Transcriptional regulation improves the throughput of three-hybrid counter selections in Saccharomyces cerevisiae

Biotechnology Journal

Volumn 8, Issue 12, 2013, Pages 1485-1491

  Harton, Marie D ^a   Wingler, Laura M ^a,b   Cornish, Virginia W ^a  

^a Columbia University ^*  (United States)

^b DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Dual tetracycline system; High throughput; In vivo assay; Synthetic biology; Yeast three hybrid assay

Indexed keywords

DUAL TETRACYCLINE SYSTEM; HIGH-THROUGHPUT; IN-VIVO; SYNTHETIC BIOLOGY; YEAST THREE-HYBRID;

BIOCHEMICAL ENGINEERING; CHEMICAL ACTIVATION; GENES; GENETIC ENGINEERING; LIBRARIES; SYNTHESIS (CHEMICAL);

YEAST;

TETRACYCLINE;

ARTICLE; CONTROLLED STUDY; DNA BINDING; DOWNSTREAM PROCESSING; GENETIC PROCEDURES; GENETIC SELECTION; HYBRID GENE; NONHUMAN; PRIORITY JOURNAL; REPORTER GENE; SACCHAROMYCES CEREVISIAE; THREE HYBRID SYSTEM; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

SACCHAROMYCES CEREVISIAE;

DUAL TETRACYCLINE SYSTEM; HIGH-THROUGHPUT; IN VIVO ASSAY; SYNTHETIC BIOLOGY; YEAST THREE-HYBRID ASSAY;

GENE EXPRESSION REGULATION, FUNGAL; GENES, REPORTER; HIGH-THROUGHPUT SCREENING ASSAYS; SACCHAROMYCES CEREVISIAE; SYNTHETIC BIOLOGY; TETRACYCLINE; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 84889672397     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201300186     Document Type: Article

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