A rapid MALDI-TOF mass spectrometry workflow for Drosophila melanogaster differential neuropeptidomics

Molecular Brain

Volumn 6, Issue 1, 2013, Pages

  Salisbury, Joseph P ^a,b   Boggio, Kristin J ^a,e   Hsu, Yun Wei A ^a,f   Quijada, Jeniffer ^b   Sivachenko, Anna ^a   Gloeckner, Gabriele ^c   Kowalski, Paul J ^d   Easterling, Michael L ^d   Rosbash, Michael ^a   Agar, Jeffrey N ^b  

^a BRANDEIS UNIVERSITY   (United States)

^b NORTHEASTERN UNIVERSITY   (United States)

^c UNIVERSITY OF REGENSBURG   (Germany)

^d BRUKER DALTONIK GMBH   (Germany)

^e UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^f SEATTLE CHILDREN S RESEARCH INSTITUTE   (United States)

Author keywords

Drosophila melanogaster; MALDI TOF; Neuropeptide like precursor 1; Neuropeptides; Neuropeptidomics; NPLP1; Pigment dispersing factor; Tandem mass spectrometry

Indexed keywords

NEUROPEPTIDE; PIGMENT DISPERSING FACTOR; UNCLASSIFIED DRUG; DROSOPHILA PROTEIN; ION;

ADULT; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DROSOPHILA MELANOGASTER; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PEPTIDOMICS; PRIORITY JOURNAL; REPRODUCIBILITY; SIGNAL TRANSDUCTION; WORKFLOW; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; CONFIDENCE INTERVAL; ISOLATION AND PURIFICATION; ISOTOPE LABELING; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; PROCEDURES; PROTEOMICS; SIGNAL PROCESSING;

DROSOPHILA MELANOGASTER;

AMINO ACID SEQUENCE; ANIMALS; CONFIDENCE INTERVALS; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; IONS; ISOTOPE LABELING; MOLECULAR SEQUENCE DATA; NEUROPEPTIDES; PROTEOMICS; SIGNAL PROCESSING, COMPUTER-ASSISTED; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84890983180     PISSN: None     EISSN: 17566606     Source Type: Journal    
DOI: 10.1186/1756-6606-6-60     Document Type: Article

References (40)

1. Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two-dimensional capillary liquid chromatography quadrupole time-of-flight mass spectrometry. Baggerman G, Boonen K, Verleyen P, De Loof A, Schoofs L, J Mass Spectrom 2005 40 2 250 260 10.1002/jms.744 15706625
2. Neuropeptide signaling in insects. Altstein M, Nassel DR, Adv Exp Med Biol 2010 692 155 165 10.1007/978-1-4419-6902-6-8 21189678
3. Neuropeptide biology in Drosophila. Clynen E, Reumer A, Baggerman G, Mertens I, Schoofs L, Adv Exp Med Biol 2010 692 192 210 10.1007/978-1-4419-6902- 6-10 21189680
4. New vistas for melanocortins. Finally, an explanation for their pleiotropic functions. Strand FL, Ann N Y Acad Sci 1999 897 1 16 10.1111/j.1749-6632.1999.tb07874.x 10676431
5. Neuromodulatory function of neuropeptides in the normal CNS. Merighi A, Salio C, Ferrini F, Lossi L, J Chem Neuroanat 2011 42 4 276 287 10.1016/j.jchemneu.2011.02.001 21385606
6. Single-cell peptidomics of drosophila melanogaster neurons identified by Gal4-driven fluorescence. Neupert S, Johard HA, Nassel DR, Predel R, Anal Chem 2007 79 10 3690 3694 10.1021/ac062411p 17439240
7. Peptidomics of the larval Drosophila melanogaster central nervous system. Baggerman G, Cerstiaens A, De Loof A, Schoofs L, The Journal of biological chemistry 2002 277 43 40368 40374 10.1074/jbc.M206257200 12171930
8. Peptidomics in Drosophila melanogaster. Schoofs L, Baggerman G, Brief Funct Genomic Proteomic 2003 2 2 114 120 10.1093/bfgp/2.2.114 15239932
9. Peptidomics of CNS-associated neurohemal systems of adult Drosophila melanogaster: a mass spectrometric survey of peptides from individual flies. Predel R, Wegener C, Russell WK, Tichy SE, Russell DH, Nachman RJ, J Comp Neurol 2004 474 3 379 392 10.1002/cne.20145 15174081
10. Neuropeptides and neuropeptide receptors in the Drosophila melanogaster genome. Hewes RS, Taghert PH, Genome Res 2001 11 6 1126 1142 10.1101/gr.169901 11381038
11. Neuropeptides and their precursors in the fruitfly, Drosophila melanogaster. Vanden Broeck J, Peptides 2001 22 2 241 254 10.1016/S0196-9781(00) 00376-4 11179818
12. The regulation of circadian rhythms in the fly's visual system: involvement of FMRFamide-like neuropeptides and their relationship to pigment dispersing factor in Musca domestica and Drosophila melanogaster. Pyza E, Meinertzhagen IA, Neuropeptides 2003 37 5 277 289 10.1016/j.npep.2003.06.001 14607105
13. Defining the contribution of select neuropeptides and their receptors in regulating sesquiterpenoid biosynthesis by Drosophila melanogaster ring gland/corpus allatum through RNAi analysis. Wang C, Zhang J, Tobe SS, Bendena WG, Gen Comp Endocrinol 2012 176 3 347 353 10.1016/j.ygcen.2011.12.039 22245290
14. A pdf neuropeptide gene mutation and ablation of PDF neurons each cause severe abnormalities of behavioral circadian rhythms in Drosophila. Renn SC, Park JH, Rosbash M, Hall JC, Taghert PH, Cell 1999 99 7 791 802 10.1016/S0092-8674(00)81676-1 10619432
15. From the genome to the proteome: uncovering peptides in the Apis brain. Hummon AB, Richmond TA, Verleyen P, Baggerman G, Huybrechts J, Ewing MA, Vierstraete E, Rodriguez-Zas SL, Schoofs L, Robinson GE, et al. Science 2006 314 5799 647 649 10.1126/science.1124128 17068263
16. Genomics, transcriptomics, and peptidomics of neuropeptides and protein hormones in the red flour beetle Tribolium castaneum. Li B, Predel R, Neupert S, Hauser F, Tanaka Y, Cazzamali G, Williamson M, Arakane Y, Verleyen P, Schoofs L, et al. Genome Res 2008 18 1 113 122 18025266
17. Ecdysteroids, juvenile hormone and insect neuropeptides: Recent successes and remaining major challenges. De Loof A, Gen Comp Endocrinol 2008 155 1 3 13 10.1016/j.ygcen.2007.07.001 17716674
18. Multiple neuropeptides in the Drosophila antennal lobe suggest complex modulatory circuits. Carlsson MA, Diesner M, Schachtner J, Nassel DR, J Comp Neurol 2010 518 16 3359 3380 10.1002/cne.22405 20575072
19. Analysis of Neuropeptide expression and localization in adult drosophila melanogaster central nervous system by affinity cell-capture mass spectrometry. Yew JY, Wang Y, Barteneva N, Dikler S, Kutz-Naber KK, Li L, Kravitz EA, J Proteome Res 2009 8 3 1271 1284 10.1021/pr800601x 19199706
20. The receptor guanylate cyclase Gyc76C and a peptide ligand, NPLP1-VQQ, modulate the innate immune IMD pathway in response to salt stress. Overend G, Cabrero P, Guo AX, Sebastian S, Cundall M, Armstrong H, Mertens I, Schoofs L, Dow JA, Davies SA, Peptides 2012 34 1 209 218 10.1016/j.peptides.2011.08.019 21893139
