Single-step purification of recombinant Thermus aquaticus DNA polymerase using DNA-aptamer immobilized novel affinity magnetic beads

Biotechnology Progress

Volumn 23, Issue 1, 2007, Pages 146-154

  Oktem, Huseyin Avni ^a   Bayramoglu, Gulay ^b   Ozalp, V Cengiz ^a   Arica, M Yakup ^b  

^a MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

^b KIRIKKALE UNIVERSITY   (Turkey)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; ENZYME IMMOBILIZATION; GENETIC ENGINEERING; MAGNETIC FIELD EFFECTS; PARAMETER ESTIMATION; PURIFICATION; SURFACE REACTIONS;

DNA APTAMER; MAGNETIC BEADS; POLYMERASE; THERMUS AQUATICUS DNA POLYMERASE (TAQ-POLYMERASE);

ENZYMES;

APTAMER; DNA DIRECTED DNA POLYMERASE; IMMOBILIZED ENZYME; RECOMBINANT PROTEIN;

CHEMISTRY; CONFERENCE PAPER; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; IMMUNOMAGNETIC SEPARATION; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PHYSIOLOGY; THERMUS;

APTAMERS, NUCLEOTIDE; DNA-DIRECTED DNA POLYMERASE; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; IMMUNOMAGNETIC SEPARATION; RECOMBINANT PROTEINS; THERMUS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; THERMUS AQUATICUS;

EID: 33847098767     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp0602505     Document Type: Conference Paper

References (43)

