Antigenic, immunologic and genetic characterization of rough strains B.abortus RB51, B.melitensis B115 and B.melitensis B18

PLoS ONE

Volumn 6, Issue 10, 2011, Pages

  Adone, Rosanna ^a   Muscillo, Michele ^a   la Rosa, Giuseppina ^a   Francia, Massimiliano ^a   Tarantino, Michela ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; BACTERIAL POLYSACCHARIDE; BACTERIUM ANTIBODY; RIFAMPICIN; LIPOPOLYSACCHARIDE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBIOTIC RESISTANCE; ANTIBODY RESPONSE; ANTIGEN ANTIBODY REACTION; ANTIGENICITY; ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ISOLATE; BACTERIUM ISOLATION; BACTERIUM MUTANT; BRUCELLA ABORTUS; BRUCELLA MELITENSIS; BRUCELLA MELITENSIS B115; BRUCELLA MELITENSIS B18; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOPLASM; GENE SEQUENCE; IMMUNOGENICITY; MUTATIONAL ANALYSIS; NONHUMAN; NONSENSE MUTATION; NUCLEOTIDE SEQUENCE; STRAIN DIFFERENCE; WZM GENE; ANIMAL; BIOSYNTHESIS; CLASSIFICATION; GENETICS; IMMUNOLOGY; MOUSE; MUTATION; RABBIT;

BRUCELLA; MUS; ORYCTOLAGUS CUNICULUS;

ANIMALS; ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; BRUCELLA ABORTUS; BRUCELLA MELITENSIS; GENES, BACTERIAL; LIPOPOLYSACCHARIDES; MICE; MUTATION; RABBITS;

EID: 80055111549     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0024073     Document Type: Article

References (43)

