, X. Ma, Z. Wang, Y. Yu, and Y. Jiang Dual-color upconversion fluorescence and aptamer-functionalized magnetic nanoparticles-based bioassay for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureus Anal. Chim. Acta 723 2012 1 6
12. P. Delaquis, S. Bach, and L.-D. Dinu Behavior of Escherichia coli O157:H7 in leafy vegetables J. Food Protect. 174 70 2007 1966 1974 (Pubitemid 47237423)
13. K.K. Bradley, J.M. Willams, L.J. Burnsed, M.B. Lytle, M.D. McDermott, R.K. Mody, A. Bhattarai, S. Mallonee, E.W. Piercefield, C.K. McDonald-Hamm, and L.K. Smithee Epidemiology of a large restaurant-associated outbreak of Shiga toxin-producing Escherichia coli O111:NM Epidemiol. Infect. 140 2012 1644 1654
14. M.Y. ArIca, and G. Bayramoǧlu Polyethyleneimine-grafted poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible glucose oxidase immobilization Biochem. Eng. J. 20 2004 73 77 (Pubitemid 38749284)
15. G. Bayramoglu, M. Yilmaz, and M. Yakup Arica, Preparation and characterization of epoxy-functionalized magnetic chitosan beads: laccase immobilized for degradation of reactive dyes Bioprocess Biosyst. Eng. 33 2010 439 448
16. G. Bayramoǧlu, B. AltIntaş, and M.Y. ArIca Reversible immobilization of uricase on conductive polyaniline brushes grafted on polyacrylonitrile film Bioprocess Biosyst. Eng. 34 2011 127 134
17. G. Bayramoglu, and M. Yakup Arica, Poly(methyl methacrylate-glycidiyl methacrylate) film with immobilized iminodiacetic acid and Cu(II) ion: for protein adsorption Fibers Polym. 13 2012 1225 1232
18. G. Bayramoglu, and M. Yakup Arica P(HPMA/EGDMA) beads grafted with fibrous chains by SI-ATRP method: agmatine functionalized affinity beads for selective separation of serum albumin Bioprocess Biosyst. Eng. 2013 1 11 10.1007/s00449-013-0987-2
19. M. Yakup Arica, H. Soydogan, and G. Bayramoglu Reversible immobilization of Candida rugosa lipase on fibrous polymer grafted and sulfonated p(HEMA/EGDMA) beads Bioprocess Biosyst. Eng. 33 2010 227 236
20. H.A. Oktem, G. Bayramoglu, V.C. Ozalp, and M.Y. Arica Single-step purification of recombinant Thermus aquaticus DNA polymerase using DNA-aptamer immobilized novel affinity magnetic beads Biotechnol. Prog. 23 2007 146 154 (Pubitemid 46293498)
21. A.W. Paton, and J.C. Paton Detection and characterization of shiga toxigenic Escherichia coli by using multiplex PCR assays forstx 1, stx 2, eaeA, enterohemorrhagic E. coli hlyA, rfb O111, and rfb O157 J. Clin. Microbiol. 36 1998 598 602 (Pubitemid 28060342)
22. J. Kim, Studies on PCR-based rapid detection systems for Salmonella spp. in: B.S.M. Mahmoud (Ed.), Salmonella - a dangerous foodborne pathogen, Intech, 2012.
23. R. Joshi, H. Janagama, H.P. Dwivedi, T. Kumar, L.A. Jaykus, J. Schefers, and S. Sreevatsan Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovars Mol. Cell. Probes 23 2009 20 28
24. W.H. Wu, M. Li, Y. Wang, H.X. Ouyang, L. Wang, C.X. Li, Y.C. Cao, Q.H. Meng, and J.X. Lu Aptasensors for rapid detection of Escherichia coli O157:H7 and Salmonella typhimurium Nanoscale Res. Lett. 7 2012 658
25. V.C. Ozalp, G. Bayramoglu, M.Y. Arica, and H.A. Oktem Design of a core-shell type immunomagnetic separation system and multiplex PCR for rapid detection of pathogens from food samples Appl. Microbiol. Biotechnol. 97 2013 9541 9551
26. S. Patil, A. Sandberg, E. Hecker, W. Self, and S. Seal Protein adsorption and cellular uptake of cerium oxide nanoparticles as a function of zeta potential Biomaterials 28 2009 4600 4607 (Pubitemid 47259179)
27. Xiao X.,Yang X.,Liu T.,Chen Z.,Chen L.,Deng L., Preparing a highly specific inert immunomolecular-magnetic beads for rapid detection and separation of S. aureus and G Streptococcus, Appl. Microbiol. Biotechnol., 2007, 75, 1209.