1. Mosing, R. K.; Mendonsa, S. D.; Bowser, M. T. Capillary electrophoresis-SELEX selection of aptamers with affinity for HIV-1 reverse transcriptase. Anal. Chem. 2005, 77, 6107-6112.
2. Tuerk, C.; Gold, L. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 1990, 249, 505-510.
3. Chaloin, L.; Lehmann, M. J.; Sczakiel, G.; Restle, T. Endogenous expression of a high-affinity pseudoknot RNA aptamer suppresses replication of HIV-1. Nucleic Acids Res. 2002, 30, 4001-4008.
4. Yamamoto-Fujita, R.; Kumar, P. K. R. Aptamer-derived nucleic acid oligos: Applications to develop nucleic acid chips to analyze proteins and small ligands. Anal. Chem. 2005, 77, 5440-5466.
5. Murphy, M. B.; Fuller, S. T.; Richardson, P. M.; Doyle, S. A. An improved method for the in vitro evolution of aptamers and applications in protein detection and purification. Nucleic Acids Res. 2003, 31, e110.
6. Berezovski, M.; Nutiu, R.; Li, Y.; Krylov, S. N. Affinity analysis of a protein-aptamer complex using nonequilibrium capillary electrophoresis of equilibrium mixtures. Anal. Chem. 2003, 75, 1382-1386.
7. Plummer, K. A.; Carothers, J. M.; Yoshimura, M.; Szostak, J. W.; Verdine, G. L. In vitro selection of RNA aptamers against a composite small molecule-protein surface. Nucleic Acids Res. 2005, 33, 5602-5610.
8. Romig, T. S.; Bell, C.; Drolet, D. W. Aptamer affinity chromatography: combinatorial chemistry applied to protein purification. J. Chromatogr. B 1999, 731, 275-284.
9. Ditsch, A.; Yin, J.; Laibinis, P. E.; Wang, D. I. C.; Hatton, T. A. Ion-exchange purification of proteins using magnetic nanoclusters. Biotechnol. Prog. 2006, 22, 1153-1162.
10. Benes, J.; Horak, D.; Svec, F. Methacrylate-based chromatographic media. J. Sep. Sci. 2005, 28, 1855-1875.
11. Sun, L.; Dai, J.; Baker, G. L.; Bruening, M. L. High-capacity, protein-binding membranes based on polymer brushes grown in porous substrates. Chem. Mater. 2006; 18, 4033-4039.
12. Zhang, Z.; Zhang, L.; Chen, L.; Chen, L.; Wan, Q.-H. Synthesis of novel porous magnetic silica microspheres as adsorbents for isolation of genomic DNA. Biotechnol. Prog. 2006, 22, 514-518.
13. Arica, M. Y.; Yilmaz, M.; Yalcin, E., Bayramoglu, G. Affinity membrane chromatography: Relationship of dye-ligand type to surface polarity and their effect on lysozyme separation and purification. J. Chromatogr. B 2004, 805, 315-323.
14. Tsai, S.-Y.; Lin, S.-C.; Suen, S.-Y.; Hsu, W.-H. Effect of number of poly(His) tags on the adsorption of engineered proteins on immobilized metal affinity chromatography adsorbents. Process Biochem. 2006, 41, 2058-2067.
15. Bayramoglu, G.; Arica, M. Y. Surface energy components of a dye-ligand immobilized pHEMA membranes: Effects of their molecular attracting forces for non-covalent interactions with IgG and HSA in aqueous media. Int. J. Biol. Macromol. 2005, 37, 249-256.
16. Tsang, S. C.; Yu, C. H.; Gao, X.; Tam, K. Silica-encapsulated nanomagnetic particle as a new recoverable biocatalyst carrier. J. Phys. Chem. B 2006, 110, 16914-16922.
17. Liu, Y.; Tang, X.; Liu, F.; Li, K. Selection of ligands for affinity chromatography using quartz crystal biosensor. Anal. Chem. 2005, 77, 4248-4256.
18. Mendonsa, S. D.; Bowser, M. T. In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Anal. Chem. 2004, 76, 5387-5392.
19. Amaral, I. P. G.; Carneiro-da-Cunha, M.-G.; Carvalho, Jr., L. B.; Bezerra, R. S.; Fish trypsin immobilized on ferromagnetic Dacron. Process Biochem. 2006, 41, 1213-1216.
20. Huang, S.-H.; Liao, M.-H.; Chen, D.-H. Direct binding and characterization of lipase onto magnetic nanoparticles. Biotechnol. Prog. 2003, 19, 1095-1100.
21. Khng, H. P.; Cunliffe, D.; Davies, S.; Turner, N. A.; Vulfson, E. N. The synthesis of sub-micron magnetic particles and their use for preparative purification of proteins. Biotechnol. Bioeng. 1998, 60, 419-424.
22. Lee, I. S.; Lee, N.; Park, J.; Kim, B. H.; Yi, Y.-W.; Kim, T.; Kim, T. K.; Lee, I. H.; Paik, S. R.; Hyeon, T. Ni/NiO Core/shell nanoparticles for selective binding and magnetic separation of histidine-tagged proteins. J. Am. Chem. Soc. 2006, 128, 10658-10659.
23. Arica, M. Y.; Yavuz, H.; Patir, S.; Denizli, A. Immobilization of glucoamylase onto spacer-arm attached magnetic poly(methylmethacrylate) microspheres: Characterization and application to a continuous flow reactor. J. Mol. Catal. B 2000, 11, 127-138.
24. Yang, C.; Liu, H.; Guan, Y.; Xing, J.; Liu, J.; Shan, G. Preparation of magnetic poly(methylmethacrylate-divinylbenzene-glycidylmethacrylate) microspheres by spraying suspension polymerization and their use for protein adsorption. J. Magn. Magn. Mater. 2005, 293, 187-192.
25. Chang, C.-S.; Suen, S.-Y. Modification of porous alumina membranes with n-alkanoic acids and their application in protein adsorption, J. Membr. Sci. 2006, 275, 70-81.
26. Pluthero, F. G. Rapid purification of high-activity Taq DNA polymerase. Nucleic Acids Res. 1993, 21, 4850-4857.
27. Bayramoglu, G.; Kaya, B.; Arica, M. Y. Immobilization of Candida rugosa lipase onto spacer-arm attached poly(GMA-HEMA-EGDMA) microspheres. Food Chem. 2005, 92, 261-268.
28. Arica, M. Y.; Bayramoglu, G. Polyethyleneimine-grafted poly-(hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible glucose oxidase immobilization. Biochem. Eng. J. 2004, 20, 73-77.
29. Arica, M. Y.; Bayramoglu, G.; Bicak. N. Characterisation of tyrosinase immobilised onto spacer-arm attached glycidyl methacrylate-based reactive microbeads. Process Biochem. 2004, 39, 2007-2017.
30. Finotelli, P. V.; Morales, M. A.; Rocha-Leao, M. H.; Baggio-Saitovitch, E. M. Rossi, A. M. Magnetic studies of iron(III) nanoparticles in alginate polymer for drug delivery applications. Mater. Sci. Eng. C 2004, 24, 625-629.
31. 4/carbon using hydrothermal reaction. J. Magn. Magn. Mater. 2006, 302, 397-4004.
32. 2 superconductors, Supercond. Sci. Technol. 2002, 15 1479-1485.
33. Bid, S.; Banerjee, A.; Kumar, S.; Pradhan, S. K.; De, U., Benerjee, D. Nanophase iron oxides by ball-mill grinding and their Mössbauer characterization J. Alloys Compd. 2001, 326, 292-297.
34. Kamali-M, S.; Ericsson, T.; Wappling, R. Characterization of iron oxide nanoparticles by Mössbauer spectroscopy. Thin Solid Films 2006, 515 721-723.
35. 3 particles determined with the Mössbauer effect Phys. Rev. B 1966, 142, 327-333.
36. Selvam, P.; Dapurkar, S. E.; Badamali, S. K.; Murugasan, M.; Kuwano, H. Coexistence of paramagnetic and superparamagnetic Fe(III) in mesoporous MCM-41 silicates. Catal. Today 2001, 68, 69-74.
37. Bayramoglu, G.; Arica, M. Y. Procion Green H-4G immobilized on a new IPN hydrogel membrane composed of poly(2-hydroxyethylmethacrylate)/chitosan: Preparation and its application to the adsorption of lysozyme. Colloids Surf., A 2002, 202, 41-52.
38. Bayramoglu, G.; Yilmaz, M.; Arica, M. Y. Affinity dye-ligand poly(hydroxyethyl methacrylate)/chitosan composite membrane for adsorption lysozyme and kinetic properties. Biochem. Eng. J. 2003, 13, 35-42.
39. Burrows, J. A.; Goward, C. R. Preparation of DNA polymerase from Bacillus caldotenax. J. Chromatogr. 1993, 639, 75-80.
40. Formaneck, M. S.; Ma, L.; Cui, Q. Effects of temperature and salt concentration on the structural stability of human lymphotactin: Insights from molecular simulations. J. Am. Chem. Soc.2006; 128, 9506-9517.
41. Rustemeier, O.; Killmann, E. Electrostatic interactions and stability of poly-L-lysine covered polystyrene latex particles investigated by dynamic light scattering. J. Colloid Interface Sci. 1997, 290, 360-370.
42. Lin, F.-Y.; Chen, W.-Y.; Hearn, M. T. W. Thermodynamic analysis of the interaction between proteins and solid surfaces: application to liquid chromatography. J. Mol. Recognit. 2002, 15, 55-93.
43. Chang, Y.-K.; Huang, R.-Z.; Lin, S.-Y.; Chiu, S.-J.; Tsai, J.-C. Equilibrium study of immobilized lysozyme on the extrudate-shaped NaY zeolite. Biochem. Eng. J. 2006, 28, 1-9.