Phosphorylation of the Rab exchange factor Sec2p directs a switch in regulatory binding partners

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 50, 2013, Pages 19995-20002

  Stalder, Danièle ^a   Mizuno Yamasaki, Emi ^a,c   Ghassemian, Majid ^b   Novick, Peter J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c GUNMA UNIVERSITY   (Japan)

Author keywords

Membrane traffic; Phospho regulation; Vesicle maturation; Yeast

Indexed keywords

EXOCYST; FUNGAL PROTEIN; GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANOSINE TRIPHOSPHATE; PHOSPHATIDYLINOSITOL 4 PHOSPHATE; RAB PROTEIN; SEC15P PROTEIN; SEC2P PROTEIN; SEC4P PROTEIN; UNCLASSIFIED DRUG; YPT32P PROTEIN;

ARTICLE; BINDING SITE; CELL FUSION; CELL MEMBRANE; CELL MEMBRANE TRANSPORT; CONTROLLED STUDY; GOLGI COMPLEX; INTRACELLULAR SIGNALING; NONHUMAN; POSITIVE FEEDBACK; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; SECRETORY PATHWAY; SECRETORY VESICLE;

EID: 84890284248     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1320029110     Document Type: Article

References (23)

1. Hutagalung AH, Novick PJ (2011) Role of Rab GTPases in membrane traffic and cell physiology. Physiol Rev 91(1):119-149.
2. Cherfils J, Zeghouf M (2013) Regulation of small GTPases by GEFs, GAPs, and GDIs. Physiol Rev 93(1):269-309.
3. Jin Y, et al. (2011) Myosin V transports secretory vesicles via a Rab GTPase cascade and interaction with the exocyst complex. Dev Cell 21(6):1156-1170.
4. Guo W, Roth D, Walch-Solimena C, Novick P (1999) The exocyst is an effector for Sec4p, targeting secretory vesicles to sites of exocytosis. EMBO J 18(4):1071-1080.
5. Grosshans BL, et al. (2006) The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p. J Cell Biol 172(1):55-66.
6. Walch-Solimena C, Collins RN, Novick PJ (1997) Sec2p mediates nucleotide exchange on Sec4p and is involved in polarized delivery of post-Golgi vesicles. J Cell Biol 137(7):1495-1509.
7. Dong G, Medkova M, Novick P, Reinisch KM (2007) A catalytic coiled coil: Structural insights into the activation of the Rab GTPase Sec4p by Sec2p. Mol Cell 25(3):455-462.
8. Elkind NB, Walch-Solimena C, Novick PJ (2000) The role of the COOH terminus of Sec2p in the transport of post-Golgi vesicles. J Cell Biol 149(1):95-110.
9. Ortiz D, Medkova M, Walch-Solimena C, Novick P (2002) Ypt32 recruits the Sec4p guanine nucleotide exchange factor, Sec2p, to secretory vesicles; evidence for a Rab cascade in yeast. J Cell Biol 157(6):1005-1015.
10. Knödler A, et al. (2010) Coordination of Rab8 and Rab11 in primary ciliogenesis. Proc Natl Acad Sci USA 107(14):6346-6351.
11. Mizuno-Yamasaki E, Rivera-Molina F, Novick P (2012) GTPase networks in membrane traffic. Annu Rev Biochem 81:637-659.
12. Pusapati GV, Luchetti G, Pfeffer SR (2012) Ric1-Rgp1 complex is a guanine nucleotide exchange factor for the late Golgi Rab6A GTPase and an effector of the medial Golgi Rab33B GTPase. J Biol Chem 287(50):42129-42137.
13. Nottingham RM, et al. (2012) RUTBC2 protein, a Rab9A effector and GTPase-activating protein for Rab36. J Biol Chem 287(27):22740-22748.
14. Medkova M, France YE, Coleman J, Novick P (2006) The rab exchange factor Sec2p reversibly associates with the exocyst. Mol Biol Cell 17(6):2757-2769.
15. Mizuno-Yamasaki E, Medkova M, Coleman J, Novick P (2010) Phosphatidylinositol 4-phosphate controls both membrane recruitment and a regulatory switch of the Rab GEF Sec2p. Dev Cell 18(5):828-840.
16. Chiba S, Amagai Y, Homma Y, Fukuda M, Mizuno K (2013) NDR2-mediated Rabin8 phosphorylation is crucial for ciliogenesis by switching binding specificity from phosphatidylserine to Sec15. EMBO J 32(6):874-885.
17. Ultanir SK, et al. (2012) Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development. Neuron 73(6):1127-1142.
18. Jedd G, Mulholland J, Segev N (1997) Two new Ypt GTPases are required for exit from the yeast. J Cell Biol 137(3):563-580.
19. Cabrera M, et al. (2010) Phosphorylation of a membrane curvature-sensing motif switches function of the HOPS subunit Vps41 in membrane tethering. J Cell Biol 191(4):845-859.
20. Kurischko C, et al. (2008) The yeast LATS/Ndr kinase Cbk1 regulates growth via Golgi-dependent glycosylation and secretion. Mol Biol Cell 19(12):5559-5578.
21. Bishop A, et al. (2010) Hyphal growth in Candida albicans requires the phosphorylation of Sec2 by the Cdc28-Ccn1/Hgc1 kinase. EMBO J 29(17):2930-2942.
22. Chen S, Novick P, Ferro-Novick S (2012) ER network formation requires a balance of the dynamin-like GTPase Sey1p and the Lunapark family member Lnp1p. Nat Cell Biol 14(7):707-716.
23. Shevchenko A, Wilm M, Vorm O, Mann M (1996) Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 68(5):850-858.