Development of a highly sensitive, high-throughput assay for glycosyltransferases using enzyme-coupled fluorescence detection

Analytical Biochemistry

Volumn 447, Issue 1, 2014, Pages 146-155

  Kumagai, Kazuo ^a,b   Kojima, Hirotatsu ^a   Okabe, Takayoshi ^a   Nagano, Tetsuo ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b DAINIPPON SUMITOMO PHARMA CO LTD   (Japan)

Author keywords

Enzyme coupled assay; Fluorescence assay; Glycosyltransferase; High throughput screening; Nucleotide determination

Indexed keywords

COSTS; DRUG PRODUCTS; FLUORESCENCE; MOLECULES; NUCLEOTIDES;

FLUORESCENCE ASSAY; FLUORESCENCE DETECTION; FLUORESCENCE MEASUREMENTS; GLUCOSE-6-PHOSPHATE DEHYDROGENASE; GLYCOSYL TRANSFERASE; GLYCOSYLATION REACTIONS; HIGH THROUGHPUT SCREENING; HIGH-THROUGHPUT ASSAYS;

ENZYMES;

3 (3,4 DIHYDROXYPHENYL) 2 METHYL ALANINE; 3,4 DIHYDRO PHENYLALANINE; 6 HYDROXYDOPA; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; AURINTRICARBOXYLIC ACID; BETA 1,4 GALACTOSYLTRANSFERASE; CEFAPIRIN; CYTIDINE PHOSPHATE; DIHYDROLIPOAMIDE DEHYDROGENASE; GALACTOSYLTRANSFERASE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GLYCOSYLTRANSFERASE; GLYCOSYLTRANSFERASE INHIBITOR; GUANOSINE DIPHOSPHATE; HEXOKINASE; HISPIDIN; ME 3,4 DEPHOSTATIN; NUCLEOTIDE; QUERCETIN; REAGENT; RESAZURIN; RESORUFIN; TAXIFOLIN; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE;

ARTICLE; CHEMICAL REACTION; COST; ENZYME ASSAY; ENZYME COUPLED FLUORESCENCE ASSAY; ENZYME MECHANISM; ENZYME PHOSPHORYLATION; FALSE POSITIVE RESULT; FLUORESCENCE ANALYSIS; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; HUMAN; INTERMETHOD COMPARISON; PRIORITY JOURNAL;

ENZYME-COUPLED ASSAY; FLUORESCENCE ASSAY; GLYCOSYLTRANSFERASE; HIGH-THROUGHPUT SCREENING; NUCLEOTIDE DETERMINATION;

COST-BENEFIT ANALYSIS; DRUG EVALUATION, PRECLINICAL; ENZYME ASSAYS; ENZYME INHIBITORS; FLUORESCENCE; GLYCOSYLTRANSFERASES; HUMANS; LUMINESCENT MEASUREMENTS; NUCLEOTIDES; REPRODUCIBILITY OF RESULTS; SMALL MOLECULE LIBRARIES;

EID: 84891524320     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.11.025     Document Type: Article

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