Lactate down-regulates matrix systhesis and promotes apoptosis and autophagy in rat nucleus pulposus cells

Journal of Orthopaedic Research

Volumn 32, Issue 2, 2014, Pages 253-261

  Wu, Wei ^a   Zhang, Xiaolei ^a,b   Hu, Xuqi ^a   Wang, Xiangyang ^a   Sun, Liaojun ^a   Zheng, Xuhao ^a   Jiang, Libo ^a   Ni, Xiao ^a   Xu, Cong ^a   Tian, Naifeng ^a   Zhu, Siping ^a   Xu, Huazi ^a  

^a THE SECOND AFFILIATED HOSPITAL AND YUYING CHILDREN S HOSPITAL OF WENZHOU MEDICAL UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

apoptosis; autophagy; cell death; intervertebral disk degeneration; lactate

Indexed keywords

BECLIN 1; COLLAGEN TYPE 1; COLLAGEN TYPE 2; LACTIC ACID; MESSENGER RNA; PROTEIN P62;

ANIMAL EXPERIMENT; APOPTOSIS; ARTICLE; AUTOPHAGY; CELL NUCLEUS; CELL PROLIFERATION; CELL VIABILITY; DOWN REGULATION; FEMALE; GENE EXPRESSION; LIGHT CHAIN; MALE; NONHUMAN; NUCLEUS PULPOSUS; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

APOPTOSIS; AUTOPHAGY; CELL DEATH; INTERVERTEBRAL DISK DEGENERATION; LACTATE;

ANIMALS; APOPTOSIS; AUTOPHAGY; CELL PROLIFERATION; CELL SURVIVAL; DOWN-REGULATION; FEMALE; GLYCOSAMINOGLYCANS; INTERVERTEBRAL DISC; INTERVERTEBRAL DISC DEGENERATION; LACTIC ACID; MALE; RATS; RATS, SPRAGUE-DAWLEY;

EID: 84890553057     PISSN: 07360266     EISSN: 1554527X     Source Type: Journal    
DOI: 10.1002/jor.22503     Document Type: Article

References (41)

