Nkx2.2: Cre knock-in mouse line: A novel tool for pancreas- and CNS-specific gene deletion

Genesis

Volumn 51, Issue 12, 2013, Pages 844-851

  Balderes, Dina A ^a   Magnuson, Mark A ^b   Sussel, Lori ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

CNS; Cre lox; Lineage; Nkx2.2; Pancreas

Indexed keywords

CRE RECOMBINASE; TRANSCRIPTION FACTOR NKX2.2;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL LINEAGE; CELL POPULATION; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; EMBRYO; ENZYME ACTIVITY; GENE DELETION; GENE EXPRESSION; GENE LOCUS; NKX2.2 CRE MOUSE; NONHUMAN; PANCREAS; PANCREAS CELL; PRIORITY JOURNAL; REPORTER GENE; TRANSGENIC MOUSE;

LYCOPERSICON ESCULENTUM;

CNS; CRE-LOX; LINEAGE; NKX2.2; PANCREAS;

ANIMALS; CENTRAL NERVOUS SYSTEM; GENE KNOCK-IN TECHNIQUES; HOMEODOMAIN PROTEINS; INTEGRASES; MICE; MICE, TRANSGENIC; ORGAN SPECIFICITY; PANCREAS; SEQUENCE DELETION; TRANSCRIPTION FACTORS;

EID: 84890800583     PISSN: 1526954X     EISSN: 1526968X     Source Type: Journal    
DOI: 10.1002/dvg.22715     Document Type: Article

References (88)