1. Lapaque N, Moriyon I, Moreno E, Gorvel JP, (2005) Brucella lipopolysaccharide acts as a virulence factor. Curr Opin Microbiol 8: 60-66.
2. Moreno E, Moriyon I, (2002) Brucella melitensis: a nasty bug with hidden credentials for virulence. Proc Natl Acad. Sci USA 99: 1-3.
3. Ugalde J, Czibener C, Feldman MF, Ugalde RA, (2000) Identification and characterization of the Brucella abortus phosphoglucomutase gene: role of the lipopolysaccharide in virulence and intracellular multiplication. Infect Immun 68: 5716-5723.
4. Ugalde JE, Comerci DJ, Leguizamon MS, Ugalde RA, (2003) Evaluation of Brucella abortus phosphoglucomutase (pgm) mutant as a new live rough-phenotype vaccine. Infect Immun 71: 6264-6269.
5. Alton GG, Jones LM, Angus RD, Verger JM, (1988) Techniques for the brucellosis laboratory. Institut National de la Recherche Agronomique, Paris, France.
6. Foster G, Osterman BS, Godfroid J, Jaques I, Cloekaert A, (2007) Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int J Syst Evol Microbiol 57: 2688-2693.
7. Allen CA, Adams LG, Ficht TA, (1998) Transposon-derived Brucella abortus rough mutants are attenuated and exhibit reduced intracellular survival. Infect Immun 66: 1008-1016.
8. Braun W, (1947) Bacterial dissociation. Bacteriol Rev 11: 75-114.
9. Henry BS, (1933) Dissociation in genus Brucella. J Infect Dis 52: 374-402.
10. Schurig GG, Martin Roop R II, Bagchi T, Boyle S, Buhrman D, et al. (1991) Biological properties of RB51; a stable rough strain of Brucella abortus. Vet Microbiol 28: 171-188.
11. Moriyon IM, Grillo MJ, Monreal D, Gonzalez D, Marin C, et al. (2004) Rough vaccines in animal brucellosis: structural and genetic basis and present status. Vet Res 35: 1-38.
12. Schurig GG, Sriranganathan N, Corbel MJ, (2002) Brucellosis vaccines: past, present and future. Vet Microbiol 90: 479-496.
13. Adone R, Ciuchini F, Olsen SC, (2001) Field validation of RB51 antigen in a complement fixation test to identify calves vaccinated with Brucella abortus RB51. Clin Diagn Lab Immunol 8: 385-387.
14. Olsen SC, Bricker B, Palmer MV, Jensen AE, Cheville NF, (1999) Responses of cattle to two dosages of Brucella abortus strain RB51: serology, clearance and efficacy. Res Vet Sci 66: 101-105.
15. Adone R, Ciuchini F, (1999) Complement fixation test to assess humoral immunity in cattle and sheep vaccinated with Brucella abortus RB51. Clin Diagn Lab Immunol 6: 787-790.
16. Vemulapalli R, McQuiston JR, Schurig GG, Sriranganathan NM, Halling SM, (1999) Identification of and IS711 element interrupting the wboA gene of Brucella abortus vaccine strain RB51 and a PCR assay to distinguish strain RB51 from other Brucella species and strains, Clin Diagn Lab Immunol 6: 760-764.
17. McQuiston JR, Vemulapalli R, Inzana TJ, Schurig GG, Sriranganathan NM, et al. (1999) Genetic characterization of a Tn5-disrupted glycosyltransferase gene homologue in Brucella abortus and its effect on lipopolysaccharide composition and virulence. Infect Immun 67: 3830-3835.
18. Monreal D, Grillo MJ, Gonzalez D, Marin CM, de Miguel MJ, et al. (2003) Characterization of Brucella abortus O-Polysaccharide and core lipopolysaccharide mutants and demonstration that a complete core is required for rough vaccines to be efficient against Brucella abortus and Brucella ovis in the mouse model. Infect Immun 71: 3261-3271.
19. Vemulapalli R, He Y, Buccolo LS, Boyle SM, Sriranganathan NM, et al. (2000a) Complementation of Brucella abortus RB51 with a functional wboA gene results in O-antigen synthesis and enhanced vaccine efficacy but no change in rough phenotype and attenuation. Infect Immun 68: 3927-3932.
20. Vemulapalli R, He Y, Cravero S, Sriranganathan NM, Boyle SM, et al. (2000b) Overexpression of protective antigen as a novel approach to enhance vaccine efficacy of Brucella abortus strain RB51. Infect Immun 68: 3286-3289.
21. Cloeckaert A, de Vergifosse P, Dubray G, Limet JN, (1990) Identification of seven surface-exposed Brucella outer membrane proteins by use of monoclonal antibodies: immunogold labeling for electron microscopy and enzyme-linked immunosorbent assay. Infect Immun 58: 3980-3987.
22. Cloeckaert A, Weynants VE, Godfroid J, Verger JM, Grayon M, et al. (1998) O-Polysaccharide epitopic heterogeneity at the surface of Brucella spp. studied by enzyme-linked immunosorbent assay and flow cytometry. Clin Diagn Lab Immunol 5: 862-870.