1. Nishida K, Kang JD, Suh J-K, et al. 1998. Adenovirus-mediated gene transfer to nucleus pulposus cells: implications for the treatment of intervertebral disc degeneration. Spine 23: 2437-2442.
2. Sive J, Baird P, Jeziorsk M, et al. 2002. Expression of chondrocyte markers by cells of normal and degenerate intervertebral discs. Mol Pathol 55: 91-97.
3. Eyre DR, Muir H., 1976. Types I and II collagens in intervertebral disc. Interchanging radial distributions in annulus fibrosus. Biochem J 157: 267.
4. Jöud A, Petersson IF, Englund M., 2012. Low back pain: epidemiology of consultations. Arthritis Care Res 64: 1084-1088.
5. Luoma K, Riihimäki H, Luukkonen R, et al. 2000. Low back pain in relation to lumbar disc degeneration. Spine 25: 487-492.
6. Bartels EM, Fairbank JC, Winlove CP, et al. 1998. Oxygen and lactate concentrations measured in vivo in the intervertebral discs of patients with scoliosis and back pain. Spine 23: 1-7.
7. Bibby SR, Jones DA, Ripley RM, et al. 2005. Metabolism of the intervertebral disc: effects of low levels of oxygen, glucose, and pH on rates of energy metabolism of bovine nucleus pulposus cells. Spine 30: 487-496.
8. Hughes S, Freemont A, Hukins D, et al. 2012. The pathogenesis of degeneration of the intervertebral disc and emerging therapies in the management of back pain. J Bone Joint Surg Br 94: 1298-1304.
9. Vonk LA, Kroeze RJ, Doulabi BZ, et al. 2010. Caprine articular, meniscus and intervertebral disc cartilage: an integral analysis of collagen network and chondrocytes. Matrix Biol 29: 209-218.
10. Cs-Szabo G, Ragasa-San Juan D, Turumella V, et al. 2002. Changes in mRNA and protein levels of proteoglycans of the anulus fibrosus and nucleus pulposus during intervertebral disc degeneration. Spine 27: 2212-2219.
11. Ohshima H, Urban J., 1992. The effect of lactate and pH on proteoglycan and protein synthesis rates in the intervertebral disc. Spine 17: 1079-1082.
12. Holm S, Nachemson A., 1988. Nutrition of the intervertebral disc: acute effects of cigarette smoking: an experimental animal study. Ups J Med Sci 93: 91-99.
13. Zhao C-Q, Jiang L-S, Dai L-Y., 2006. Programmed cell death in intervertebral disc degeneration. Apoptosis 11: 2079-2088.
14. Jiang L, Zhang X, Zheng X, et al. 2012. Apoptosis, senescence, and autophagy in rat nucleus pulposus cells: implications for diabetic intervertebral disc degeneration. J Orthop Res 31: 692-702.
15. Bursch W, Ellinger A, Gerner C, et al. 2000. Programmed cell death (PCD): apoptosis, autophagic PCD, or others ? Ann N Y Acad Sci 926: 1-12.
16. Kerr JF, Wyllie AH, Currie AR., 1972. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26: 239.
17. Slee EA, Adrain C, Martin SJ., 2001. Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demolition phase of apoptosis. J Biol Chem 276: 7320-7326.
18. Bustamante-Marín X, Quiroga C, Lavandero S, et al. 2012. Apoptosis, necrosis and autophagy are influenced by metabolic energy sources in cultured rat spermatocytes. Apoptosis 17: 539-550.
19. Budihardjo I, Oliver H, Lutter M, et al. 1999. Biochemical pathways of caspase activation during apoptosis. Annu Rev Cell Dev Biol 15: 269-290.
20. Mehrpour M, Esclatine A, Beau I, et al. 2010. Overview of macroautophagy regulation in mammalian cells. Cell Res 20: 748-762.
21. Klionsky DJ, Emr SD., 2000. Autophagy as a regulated pathway of cellular degradation. Science 290: 1717-1721.
22. Shen C, Yan J, Jiang L-S, et al. 2011. Autophagy in rat annulus fibrosus cells: evidence and possible implications. Arthritis Res Ther 13: R132.
23. Yang Z, Klionsky DJ., 2009. An overview of the molecular mechanism of autophagy. In: Autophagy in infection and immunity. Curr Top Microbiol Immunol 335: 1-32.
24. Tanida I, Minematsu-Ikeguchi N, Ueno T, et al. 2005. Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy 1: 84-91.
25. Liang XH, Jackson S, Seaman M, et al. 1999. Induction of autophagy and inhibition of tumorigenesis by beclin 1. Nature 402: 672-676.
26. Pattingre S, Tassa A, Qu X, et al. 2005. Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy. Cell 122: 927-940.
27. Farndale RW, Buttle DJ, Barrett AJ., 1986. Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta 883: 173-177.
28. Livak KJ, Schmittgen TD., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25: 402-408.
29. Holm S, Urban J., 1987. The intervertebral disc: factors contributing to its nutrition and matrix turnover. Joint loading: biology and health of articular structures. Bristol, United Kingdom: John Wright and Sons Ltd. p 187-226.
30. Urban J, Holm S, Maroudas A, et al. 1982. Nutrition of the intervertebral disc: effect of fluid flow on solute transport. Clin Orthop 170: 296-302.
31. Urban JP, Smith S, Fairbank JC., 2004. Nutrition of the intervertebral disc. Spine (Phila, PA) 29: 2700-2709.
32. Risbud MV, Guttapalli A, Stokes DG, et al. 2006. Nucleus pulposus cells express HIF-1α under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment. J Cell Biochem 98: 152-159.
33. Holm S, Maroudas A, Urban J, et al. 1981. Nutrition of the intervertebral disc: solute transport and metabolism. Connect Tissue Res 8: 101-119.
34. Ishihara H, Urban JP., 1999. Effects of low oxygen concentrations and metabolic inhibitors on proteoglycan and protein synthesis rates in the intervertebral disc. J Orthop Res 17: 829-835.
35. Van Der Werf M, Lezuo P, Maissen O, et al. 2007. Inhibition of vertebral endplate perfusion results in decreased intervertebral disc intranuclear diffusive transport. J Anat 211: 769-774.
36. Mokhbi Soukane D, Shirazi-Adl A, Urban J., 2007. Computation of coupled diffusion of oxygen, glucose and lactic acid in an intervertebral disc. J Biomech 40: 2645-2654.
37. Grunhagen T, Wilde G, Soukane DM, et al. 2006. Nutrient supply and intervertebral disc metabolism. J Bone Joint Surg 88: 30-35.
38. Maiuri MC, Criollo A, Tasdemir E, et al. 2007. BH3-only proteins and BH3 mimetics induce autophagy by competitively disrupting the interaction between Beclin 1 and Bcl-2/Bcl-XL. Autophagy 3: 374-376.
39. Mizushima N, Yoshimori T., 2007. How to interpret LC3 immunoblotting. Autophagy 3: 542-545.
40. Ye W, Xu K, Huang D, et al. 2011. Age-related increases of macroautophagy and chaperone-mediated autophagy in rat nucleus pulposus. Connect Tissue Res 52: 472-478.
41. Meredith D, Bell P, McClure B, et al. 2002. Functional and molecular characterisation of lactic acid transport in bovine articular chondrocytes. Cell Physiol Biochem 12: 227-234.