Performance of skin tests with allergens from B. melitensis B115 and rough B. abortus mutants for diagnosing swine brucellosis

Veterinary Microbiology

Volumn 168, Issue 1, 2014, Pages 161-168

  Dieste Perez L ^a   Blasco, J M ^a   De Miguel, M J ^a   Marin C M ^a   Barberan M ^b   Conde Alvarez R ^c   Moriyon I ^c   Munoz P M ^a  

^a Instituto Agroalimentario de Aragón IA2   (Spain)

^b UNIVERSIDAD DE ZARAGOZA   (Spain)

^c UNIVERSITY OF NAVARRA   (Spain)

Author keywords

Brucella suis; False positive serological reactions; Porcine brucellosis; Skin test

Indexed keywords

BACTERIAL ANTIGEN; ROSE BENGAL;

ADULT; ALLERGIC REACTION; ALLERGY TEST; ANIMAL EXPERIMENT; ARTICLE; BACTERIUM MUTANT; BIOTYPE; BRUCELLA ABORTUS; BRUCELLA MELITENSIS; BRUCELLA SUIS; BRUCELLOSIS; COMPLEMENT FIXATION TEST; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FALSE POSITIVE RESULT; FEMALE; NONHUMAN; SENSITIVITY AND SPECIFICITY; SEROLOGY; SKIN TEST; SOW (SWINE);

ANIMALIA; BRUCELLA; BRUCELLA MELITENSIS; BRUCELLA MELITENSIS BIOVAR ABORTUS; BRUCELLA MELITENSIS BIOVAR SUIS; NEGIBACTERIA; SUIDAE; SUS; YERSINIA ENTEROCOLITICA;

BRUCELLA SUIS; FALSE POSITIVE SEROLOGICAL REACTIONS; PORCINE BRUCELLOSIS; SKIN TEST;

ALLERGENS; ANIMALS; BACTERIAL PROTEINS; BRUCELLA; BRUCELLA ABORTUS; BRUCELLOSIS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; MALE; MUTATION; SENSITIVITY AND SPECIFICITY; SEROLOGIC TESTS; SKIN TESTS; SWINE; SWINE DISEASES;

EID: 84890803086     PISSN: 03781135     EISSN: 18732542     Source Type: Journal    
DOI: 10.1016/j.vetmic.2013.10.024     Document Type: Article

References (41)

