Triple light chain antibodies: Factors that influence its formation in cell culture

Biotechnology and Bioengineering

Volumn 105, Issue 4, 2010, Pages 748-760

  Gomez, Natalia ^a   Vinson, Abigail R ^a   Ouyang, Jun ^a   Nguyen, Mary D H ^a   Chen, Xiaoying Nancy ^a   Sharma, Vikas K ^a   Yuk, Inn H ^a  

^a GENENTECH INC   (United States)

Author keywords

CHO cells; Glutathione; Light chain; THIOMAB

Indexed keywords

AMINO ACID SEQUENCE; CELL LINES; CHINESE HAMSTER OVARY CELLS; CHO CELL; DISULFIDE BONDS; EXTRACELLULAR; FREE LIGHT CHAINS; GLUTATHIONES; HEAVY CHAIN; IN-CELL; LIGHT CHAIN; MICROCHIP ELECTROPHORESIS; NEW SPECIES; REACTIVE CYSTEINE; RECOMBINANT ANTIBODY; SPECIFIC PRODUCTION RATE; STABLE CELL LINES;

AMINO ACIDS; ANTIBODIES; CELLS; CHROMATOGRAPHIC ANALYSIS; ELECTROPHORESIS; ENCODING (SYMBOLS); GENE EXPRESSION; MASS SPECTROMETRY; ORGANIC ACIDS; ORGANIC POLYMERS; POLYPEPTIDES; SIZE EXCLUSION CHROMATOGRAPHY;

CELL CULTURE;

CYSTEINE; GLUTATHIONE; MESSENGER RNA; POLYPEPTIDE; RECOMBINANT ANTIBODY; RECOMBINANT ANTIBODY 3LC; RECOMBINANT ANTIBODY THIOMAB; UNCLASSIFIED DRUG; ANTIBODY; IMMUNOGLOBULIN LIGHT CHAIN; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CELL CULTURE; CONTROLLED STUDY; DISULFIDE BOND; GEL PERMEATION CHROMATOGRAPHY; HEAVY CHAIN; HYDROPHILICITY; LIGHT CHAIN; MASS SPECTROMETRY; MICROCHIP ELECTROPHORESIS; PROTEIN EXPRESSION; ANIMAL; CHO CELL; CRICETULUS; CULTURE TECHNIQUE; GENETICS; HAMSTER; METABOLISM; PROTEIN ENGINEERING;

CRICETULUS GRISEUS;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; CYSTEINE; GLUTATHIONE; IMMUNOGLOBULIN LIGHT CHAINS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; RNA, MESSENGER;

EID: 77449117737     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22580     Document Type: Article

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