1. Arnes L, Leclerc K, Friel JM, Hipkens SB, Magnuson MA, Sussel L. 2012. Generation of Nkx2.2:lacZ mice using recombination-mediated cassette exchange technology. Genesis. DOI:10.1002/dvg.22037.
2. Briscoe J, Sussel L, Serup P, Hartigan-O'Connor D, Jessell TM, Rubenstein JL, Ericson J. 1999. Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signalling. Nature 398:622-627.
3. Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA. 2011. Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Dis Model Mech. DOI:dmm.006569 [pii] 10.1242/ dmm.006569.
4. Desai S, Loomis Z, Pugh-Bernard A, Schrunk J, Doyle MJ, Minic A, McCoy E, Sussel L. 2008. Nkx2.2 regulates cell fate choice in the enteroendocrine cell lineages of the intestine. Dev Biol 313:58-66.
5. Dymecki SM. 1996. Flp recombinase promotes sitespecific DNA recombination in embryonic stem cells and transgenic mice. Proc Natl Acad Sci USA 93:6191-6196.
6. Ericson J, Briscoe J, Rashbass P, van Heyningen V, Jessell TM. 1997a. Graded sonic hedgehog signaling and the specification of cell fate in the ventral neural tube. Cold Spring Harbor Symp Quant Biol 62:451-466.
7. Ericson J, Rashbass P, Schedl A, Brenner-Morton S, Kawakami A, van Heyningen V, Jessell TM, Briscoe J. 1997b. Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling. Cell 90:169-180.
8. Gu G, Brown JR, Melton DA. 2003. Direct lineage tracing reveals the ontogeny of pancreatic cell fates during mouse embryogenesis. Mech Dev 120:35-43.
9. Harno E, Cottrell EC, White A. 2013 Metabolic pitfalls of CNS cre-based technology. Cell Metab 18:21-28.
10. Jorgensen MC, Ahnfelt-Ronne J, Hald J, Madsen OD, Serup P, Hecksher-Sorensen J (2007) An illustrated review of early pancreas development in the mouse. Endocr Rev 28:685-705.
11. Lei Q, Jeong Y, Misra K, Li S, Zelman AK, Epstein DJ, Matise MP. 2006. Wnt signaling inhibitors regulate the transcriptional response to morphogenetic Shh-Gli signaling in the neural tube. Dev Cell 11:325-337.
12. Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H. 2010. A robust and highthroughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci 13: 133-140.
13. Magnuson MA, Osipovich AB. 2013. Pancreas-specificcre driver lines and considerations for their prudent use. Cell metabolism 18:9-20.
14. Mastracci TL, Wilcox CL, Arnes L, Panea C, Golden JA, May CL, Sussel L. 2011. Nkx2.2 and Arx genetically interact to regulate pancreatic endocrine cell developmen and endocrine hormone expression. Dev Biol 359:1-11.
15. Pattyn A, Vallstedt A, Dias JM, Samad OA, Krumlauf R, Rijli FM, Brunet JF, Ericson J. 2003a Coordinated temporal and spatial control of motor neuron and serotonergic neuron generation from a common pool of CNS progenitors. Genes Dev 17:729-737
16. Pattyn A, Vallstedt A, Dias JM, Sander M, Ericson J 2003b. Complementary roles for Nkx6 and Nkx2 class proteins in the establishment of motoneuron identity in the hindbrain. Development 130:4149-4159.
17. Prado CL, Pugh-Bernard AE, Elghazi L, Sosa-Pineda B, Sussel L. 2004. Ghrelin cells replace insulinproducing beta cells in two mouse models of pancreas development. Proc Natl Acad Sci USA 101: 2924-2929.
18. Sauer B. 1998. Inducible gene targeting in mice using the Cre/lox system. Methods 14:381-392.
19. Shimamura K, Hartigan DJ, Martinez S, Puelles L, Rubenstein JL. 1995. Longitudinal organization of the anterior neural plate and neural tube. Development 121:3923-3933.
20. Smedley D, Salimova E, Rosenthal N. 2011. Cre recombinase resources for conditional mouse mutagenesis. Methods 53:411-416.
21. Sussel L, Kalamaras J, Hartigan-O'Connor DJ, Meneses JJ, Pedersen RA, Rubenstein JL, German MS. 1998. Mice lacking the homeodomain transcription factor Nkx2.2 have diabetes due to arrested differentiation of pancreatic beta cells. Development 125:2213-2221.
22. Wang H, Lei Q, Oosterveen T, Ericson J, Matise MP. 2011. Tcf/Lef repressors differentially regulate Shh-Gli target gene activation thresholds to generate progenitor patterning in the developing CNS. Development 138:3711-3721.
