Glycosylations and truncations of functional cereal phytases expressed and secreted by Pichia pastoris documented by mass spectrometry

Protein Expression and Purification

Volumn 82, Issue 1, 2012, Pages 179-185

  Dionisio, Giuseppe ^a   Jørgensen, Malene ^b,c   Welinder, Karen Gjesing ^b   Brinch Pedersen, Henrik ^a  

^a AARHUS UNIVERSITY   (Denmark)

^b AALBORG UNIVERSITY   (Denmark)

^c AALBORG UNIVERSITY HOSPITAL   (Denmark)

Author keywords

Mass spectrometry of hyper glycosylated peptides; Pichia pastoris secreted protein; Posttranslational modification in Pichia; Recombinant phytase; Wheat phytase

Indexed keywords

PHYTASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CEREAL; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; GLYCOSYLATION; HORDEUM; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; PICHIA; PROTEIN PROCESSING; SEQUENCE ALIGNMENT; WHEAT;

6-PHYTASE; AMINO ACID SEQUENCE; CEREALS; GENE EXPRESSION; GLYCOSYLATION; HORDEUM; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PICHIA; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; TRITICUM;

EID: 84857330031     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.12.003     Document Type: Article

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