A sensitive method to detect Escherichia coli based on immunomagnetic separation and real-time PCR amplification of aptamers

Biosensors and Bioelectronics

Volumn 24, Issue 12, 2009, Pages 3550-3555

  Lee, Hye Jin ^a   Kim, Byoung Chan ^b   Kim, Kyung Woo ^a   Kim, Young Keun ^a   Kim, Jungbae ^a   Oh, Min Kyu ^a  

^a Korea University   (South Korea)

^b Institute Pasteur Korea   (South Korea)

Author keywords

Aptamers; Immunomagnetic separation; Microorganism detection; Real time RT PCR

Indexed keywords

APTAMER; APTAMERS; CELL LYSIS; E. COLI; HIGHLY SENSITIVE; IMMUNOMAGNETIC SEPARATION; MAGNETIC BEADS; MICROORGANISM DETECTION; POLYMERASE CHAIN REACTION; REAL-TIME PCR; REAL-TIME RT-PCR; REVERSE-TRANSCRIPTASE; SAMPLE PREPARATION; SENSITIVE DETECTION; SENSITIVE METHOD; SIGNAL AMPLIFICATIONS; SPECIFIC BINDING; WIDE DYNAMIC RANGE;

ANTIBODIES; BIOMOLECULES; ESCHERICHIA COLI; MAGNETIC SEPARATORS; MICROORGANISMS; NUCLEIC ACIDS; RNA; SEPARATION; SIGNAL PROCESSING; TARGETS;

AMPLIFICATION;

APTAMER; BACTERIAL RNA;

ARTICLE; BACTERIUM DETECTION; CONTROLLED STUDY; CYTOLYSIS; ESCHERICHIA COLI; IMMUNOMAGNETIC SEPARATION; NONHUMAN; NUCLEIC ACID AMPLIFICATION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

APTAMERS, NUCLEOTIDE; BIOSENSING TECHNIQUES; CELL SEPARATION; COLONY COUNT, MICROBIAL; DNA, BACTERIAL; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; ESCHERICHIA COLI; IMMUNOMAGNETIC SEPARATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI;

EID: 67650021765     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bios.2009.05.010     Document Type: Article

References (34)

1. Abeliovich H. Biophys. J. 89 (2005) 76-79
2. Acheson D.W.K., Lincicome L.L., DeBreucker S., and Keusch G.T. J. Food Protect. 59 (1996) 344-349
3. Bang G.S., Cho S., and Kim B.G. Biosens. Bioelectron. 21 (2005) 863-870
4. Barlen B., Mazumdar S.D., Lezrich O., Kampfer P., and Keusgen M. Sensors 7 (2007) 1427-1446
5. Bruno J.G., and Kiel J.L. Biosens. Bioelectron. 14 (1999) 457-464
6. Chen F., Zhou J., Luo F.L., Mohammed A.B., and Zhang X.L. Biochem. Biophys. Res. Commun. 357 (2007) 743-748
7. Daniels D.A., Chen H., Hicke B.J., Swiderek K.M., and Gold L. Proc. Natl. Acad. Sci. U.S.A. 100 (2003) 15416-15421
8. Ellington A.D., and Szostak J.W. Nature 346 (1990) 818-822
9. Fischer N.O., Tarasow T.M., and Tok J.B.H. Anal. Biochem. 373 (2008) 121-128
10. Fu Z., Rogelj S., and Kieft T.L. Int. J. Food Microbiol. 99 (2005) 47-57
11. He P.L., Shen L., Cao Y.H., and Lia D.F. Anal. Chem. 79 (2007) 8024-8029
12. Hermann T., and Patel D.J. Science 287 (2000) 820-825
13. Ho J.A.A., and Hsu H.W. Anal. Chem. 75 (2003) 4330-4334
14. Huang Y.F., Chang H.T., and Tan W.H. Anal. Chem. 80 (2008) 567-572
15. Ikanovic M., Rudzinski W.E., Bruno J.G., Allman A., Carrillo M.P., Dwarakanath S., Bhahdigadi S., Rao P., Kiel J.L., and Andrews C.J. J. Fluoresc. 17 (2007) 193-199
16. Kalele S.A., Kundu A.A., Gosavi S.W., Deobagkar D.N., Deobagkar D.D., and Kulkarni S.K. Small 2 (2006) 335-338
17. Kilic A., Ertas H.B., Muz A., Ozbey G., and Kalender H. Turk. J. Vet. Anim. Sci. 31 (2007) 215-218
18. Lato S.M., Boles A.R., and Ellington A.D. Chem. Biol. 2 (1995) 291-303
19. Liu J.W., and Lu Y. Adv. Mater. 18 (2006) 1667-1671
20. Macaya R.F., Schultze P., Smith F.W., Roe J.A., and Feigon J. Proc. Natl. Acad. Sci. U.S.A. 90 (1993) 3745-3749
21. Mahmoud K.A., Long Y.T., Schatte G., and Kraatz H.B. Eur. J. Inorg. Chem. 1 (2005) 173-180
22. Pan Q., Zhang X.L., Wu H.Y., He P.W., Wang F.B., Zhang M.S., Hu J.M., Xia B., and Wu J.G. Antimicrob. Agents Chemother. 49 (2005) 4052-4060
23. Pan W.H., Craven R.C., Qiu Q., Wilson C.B., Wills J.W., Golovine S., and Wang J.F. Proc. Natl. Acad. Sci. U.S.A. 92 (1995) 11509-11513
24. So H.M., Park D.W., Jeon E.K., Kim Y.H., Kim B.S., Lee C.K., Choi S.Y., Kim S.C., Chang H., and Lee J.O. Small 4 (2008) 197-201
25. Stojanovic M.N., de Prada P., and Landry D.W. J. Am. Chem. Soc. 123 (2001) 4928-4931
26. Stoltenburg R., Reinemann C., and Strehlitz B. Biomol. Eng. 24 (2007) 381-403
27. Tuerk C., and Gold L. Science 249 (1990) 505-510
28. Walker, J.M., 1994. The Protein Protocols Handbook, 2nd ed. Humana Press, Totowa, NJ.
29. Wang C., and Irudayaraj J. Small 4 (2008) 2204-2208
30. Wang L.J., Wu C.S., Hu Z.Y., Zhang Y.F., Li R., and Wang P. J. Zhejiang Univ. Sci. B 9 (2008) 121-131
31. Yang L.T., Fung C.W., Cho E.J., and Ellington A.D. Anal. Chem. 79 (2007) 3320-3329
32. Yifrach O. Biophys. J. 87 (2004) 822-830
33. Zhang H.Q., Wang Z.W., Li X.F., and Le X.C. Angew. Chem. Int. Ed. 45 (2006) 1576-1580
34. Zheng J., Feng W., Lin L., Zhang F., Cheng G., He P., and Fang Y. Biosens. Bioelectron. 23 (2007) 341-347