1. Abdoel T., Dias I.T., Cardoso R., Smits H.L. Simple and rapid field tests for brucellosis in livestock. Vet. Microbiol. 2008, 130:312-319.
2. Adone R., Muscillo M., La Rosa G., Francia M., Tarantino M. Antigenic, immunologic and genetic characterization of rough strains B. abortus RB51, B. melitensis B115 and B. melitensis B18. PLoS ONE 2011, 6.
3. Blasco J.M., Marín C., Jimenez de de Bagüés M.P., Barberán M., Hernandez A., Molina L., Velasco J., Díaz R., Moriyón I. Evaluation of allergic and serological tests for diagnosing Brucella melitensis infection in sheep. JCM 1994, 32:1835-1840.
4. Burnette W.N. Western Blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 1981, 112:195-203.
5. Cloeckaert A., Zygmunt M., Nicolle J.C., Dubray G., Limet J. O-chain expression in the rough Brucella melitensis strain B115: induction of O-polysaccharide-specific monoclonal antibodies and intracellular localization demonstrated by immunoelectron microscopy. J. Gen. Microbiol. 1992, 138:1211-1219.
6. Cloeckaert A., Zygmunt M.S., Dubray G., Limet J.N. Characterization of O-polysaccharide specific monoclonal-antibodies derived from mice infected with the rough Brucella melitensis strain B115. J. Gen. Microbiol. 1993, 139:1551-1556.
7. Cvetnic Z., Spicic S., Toncic J., Majnaric D., Benic M., Albert D., Thiebaud M., Garin-Bastuji B. Brucella suis infection in domestic pigs and wild boar in Croatia. Rev. Sci. Tech. Off. Int. Epizoot. 2009, 28:1057-1067.
8. De Miguel M.J., Marín C.M., Muñoz P.M., Dieste L., Grilló M.J., Blasco J.M. Development of a selective culture medium for primary isolation of the main Brucella species. J. Clin. Microbiol. 2011, 49:1458-1463.
9. Díaz R. Antigenic relations between Yersinia enterocolitica serotype 9 and other Gram-negative bacterial species (Spanish). Microbiol. Española 1974, 27:1-14.
10. EFSA Opinion of the Panel of Animal Health and Welfare (AHAW) on a request from the commission on porcine brucellosis (Brucella suis). The EFSA Journal 2009, 1144:64-112.
11. Ferris R.A., Schoenbaum M.A., Crawford R.P. Comparison of serologic tests and bacteriologic culture for detection of brucellosis in swine from naturally infected herds. JAVMA 1995, 207:1332-1333.
12. Garin Bastuji B., Hars J., Calvez D., Thiebaud M., Artois M. Brucellosis of domestic pigs. Re-emergence of Brucella suis biovar 2 in France. Epidemiol. Sante Anim. 2000, 38:1-5.
13. Godfroid F., Cloeckaert A., Taminiau B., Danese I., Tibor A., de Bolle X., Mertens P., Letesson J.J. Genetic organisation of the lipopolysaccharide O-antigen biosynthesis region of Brucella melitensis 16M (wbk). Res. Microbiol. 2000, 151:655-668.
14. González D., Grilló M.J., de Miguel M.J., Ali T., Arce-Gorvel V., Delrue R.M., Conde-Álvarez R., Muñoz P., López-Goñi I., Iriarte M., Marín C.M., Weintraub A., Widmalm G., Zygmunt M., Letesson J.J., Gorvel J.P., Blasco J.M., Moriyón I. Brucellosis vaccines: assessment of Brucella melitensis lipopolysaccharide rough mutants defective in core and O-polysaccharide synthesis and export. PLoS ONE 2008, 3:e2760.
15. Grabar P., Williams C.A., Courcon J. Methode immuno-electrophoretic danlyse de melanges de substances antigeniques. Biochim. Biophys. Acta 1955, 17:67-74.
16. Jones L.M., Díaz R., Taylor A.G. Characterization of allergens prepared from smooth and rough Strains of Brucella melitensis. Br. J. Exp. Pathol. 1973, 54:492-508.
17. Jungersen G., Sorensen V., Giese S.B., Stack J.A., Riber U. Differentiation between serological responses to Brucella suis and Yersinia enterocolitica serotype O:9 after natural or experimental infection in pigs. Epidemiol. Infect. 2006, 134:347-357.
18. Kubler-Kielb J., Vinogradov E. The study of the core part and non-repeating elements of the O-antigen of Brucella lipopolysaccharide. Carbohydr. Res. 2013, 366:33-37.
19. Markwell M.A.K., Haas S.M., Bieber L.L., Tolber N.E. A modification of the Lowry procedure to simplify protein determination in membrane lipoprotein samples. Anal. Biochem. 1978, 87:206-210.
20. McGiven J.A. New developments in the immunodiagnosis of brucellosis in livestock and wildlife. Rev. Sci. Tech. (Int. Office of Epizootics) 2013, 32:163-176.
21. McGiven J.A., Nicola A., Commander N.J., Duncombe L., Taylor A.V., Villari S., Dainty A., Thirlwall R., Bouzelmat N., Perrett L.L., Brew S.D., Stack J.A. An evaluation of the capability of existing and novel serodiagnostic methods for porcine brucellosis to reduce false positive serological reactions. Vet. Microbiol. 2012, 160:378-386.