23. Godfroid FB, Taminiau I, Danese P, Denoel A, Tibor V, et al. (1998) Identification of the perosamine synthetase gene of Brucella melitensis 16M and involvement of lipopolysaccharide O side chain in Brucella survival in mice and in macrophages. Infect Immun 66: 5485-93.
24. Godfroid F, Cloeckaert A, Taminiau B, Danese I, Tibor A, et al. (2000) Genetic organisation of the lypopolisaccharide O-antigen biosynthesis region of Brucella melitensis 16M (wbk). Res Microbiol 51: 655-668.
25. Cloeckaert A, Verger JM, Grayon M, Paquet JY, Garin-Bastuji B, et al. (2000) Conservation of seven genes involved in the biosynthesis of the lipopolysaccharide O-side chain in Brucella spp. Res Microbiol 151: 209-216.
26. González D, Grilló MJ, De Miguel MJ, Ali T, Arce-Gorvel V, et al. (2008) Brucellosis Vaccines: Assessment of Brucella melitensis Lipopolysaccharide Rough Mutants Defective in Core and O-Polysaccharide Synthesis and Export. PLoS ONE July 2008, 3: e2760.
27. Adone R, Francia M, Ciuchini F, (2008) Evaluation of Brucella melitensis B115 as rough-phenotype vaccine against B.melitensis and B.ovis infections. Vaccine 26: 4913-4917.
28. Adone R, Francia M, Pistoia C, Pesciaroli M, Pasquali P, (2011) B.melitensis rough strain B115 is protective against heterologous Brucella spp. infections. Vaccine doi:10.1016/j.vaccine.2011.01.072.
29. Adone R, Ciuchini F, Marianelli C, Tarantino M, Pistoia C, et al. (2005) Protective properties of rifampin-resistant rough mutants of Brucella melitensis. Infect Immun 73: 4198-4204.
30. Del Vecchio VG, Kapatral V, Redkar RJ, Patra G, Mujer C, et al. (2002) The genome sequence of the facultative intracellular pathogen Brucella melitensis, Proc Natl Acad Sci USA 99: 443-448.
31. Perry MB, Bundle DR, (1990) Advances in brucellosis research. pp. 76-88 Texas A&M University Press, College Station; 1990.
32. Gomes Cardoso P, Costa Macedo G, Azecedo V, Costa Olivera S, (2006) Brucella spp noncanonical LPS: structure, biosynthesis and interaction with host immune system. Microb Cell Fact 5: 13 doi:10.1186/1475-2859-5-13.
33. Zygmunt MS, Blasco JM, Letesson JJ, Cloeckaert A, Moriyon I, (2009) DNA polymorphism analysis of Brucella lipopolysaccharide genes reveals marked differences in O-polysaccharide biosynthetic genes between smooth and rough Brucella species and novel species-specific markers. BMC Microbiol 9: 92 doi:10.1186/1471-2180-9-92.
34. Vizcaíno N, Caro-Hernández P, Cloeckaert A, Fernández-Lago L, (2004) DNA polymorphism in the omp25/omp31 family of Brucella spp.: identification of a 1.7-kb inversion in Brucella cetaceae and of a 15.1-kb genomic island, absent from Brucella ovis, related to the synthesis of smooth lipopolysaccharide. Microbes Infect 6: 821-834.
35. Cloeckaert A, Zygmunt MS, Guilloteau LA, (2002) Brucella abortus vaccine strain RB51 produces low levels of M-like O-antigen, Vaccine 20: 1820-1822.
36. Winter AJ, Schurig GG, Boyle SM, Sriranganathan NM, Bevins JS, (1996) Protection of BALB/c mice against homologous and heterologous species of Brucella by rough strain vaccines derived from Brucella melitensis and Brucella suis biovar 4. Am J Vet Res 57: 677-683.
37. Jones LM, Diaz R, Taylor AG, (1973) Characterization of allergens prepared from smooth and rough strains of Brucella melitensis. Br J Exp Pathol 54: 492-508.
38. Marianelli C, Ciuchini F, Tarantino M, Pasquali P, Adone R, (2006) Molecular characterization of the rpoB gene in Brucella species: new potential molecular markers for genotyping. Microbes Infect 8: 860-865.
39. Cloeckaert A, Zygmunt MS, Nicolle JC, Dubray G, Limet JN, (1992) O-chain expression in the rough Brucella melitensis B115: induction of O-polysaccharide-specific monoclonal antibodies and intracellular localization demonstrated by immunoelectron microscopy. J Gen Microbiol 138: 1211-1219.
40. Cloeckaert A, Zygmunt MS, Dubray G, Limet JN, (1993) Characterization of O-polysaccharide specific monoclonal antibodies derived from mice infected with the rough Brucella melitensis strain B115. J Gen Microbiol 139: 1551-1556.
41. Halling SM, Detilleux PG, Tatum FM, Judge BA, Mayfield JE, (1991) Deletion of the BCSP31 gene of Brucella abortus by replacement. Infect Immun 59: 3863-3868.
42. Diaz R, Jones LM, Leong D, Wilson JB, (1968) Surface antigens of smooth Brucellae. J Bacteriol 96: 893-901.
43. Adone R, Francia M, Ciuchini F, (2008) Brucella melitensis B115-based complement fixation test to detect antibodies induced by Brucella rough strains. J Appl Microbiol 105: 567-574.