Production of barley endoprotease B2 in Pichia pastoris and its proteolytic activity against native and recombinant hordeins

Phytochemistry

Volumn 97, Issue , 2014, Pages 11-19

  Rosenkilde, Anne Lind ^a   Dionisio, Giuseppe ^a   Holm, Preben B ^a   Brinch Pedersen, Henrik ^a  

^a AARHUS UNIVERSITY   (Denmark)

Author keywords

Heterologous expression; Hordein degradation; Hordeum vulgare; Hordeum vulgare endoprotease B2; Pichia pastoris; Proteolytic resistant tag

Indexed keywords

ESCHERICHIA COLI; HORDEUM; HORDEUM VULGARE; HORDEUM VULGARE SUBSP. VULGARE; PICHIA PASTORIS;

CYSTEINE PROTEINASE; DITHIOTHREITOL; GLUTEN; PROTEINASE; RECOMBINANT PROTEIN; THIOREDOXIN;

ARTICLE; BARLEY; ENDO H; ENDO-Β-N-ACETYLGLUCOSAMINIDASE H; ENZYMOLOGY; FERMENTATION; GENETICS; HETEROLOGOUS EXPRESSION; HORDEIN DEGRADATION; HORDEUM; HORDEUM VULGARE ENDOPROTEASE B1; HORDEUM VULGARE ENDOPROTEASE B2; HORDEUM VULGARE ENDOPROTEASEB2; HVEPB1; HVEPB2; METABOLISM; PEPTIDE MAPPING; PICHIA; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEOLYTIC RESISTANT TAG; TRX;

DITHIOTHREITOL; DTT; ENDO H; ENDO-Β-N-ACETYLGLUCOSAMINIDASE H; HETEROLOGOUS EXPRESSION; HORDEIN DEGRADATION; HORDEUM VULGARE; HORDEUM VULGARE ENDOPROTEASE B1; HORDEUM VULGARE ENDOPROTEASE B2; HORDEUM VULGARE ENDOPROTEASEB2; HVEPB1; HVEPB2; PICHIA PASTORIS; PROTEOLYTIC RESISTANT TAG; THIOREDOXIN; TRX;

CYSTEINE PROTEASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; FERMENTATION; GLUTENS; HORDEUM; PEPTIDE MAPPING; PICHIA; RECOMBINANT PROTEINS;

EID: 84890350323     PISSN: 00319422     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.phytochem.2013.09.004     Document Type: Article

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