Analytical characterization of a monoclonal antibody therapeutic reveals a three-light chain species that is efficiently removed using hydrophobic interaction chromatography

mAbs

Volumn 5, Issue 6, 2013, Pages 925-935

  Wollacott, Rachel B ^a   Casaz, Paul L ^a   Morin, Trevor J ^a,b   Zhu, H Lily ^a,c   Anderson, Roger S ^a   Babcock, Gregory J ^a,d   Que, John ^a,e   Thomas Jr , William D ^a   Ozturk, Sadettin S ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^b Two Pore Guys Inc   (United States)

^c BIOGEN   (United States)

^d VISTERRA INC   (United States)

^e NONE

Author keywords

Antibody size variant; Design of experiments; Hydrophobic interaction chromatography; Monoclonal antibody; Size exclusion chromatography; Third light chain

Indexed keywords

DIMER; HUMAN MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY; MONOMER; PROTEIN A; RESIN; IMMUNOGLOBULIN LIGHT CHAIN;

ANIMAL CELL; ANION EXCHANGE; ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; CARBOXY TERMINAL SEQUENCE; CATION EXCHANGE; CHO CELL; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; ENZYME LINKED IMMUNOSORBENT ASSAY; FAST PROTEIN LIQUID CHROMATOGRAPHY; FLOW RATE; GEL ELECTROPHORESIS; GEL PERMEATION CHROMATOGRAPHY; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; HYDROPHOBICITY; LIGHT CHAIN; MOLECULAR WEIGHT; NONHUMAN; OXIDATION; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PURIFICATION; PURIFICATION; ANIMAL; CELL CULTURE; CHEMICAL PHENOMENA; CHEMISTRY; CHO CELL LINE; CRICETULUS; HAMSTER; HUMAN;

ANIMALS; ANTIBODIES, MONOCLONAL; CELLS, CULTURED; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; CRICETULUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; IMMUNOGLOBULIN LIGHT CHAINS; MOLECULAR WEIGHT;

EID: 84889818943     PISSN: 19420862     EISSN: 19420870     Source Type: Journal    
DOI: 10.4161/mabs.26192     Document Type: Article

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