Testing the limits of 454 pyrotag sequencing: Reproducibility, quantitative assessment and comparison to T-RFLP fingerprinting of aquifer microbes

PLoS ONE

Volumn 7, Issue 7, 2012, Pages

  Pilloni, Giovanni ^a   Granitsiotis, Michael S ^a   Engel, Marion ^a   Lueders, Tillmann ^a  

^a INSTITUTE OF EPIDEMIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CONTIG; DNA; RNA 16S; TAR;

ALIIVIBRIO FISCHERI; AMPLICON; AQUIFER; ARTICLE; BACTERIUM CULTURE; COMMUNITY STRUCTURE; DNA FINGERPRINTING; GENETIC VARIABILITY; GERMANY; INTERMETHOD COMPARISON; MICROBIAL COMMUNITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PYROSEQUENCING; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SEDIMENT; WATER CONTAMINATION;

BACTERIA; DNA FINGERPRINTING; DNA, BACTERIAL; GENE LIBRARY; GENES, BACTERIAL; GENETIC VARIATION; GROUNDWATER; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; REPRODUCIBILITY OF RESULTS; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

BACTERIA (MICROORGANISMS);

EID: 84863739532     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0040467     Document Type: Article

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