22. Mittal K.R., Tizard I.R. Serologic response of pigs to experimental infection with Yersinia enterocolitica serotype 0:9 and Brucella abortus. Am. J. Vet. Res. 1981, 42:443-446.
23. Monreal D., Grilló M.J., González D., Marín C.M., de Miguel M.J., López-Goñi I., Blasco J.M., Cloeckaert A., Moriyón I. Characterization of Brucella abortus O-polysaccharide and core lipopolysaccharide mutants and demonstration that a complete core is required for rough vaccines to be efficient against Brucella abortus and Brucella ovis in the mouse model. Infect. Immun. 2003, 71:3261-3271.
24. Moreno E., Jones L.M., Berman D.T. Immunochemical characterization of rough Brucella lipopolysacharides. Infect. Immun. 1984, 43:779-782.
25. Muñoz P.M., Marín C.M., Monreal D., González D., Garin-Bastuji B., Díaz R., Mainar-Jaime R.C., Moriyón I., Blasco J.M. Efficacy of several serological tests and antigens for diagnosis of bovine brucellosis in the presence of false-positive serological results due to Yersinia enterocolitica O:9. Clin. Diagn. Lab. Immunol. 2005, 12:141-151.
26. Muñoz P.M., Blasco J.M., Engel B., de Miguel M.J., Marín C.M., Dieste L., Mainar-Jaime R.C. Assessment of performance of selected serological tests for diagnosing brucellosis in pigs. Vet. Immunol. Immunopathol. 2012, 146:150-158.
27. Muñoz P.M., Boadella M., Arnal M., de Miguel M.J., Revilla M., Martínez D., Vicente J., Acevedo P., Oleaga A., Ruiz-Fons F., Marín C.M., Prieto J.M., de la Fuente J., Barral M., Barberán M., de Luco D.F., Blasco J.M., Gortazar C. Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates. BMC Infect. Dis. 2010, 10.
28. Nielsen K., Smith P., Yu W., Nicoletti P., Jungersen G., Stack J., Godfroid J. Serological discrimination by indirect enzyme immunoassay between the antibody response to Brucella sp and Yersinia enterocolitica O:9 in cattle and pigs. Vet. Immunol. Immunopathol. 2006, 109:69-78.
29. Nielsen K., Smith P., Yu W.L., Halbert G. Salmonella enterica Serotype urbana interference with brucellosis serology. J. Immunoassay Immunochem. 2007, 28:289-296.
30. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Chapter 2.8.5 2012, Off. Int. Epizoot, Paris.
31. Pouillot R., Garin-Bastuji B., Gerbier G., Coche Y., Cau C., Dufour B., Moutou F. The brucellin skin test as a tool to discriminate false positive serological reactions in bovine brucellosis. Vet. Res. 1997, 28:365-374.
32. Praud A., Gimenez O., Zanella G., Dufour B., Pozzi N., Antras V., Meyer L., Garin-Bastuji B. Estimation of sensitivity and specificity of five serological tests for the diagnosis of porcine brucellosis. Prev. Vet. Med. 2012, 104:94-100.
33. Radunz B.L., Lepper A.W.D. Suppresion of skin reactivity to bovine tuberculine in repeat tests. Aust. Vet. J. 1985, 62:191-194.
34. Riber U., Jungersen G. Cell-mediated immune responses differentiate infections with Brucella suis from Yersinia enterocolitica serotype O:9 in pigs. Vet. Immunol. Immunopathol. 2007, 116:13-25.
35. Rogers R.J., Cook D.R., Ketterer P.J., Baldock F.C., Blackall P.J., Stewart R.W. An evaluation of three serological tests for antibody to Brucella suis in pigs. Aust. Vet. J. 1989, 66:77-80.
36. Rojas N., Freer E., Weintraub A., Ramírez M., Lind S., Moreno E. Immunochemical identification of Brucella abortus lipopolysaccharide epitopes. Clin. Diagn. Lab. Immunol. 1994, 1:206-213.
37. Saegerman C., Vo T.O., De-Waele L., Gilson D., Bastin A., Dubray G., Flanagan P., Limet J.N., Letesson J.J., Godfroid J. Diagnosis of bovine brucellosis by skin test: conditions for the test and evaluation of its performance. Vet. Rec. 1999, 145:214-218.
38. Thibodeau V., Frost E.H., Quessy S. Development of an ELISA procedure to detect swine carriers of pathogenic Yersinia enterocolitica. Vet. Microbiol. 2001, 82:249-259.
39. Tsai C.M., Frasch C.E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 1982, 199.
40. Velasco J., Díaz R., Grilló M.J., Barberán M., Marín C., Blasco J.M., Moriyón I. Antibody and delayed-type hypersensitivity responses to Ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough Brucella spp. Clin. Diagn. Lab. Immunol. 1997, 4:279-284.
41. Olsen S.C., Garin-Bastuji B., Blasco J.M., Nicola A.M., Samartino L. Brucellosis. Diseases of Swine 2012, 697-708. Wiley-Blackwell, USA. 10th ed. J.J. Zimmerman, L.A. Karriker, A. Ramírez, K.J. Schwartz, G.W. Stevenson (Eds.).