21. Quantitative peptidomics reveal brain peptide signatures of behavior. Brockmann A, Annangudi SP, Richmond TA, Ament SA, Xie F, Southey BR, Rodriguez-Zas SR, Robinson GE, Sweedler JV, Proc Natl Acad Sci USA 2009 106 7 2383 2388 10.1073/pnas.0813021106 19179284
22. Comparative neuropeptidomic analysis of food intake via a multi-faceted mass spectrometric approach. Chen R, Hui L, Cape SS, Wang J, Li L, ACS Chem Neurosci 2010 1 3 204 214 10.1021/cn900028s 20368756
23. Profiling of diet-induced Neuropeptide changes in Rat brain by quantitative mass spectrometry. Frese CK, Boender AJ, Mohammed S, Heck AJR, Adan RAH, Altelaar AFM, Anal Chem 2013 85 9 4594 4604 10.1021/ac400232y 23581470
24. Identification, physiological actions, and distribution of VYRKPPFNGSIFamide (Val1)-SIFamide) in the stomatogastric nervous system of the American lobster Homarus americanus. Christie AE, Stemmler EA, Peguero B, Messinger DI, Provencher HL, Scheerlinck P, Hsu YW, Guiney ME, de la Iglesia HO, Dickinson PS, J Comp Neurol 2006 496 3 406 421 10.1002/cne.20932 16566002
25. Heterogeneity within MALDI samples as revealed by mass spectrometric imaging. Garden RW, Sweedler JV, Anal Chem 2000 72 1 30 36 10.1021/ac9908997 10655631
26. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Brand AH, Perrimon N, Development 1993 118 2 401 415 8223268
27. Relative quantitation of neuropeptides over a thousand-fold concentration range. Hou X, Xie F, Sweedler JV, J Am Soc Mass Spectrom 2012 23 12 2083 2093 10.1007/s13361-012-0481-0 22993045
28. Quantitative measurements of cell-cell signaling peptides with single-cell MALDI MS. Rubakhin SS, Sweedler JV, Anal Chem 2008 80 18 7128 7136 10.1021/ac8010389 18707135
29. Feature extraction and quantification for mass spectrometry in biomedical applications using the mean spectrum. Morris JS, Coombes KR, Koomen J, Baggerly KA, Kobayashi R, Bioinformatics 2005 21 9 1764 1775 10.1093/bioinformatics/ bti254 15673564
30. Direct mass spectrometric peptide profiling and fragmentation of larval peptide hormone release sites in Drosophila melanogaster reveals tagma-specific peptide expression and differential processing. Wegener C, Reinl T, Jansch L, Predel R, J Neurochem 2006 96 5 1362 1374 10.1111/j.1471-4159.2005.03634.x 16441518
31. Deletion of peptide amidation enzymatic activity leads to edema and embryonic lethality in the mouse. Czyzyk TA, Ning Y, Hsu MS, Peng B, Mains RE, Eipper BA, Pintar JE, Dev Biol 2005 287 2 301 313 10.1016/j.ydbio.2005.09.001 16225857
32. Neuropeptide amidation in Drosophila: separate genes encode the two enzymes catalyzing amidation. Kolhekar AS, Roberts MS, Jiang N, Johnson RC, Mains RE, Eipper BA, Taghert PH, J Neurosci 1997 17 4 1363 1376 9006979
33. Expression of a novel neuropeptide, NVGTLARDFQLPIPNamide, in the larval and adult brain of Drosophila melanogaster. Verleyen P, Baggerman G, Wiehart U, Schoeters E, Van Lommel A, De Loof A, Schoofs L, J Neurochem 2004 88 2 311 319 14690519
34. Comparative peptidomics analysis of neural adaptations in rats repeatedly exposed to amphetamine. Romanova EV, Lee JE, Kelleher NL, Sweedler JV, Gulley JM, J Neurochem 2012 123 2 276 287 10.1111/j.1471-4159.2012.07912.x 22860605
35. MALDI mass spectrometry based molecular phenotyping of CNS glial cells for prediction in mammalian brain tissue. Hanrieder J, Wicher G, Bergquist J, Andersson M, Fex-Svenningsen A, Anal Bioanal Chem 2011 401 1 135 147 10.1007/s00216-011-5043-y 21553124
36. Proteomic profiling of intact mycobacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Hettick JM, Kashon ML, Simpson JP, Siegel PD, Mazurek GH, Weissman DN, Anal Chem 2004 76 19 5769 5776 10.1021/ac049410m 15456297
37. Differential regulation of circadian pacemaker output by separate clock genes in Drosophila. Park JH, Helfrich-Forster C, Lee G, Liu L, Rosbash M, Hall JC, Proc Natl Acad Sci U S A 2000 97 7 3608 3613 10.1073/pnas.97.7.3608 10725392
38. Isolation and chronobiological analysis of a neuropeptide pigment-dispersing factor gene in Drosophila melanogaster. Park JH, Hall JC, J Biol Rhythms 1998 13 3 219 228 10.1177/074873098129000066 9615286
39. Transformation of cultured Drosophila melanogaster cells with a dominant selectable marker. Rio DC, Rubin GM, Mol Cell Biol 1985 5 8 1833 1838 3018529
40. Improved peak detection and quantification of mass spectrometry data acquired from surface-enhanced laser desorption and ionization by denoising spectra with the undecimated discrete wavelet transform. Coombes KR, Tsavachidis S, Morris JS, Baggerly KA, Hung MC, Kuerer HM, Proteomics 2005 5 16 4107 4117 10.1002/pmic.200401261 16254928