23. Arnes L, Leclerc K, Friel JM, Hipkens SB, Magnuson MA, Sussel L. 2012. Generation of Nkx2.2:lacZ mice using recombination-mediated cassette exchange technology. Genesis. DOI:10.1002/dvg.22037.
24. Briscoe J, Sussel L, Serup P, Hartigan-O'Connor D, Jessell TM, Rubenstein JL, Ericson J. 1999. Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signalling. Nature 398:622-627.
25. Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA. 2011. Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Dis Model Mech. DOI:dmm.006569 [pii] 10.1242/ dmm.006569.
26. Desai S, Loomis Z, Pugh-Bernard A, Schrunk J, Doyle MJ, Minic A, McCoy E, Sussel L. 2008. Nkx2.2 regulates cell fate choice in the enteroendocrine cell lineages of the intestine. Dev Biol 313:58-66.
27. Dymecki SM. 1996. Flp recombinase promotes sitespecific DNA recombination in embryonic stem cells and transgenic mice. Proc Natl Acad Sci USA 93:6191-6196.
28. Ericson J, Briscoe J, Rashbass P, van Heyningen V, Jessell TM. 1997a. Graded sonic hedgehog signaling and the specification of cell fate in the ventral neural tube. Cold Spring Harbor Symp Quant Biol 62:451-466.
29. Ericson J, Rashbass P, Schedl A, Brenner-Morton S, Kawakami A, van Heyningen V, Jessell TM, Briscoe J. 1997b. Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling. Cell 90:169-180.
30. Gu G, Brown JR, Melton DA. 2003. Direct lineage tracing reveals the ontogeny of pancreatic cell fates during mouse embryogenesis. Mech Dev 120:35-43.
31. Harno E, Cottrell EC, White A. 2013 Metabolic pitfalls of CNS cre-based technology. Cell Metab 18:21-28.
32. Jorgensen MC, Ahnfelt-Ronne J, Hald J, Madsen OD, Serup P, Hecksher-Sorensen J (2007) An illustrated review of early pancreas development in the mouse. Endocr Rev 28:685-705.
33. Lei Q, Jeong Y, Misra K, Li S, Zelman AK, Epstein DJ, Matise MP. 2006. Wnt signaling inhibitors regulate the transcriptional response to morphogenetic Shh-Gli signaling in the neural tube. Dev Cell 11:325-337.
34. Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H. 2010. A robust and highthroughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci 13: 133-140.
35. Magnuson MA, Osipovich AB. 2013. Pancreas-specificcre driver lines and considerations for their prudent use. Cell metabolism 18:9-20.
36. Mastracci TL, Wilcox CL, Arnes L, Panea C, Golden JA, May CL, Sussel L. 2011. Nkx2.2 and Arx genetically interact to regulate pancreatic endocrine cell developmen and endocrine hormone expression. Dev Biol 359:1-11.
37. Pattyn A, Vallstedt A, Dias JM, Samad OA, Krumlauf R, Rijli FM, Brunet JF, Ericson J. 2003a Coordinated temporal and spatial control of motor neuron and serotonergic neuron generation from a common pool of CNS progenitors. Genes Dev 17:729-737
38. Pattyn A, Vallstedt A, Dias JM, Sander M, Ericson J 2003b. Complementary roles for Nkx6 and Nkx2 class proteins in the establishment of motoneuron identity in the hindbrain. Development 130:4149-4159.
39. Prado CL, Pugh-Bernard AE, Elghazi L, Sosa-Pineda B, Sussel L. 2004. Ghrelin cells replace insulinproducing beta cells in two mouse models of pancreas development. Proc Natl Acad Sci USA 101: 2924-2929.
40. Sauer B. 1998. Inducible gene targeting in mice using the Cre/lox system. Methods 14:381-392.
41. Shimamura K, Hartigan DJ, Martinez S, Puelles L, Rubenstein JL. 1995. Longitudinal organization of the anterior neural plate and neural tube. Development 121:3923-3933.
42. Smedley D, Salimova E, Rosenthal N. 2011. Cre recombinase resources for conditional mouse mutagenesis. Methods 53:411-416.
43. Sussel L, Kalamaras J, Hartigan-O'Connor DJ, Meneses JJ, Pedersen RA, Rubenstein JL, German MS. 1998. Mice lacking the homeodomain transcription factor Nkx2.2 have diabetes due to arrested differentiation of pancreatic beta cells. Development 125:2213-2221.
44. Wang H, Lei Q, Oosterveen T, Ericson J, Matise MP. 2011. Tcf/Lef repressors differentially regulate Shh-Gli target gene activation thresholds to generate progenitor patterning in the developing CNS. Development 138:3711-3721.
