Interaction between human Ctf4 and the Cdc45/Mcm2-7/GINS (CMG) replicative helicase

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 49, 2013, Pages 19760-19765

  Kang, Young Hoon ^a   Farina, Andrea ^a   Bermudez, Vladimir P ^a   Tappin, Inger ^a   Du, Fang ^a   Galal, Wiebke Chemnitz ^a   Hurwitz, Jerard ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

Dimerization; Replication initiation; Replisome core structure

Indexed keywords

CELL CYCLE PROTEIN 45; CHROMOSOME TRANSMISSION FIDELITY 4; HELICASE; MINICHROMOSOME MAINTENANCE PROTEIN 2; PROTEIN GINS; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; DNA BINDING; DNA DENATURATION; DNA REPLICATION; ENZYME ACTIVITY; HELA CELL; HUMAN; HYDRODYNAMICS; IMMUNOPRECIPITATION; IN VITRO STUDY; INSECT CELL; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; REPLISOME; SPODOPTERA FRUGIPERDA; STOICHIOMETRY;

DIMERIZATION; REPLICATION INITIATION; REPLISOME CORE STRUCTURE;

ANIMALS; BLOTTING, WESTERN; CELL CYCLE PROTEINS; DENSITOMETRY; DIMERIZATION; DNA HELICASES; DNA PRIMERS; DNA REPLICATION; DNA-BINDING PROTEINS; HUMANS; IMMUNOPRECIPITATION; MINICHROMOSOME MAINTENANCE PROTEINS; MULTIPROTEIN COMPLEXES; OLIGONUCLEOTIDES; SF9 CELLS; SPODOPTERA;

EID: 84889643278     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1320202110     Document Type: Article

References (28)

1. Coleman TR, Carpenter PB, Dunphy WG (1996) The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts. Cell 87(1): 53-63
2. Evrin C, et al. (2009) A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA replication. Proc Natl Acad Sci USA 106(48): 20240-20245
3. Remus D, et al. (2009) Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing. Cell 139(4):719-730
4. Labib K (2010) How do Cdc7 and cyclin-dependent kinases trigger the initiation of chromosome replication in eukaryotic cells? Genes Dev 24(12):1208-1219
5. Araki H (2011) Initiation of chromosomal DNA replication in eukaryotic cells; Contribution of yeast genetics to the elucidation. Genes Genet Syst 86(3):141-149
6. Thu YM, Bielinsky AK (2013) Enigmatic roles of Mcm10 in DNA replication. Trends Biochem Sci 38(4):184-194
7. Zhu W, et al. (2007) Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication. Genes Dev 21(18):2288-2299
8. Boos D, Yekezare M, Diffley JF (2013) Identification of a heteromeric complex that promotes DNA replication origin firing in human cells. Science 340(6135):981-984
9. Im JS, et al. (2009) Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. Proc Natl Acad Sci USA 106(37): 15628-15632
10. Gambus A, et al. (2006) GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks. Nat Cell Biol 8(4):358-366
11. Pacek M, Tutter AV, Kubota Y, Takisawa H, Walter JC (2006) Localization of MCM2-7, Cdc45, and GINS to the site of DNA unwinding during eukaryotic DNA replication. Mol Cell 21(4):581-587
12. Moyer SE, Lewis PW, Botchan MR (2006) Isolation of the Cdc45/Mcm2-7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase. Proc Natl Acad Sci USA 103(27):10236-10241
13. Ilves I, Petojevic T, Pesavento JJ, Botchan MR (2010) Activation of the MCM2-7 helicase by association with Cdc45 and GINS proteins. Mol Cell 37(2):247-258
14. Kang YH, Galal WC, Farina A, Tappin I, Hurwitz J (2012) Properties of the human Cdc45/Mcm2-7/GINS helicase complex and its action with DNA polymerase epsilon in rolling circle DNA synthesis. Proc Natl Acad Sci USA 109(16):6042-6047
15. Spencer F, Gerring SL, Connelly C, Hieter P (1990) Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae. Genetics 124(2):237-249
16. Miles J, Formosa T (1992) Evidence that POB1, a Saccharomyces cerevisiae protein that binds to DNA polymerase alpha, acts in DNA metabolism in vivo. Mol Cell Biol 12(12): 5724-5735
17. Williams DR, McIntosh JR (2002) mcl1+, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation. Eukaryot Cell 1(5):758-773
18. Tsutsui Y, et al. (2005) Genetic and physical interactions between Schizosaccharomyces pombe Mcl1 and Rad2, Dna2 and DNA polymerase alpha: Evidence for a multifunctional role of Mcl1 in DNA replication and repair. Curr Genet 48(1):34-43
19. Bermudez VP, Farina A, Tappin I, Hurwitz J (2010) Influence of the human cohesion establishment factor Ctf4/AND-1 on DNA replication. J Biol Chem 285(13):9493-9505
20. Miles J, Formosa T (1992) Protein affinity chromatography with purified yeast DNA polymerase alpha detects proteins that bind to DNA polymerase. Proc Natl Acad Sci USA 89(4):1276-1280
21. Tanaka H, et al. (2009) Ctf4 coordinates the progression of helicase and DNA polymerase alpha. Genes Cells 14(7):807-820
22. Gambus A, et al. (2009) A key role for Ctf4 in coupling the MCM2-7 helicase to DNA polymerase alpha within the eukaryotic replisome. EMBO J 28(19):2992-3004
23. Köhler A, Schmidt-Zachmann MS, Franke WW (1997) AND-1, a natural chimeric DNAbinding protein, combines an HMG-box with regulatory WD-repeats. J Cell Sci 110(Pt 9): 1051-1062
24. Gosnell JA, Christensen TW (2011) Drosophila Ctf4 is essential for efficient DNA replication and normal cell cycle progression. BMC Mol Biol 12:13
25. Harris SD, Hamer JE (1995) sepB: An Aspergillus nidulans gene involved in chromosome segregation and the initiation of cytokinesis. EMBO J 14(21):5244-5257
26. Lopez-Contreras AJ, et al. (2013) A proteomic characterization of factors enriched at nascent DNA molecules. Cell Rep 3(4):1105-1116
27. Kouprina N, et al. (1992) CTF4 (CHL15) mutants exhibit defective DNA metabolism in the yeast Saccharomyces cerevisiae. Mol Cell Biol 12(12):5736-5747
28. Sengupta S, van Deursen F, de Piccoli G, Labib K (2013) Dpb2 integrates the leadingstrand DNA polymerase into the eukaryotic replisome. Curr Biol 23(7):543-552