45. Arnes L, Leclerc K, Friel JM, Hipkens SB, Magnuson MA, Sussel L. 2012. Generation of Nkx2.2:lacZ mice using recombination-mediated cassette exchange technology. Genesis. DOI:10.1002/dvg.22037.
46. Briscoe J, Sussel L, Serup P, Hartigan-O'Connor D, Jessell TM, Rubenstein JL, Ericson J. 1999. Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signalling. Nature 398:622-627.
47. Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA. 2011. Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Dis Model Mech. DOI:dmm.006569 [pii] 10.1242/ dmm.006569.
48. Desai S, Loomis Z, Pugh-Bernard A, Schrunk J, Doyle MJ, Minic A, McCoy E, Sussel L. 2008. Nkx2.2 regulates cell fate choice in the enteroendocrine cell lineages of the intestine. Dev Biol 313:58-66.
49. Dymecki SM. 1996. Flp recombinase promotes sitespecific DNA recombination in embryonic stem cells and transgenic mice. Proc Natl Acad Sci USA 93:6191-6196.
50. Ericson J, Briscoe J, Rashbass P, van Heyningen V, Jessell TM. 1997a. Graded sonic hedgehog signaling and the specification of cell fate in the ventral neural tube. Cold Spring Harbor Symp Quant Biol 62:451-466.
51. Ericson J, Rashbass P, Schedl A, Brenner-Morton S, Kawakami A, van Heyningen V, Jessell TM, Briscoe J. 1997b. Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling. Cell 90:169-180.
52. Gu G, Brown JR, Melton DA. 2003. Direct lineage tracing reveals the ontogeny of pancreatic cell fates during mouse embryogenesis. Mech Dev 120:35-43.
53. Harno E, Cottrell EC, White A. 2013 Metabolic pitfalls of CNS cre-based technology. Cell Metab 18:21-28.
54. Jorgensen MC, Ahnfelt-Ronne J, Hald J, Madsen OD, Serup P, Hecksher-Sorensen J (2007) An illustrated review of early pancreas development in the mouse. Endocr Rev 28:685-705.
55. Lei Q, Jeong Y, Misra K, Li S, Zelman AK, Epstein DJ, Matise MP. 2006. Wnt signaling inhibitors regulate the transcriptional response to morphogenetic Shh-Gli signaling in the neural tube. Dev Cell 11:325-337.
56. Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H. 2010. A robust and highthroughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci 13: 133-140.
57. Magnuson MA, Osipovich AB. 2013. Pancreas-specificcre driver lines and considerations for their prudent use. Cell metabolism 18:9-20.
58. Mastracci TL, Wilcox CL, Arnes L, Panea C, Golden JA, May CL, Sussel L. 2011. Nkx2.2 and Arx genetically interact to regulate pancreatic endocrine cell developmen and endocrine hormone expression. Dev Biol 359:1-11.
59. Pattyn A, Vallstedt A, Dias JM, Samad OA, Krumlauf R, Rijli FM, Brunet JF, Ericson J. 2003a Coordinated temporal and spatial control of motor neuron and serotonergic neuron generation from a common pool of CNS progenitors. Genes Dev 17:729-737
60. Pattyn A, Vallstedt A, Dias JM, Sander M, Ericson J 2003b. Complementary roles for Nkx6 and Nkx2 class proteins in the establishment of motoneuron identity in the hindbrain. Development 130:4149-4159.
61. Prado CL, Pugh-Bernard AE, Elghazi L, Sosa-Pineda B, Sussel L. 2004. Ghrelin cells replace insulinproducing beta cells in two mouse models of pancreas development. Proc Natl Acad Sci USA 101: 2924-2929.
62. Sauer B. 1998. Inducible gene targeting in mice using the Cre/lox system. Methods 14:381-392.
63. Shimamura K, Hartigan DJ, Martinez S, Puelles L, Rubenstein JL. 1995. Longitudinal organization of the anterior neural plate and neural tube. Development 121:3923-3933.
64. Smedley D, Salimova E, Rosenthal N. 2011. Cre recombinase resources for conditional mouse mutagenesis. Methods 53:411-416.
65. Sussel L, Kalamaras J, Hartigan-O'Connor DJ, Meneses JJ, Pedersen RA, Rubenstein JL, German MS. 1998. Mice lacking the homeodomain transcription factor Nkx2.2 have diabetes due to arrested differentiation of pancreatic beta cells. Development 125:2213-2221.
66. Wang H, Lei Q, Oosterveen T, Ericson J, Matise MP. 2011. Tcf/Lef repressors differentially regulate Shh-Gli target gene activation thresholds to generate progenitor patterning in the developing CNS. Development 138:3711-3721.
67. Arnes L, Leclerc K, Friel JM, Hipkens SB, Magnuson MA, Sussel L. 2012. Generation of Nkx2.2:lacZ mice using recombination-mediated cassette exchange technology. Genesis. DOI:10.1002/dvg.22037.
68. Briscoe J, Sussel L, Serup P, Hartigan-O'Connor D, Jessell TM, Rubenstein JL, Ericson J. 1999. Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signalling. Nature 398:622-627.
69. Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA. 2011. Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Dis Model Mech. DOI:dmm.006569 [pii] 10.1242/ dmm.006569.
70. Desai S, Loomis Z, Pugh-Bernard A, Schrunk J, Doyle MJ, Minic A, McCoy E, Sussel L. 2008. Nkx2.2 regulates cell fate choice in the enteroendocrine cell lineages of the intestine. Dev Biol 313:58-66.
71. Dymecki SM. 1996. Flp recombinase promotes sitespecific DNA recombination in embryonic stem cells and transgenic mice. Proc Natl Acad Sci USA 93:6191-6196.
72. Ericson J, Briscoe J, Rashbass P, van Heyningen V, Jessell TM. 1997a. Graded sonic hedgehog signaling and the specification of cell fate in the ventral neural tube. Cold Spring Harbor Symp Quant Biol 62:451-466.
73. Ericson J, Rashbass P, Schedl A, Brenner-Morton S, Kawakami A, van Heyningen V, Jessell TM, Briscoe J. 1997b. Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling. Cell 90:169-180.
74. Gu G, Brown JR, Melton DA. 2003. Direct lineage tracing reveals the ontogeny of pancreatic cell fates during mouse embryogenesis. Mech Dev 120:35-43.
75. Harno E, Cottrell EC, White A. 2013 Metabolic pitfalls of CNS cre-based technology. Cell Metab 18:21-28.
76. Jorgensen MC, Ahnfelt-Ronne J, Hald J, Madsen OD, Serup P, Hecksher-Sorensen J (2007) An illustrated review of early pancreas development in the mouse. Endocr Rev 28:685-705.
77. Lei Q, Jeong Y, Misra K, Li S, Zelman AK, Epstein DJ, Matise MP. 2006. Wnt signaling inhibitors regulate the transcriptional response to morphogenetic Shh-Gli signaling in the neural tube. Dev Cell 11:325-337.
78. Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H. 2010. A robust and highthroughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci 13: 133-140.
79. Magnuson MA, Osipovich AB. 2013. Pancreas-specificcre driver lines and considerations for their prudent use. Cell metabolism 18:9-20.
80. Mastracci TL, Wilcox CL, Arnes L, Panea C, Golden JA, May CL, Sussel L. 2011. Nkx2.2 and Arx genetically interact to regulate pancreatic endocrine cell developmen and endocrine hormone expression. Dev Biol 359:1-11.
81. Pattyn A, Vallstedt A, Dias JM, Samad OA, Krumlauf R, Rijli FM, Brunet JF, Ericson J. 2003a Coordinated temporal and spatial control of motor neuron and serotonergic neuron generation from a common pool of CNS progenitors. Genes Dev 17:729-737
82. Pattyn A, Vallstedt A, Dias JM, Sander M, Ericson J 2003b. Complementary roles for Nkx6 and Nkx2 class proteins in the establishment of motoneuron identity in the hindbrain. Development 130:4149-4159.
83. Prado CL, Pugh-Bernard AE, Elghazi L, Sosa-Pineda B, Sussel L. 2004. Ghrelin cells replace insulinproducing beta cells in two mouse models of pancreas development. Proc Natl Acad Sci USA 101: 2924-2929.
84. Sauer B. 1998. Inducible gene targeting in mice using the Cre/lox system. Methods 14:381-392.
85. Shimamura K, Hartigan DJ, Martinez S, Puelles L, Rubenstein JL. 1995. Longitudinal organization of the anterior neural plate and neural tube. Development 121:3923-3933.
86. Smedley D, Salimova E, Rosenthal N. 2011. Cre recombinase resources for conditional mouse mutagenesis. Methods 53:411-416.
87. Sussel L, Kalamaras J, Hartigan-O'Connor DJ, Meneses JJ, Pedersen RA, Rubenstein JL, German MS. 1998. Mice lacking the homeodomain transcription factor Nkx2.2 have diabetes due to arrested differentiation of pancreatic beta cells. Development 125:2213-2221.
88. Wang H, Lei Q, Oosterveen T, Ericson J, Matise MP. 2011. Tcf/Lef repressors differentially regulate Shh-Gli target gene activation thresholds to generate progenitor patterning in the developing CNS. Development 138:3711-3721.