Genetic and physical interactions between Schizosaccharomyces pombe Mcl1 and Rad2, Dna2 and DNA polymerase a: Evidence for a multifunctional role of Mcl1 in DNA replication and repair

Current Genetics

Volumn 48, Issue 1, 2005, Pages 34-43

  Tsutsui, Yasuhiro ^a,b   Morishita, Takashi ^a   Natsume, Toyoaki ^b   Yamashita, Kentaro ^a   Iwasaki, Hiroshi ^c   Yamao, Fumiaki ^b   Shinagawa, Hideo ^a  

^a OSAKA UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF GENETICS   (Japan)

^c YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

DNA repair; Flap endonuclease 1; Mcl1; Okazaki fragment; Schizosaccharomyces pombe

Indexed keywords

DNA DIRECTED DNA POLYMERASE ALPHA; DOUBLE STRANDED RNA; EXONUCLEASE; FLAP ENDONUCLEASE; MUTANT PROTEIN; OKAZAKI FRAGMENT; PROTEIN MCL 1; PROTEIN RAD2; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; CHROMOSOME REPLICATION; CHROMOSOME SEGREGATION; CONTROLLED STUDY; DNA CONTENT; DNA DAMAGE; DNA REPAIR; DNA REPLICATION; DNA SYNTHESIS; FUNGAL CELL; FUNGAL STRAIN; FUNGUS GROWTH; GENE INTERACTION; GENE MUTATION; GENOTYPE; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN INTERACTION; PULSED FIELD GEL ELECTROPHORESIS; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; TEMPERATURE SENSITIVITY; TWO HYBRID SYSTEM;

CLONING, MOLECULAR; DNA; DNA DAMAGE; DNA POLYMERASE I; DNA REPAIR; DNA REPLICATION; ELECTROPHORESIS, GEL, PULSED-FIELD; ENDODEOXYRIBONUCLEASES; FLAP ENDONUCLEASES; MUTATION; NEOPLASM PROTEINS; PLASMIDS; PROTO-ONCOGENE PROTEINS C-BCL-2; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS; TWO-HYBRID SYSTEM TECHNIQUES;

SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

EID: 23144459109     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-005-0584-2     Document Type: Article

References (46)

1. Bae SH, Seo YS (2000) Characterization of the enzymatic properties of the yeast Dna2 Helicase/endonuclease suggests a new model for Okazaki fragment processing. J Biol Chem 275:38022-38031
2. Bailis JM, Bernard P, Antonelli R, Allshire RC, Forsburg SL (2003) Hsk1-Dfp1 is required for heterochromatin-mediated cohesion at centromeres. Nat Cell Biol 5:1111-1116 10.1038/ncb1069
3. Barbet N, Muriel WJ, Carr AM (1992) Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114:59-66 10.1016/0378-1119(92)90707-V
4. Basi G, Schmid E, Maundrell K (1993) TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility. Gene 123:131-136 10.1016/0378-1119(93)90552-E
5. Bhaumik D, Wang TS (1998) Mutational effect of fission yeast Pol a on cell cycle events. Mol Biol Cell 9:2107-2123
6. Brown GW, Kelly TJ (1999) Cell cycle regulation of Dfp1, an activator of the Hsk1 protein kinase. Proc Natl Acad Sci USA 96:8443-8448
7. Budd ME, Campbell JL (1997) A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function. Mol Cell Biol 17:2136-2142 1:CAS:528:DyaK2sXhvFOhsrs%3D
8. Cao Y, Kogoma T (1995) The mechanism of recA polA lethality: Suppression by RecA-independent recombination repair activated by the lexA (Def) mutation in Escherichia coli. Genetics 139:1483-1494 1:CAS:528:DyaK28Xht1Chtbc%3D
9. Craven RA, Griffiths DJ, Sheldrick KS, Randall RE, Hagan IM, Carr AM (1998) Vectors for the expression of tagged proteins in Schizosaccharomyces pombe. Gene 221:59-68 10.1016/S0378-1119(98)00434-X
10. D'Urso G, Grallert B, Nurse P (1995) DNA polymerase alpha, a component of the replication initiation complex, is essential for the checkpoint coupling S phase to mitosis in fission yeast. J Cell Sci 108:3109-3118
11. Formosa T, Nittis T (1999) Dna2 mutants reveal interactions with Dna polymerase alpha and Ctf4, a Pol alpha accessory factor, and show that full Dna2 helicase activity is not essential for growth. Genetics 151:1459-1470 1:CAS:528:DyaK1MXisV2rsrc%3D
12. Fousteri MI, Lehmann AR (2000) A novel SMC protein complex in Schizosaccharomyces pombe contains the Rad18 DNA repair protein. EMBO J 19:1691-1702 10.1093/emboj/19.7.1691
13. Haber JE (1998) The many interfaces of Mre11. Cell 95:583-586 10.1016/ S0092-8674(00)81626-8
14. Hanna JS, Kroll ES, Lundblad V, Spencer FA (2001) Saccharomyces cerevisiae CTF18 and CTF4 are required for sister chromatid cohesion. Mol Cell Biol 21:3144-3158 10.1128/MCB.21.9.3144-3158.2001
15. Harris SD, Hamer JE (1995) sepB: An Aspergillus nidulans gene involved in chromosome segregation and the initiation of cytokinesis. EMBO J 14:5244-5257 1:CAS:528:DyaK2MXps1Kmtr8%3D
16. + reveal that Dna2 plays an essential role in Okazaki fragment metabolism. Genetics 155:1055-1067 1:CAS:528:DC%2BD3cXlsFSktbs%3D
17. Kouprina N, Kroll E, Bannikov V, Bliskovsky V, Gizatullin R, Kirillov A, Shestopalov B, Zakharyev V, Hieter P, Spencer F, Larionov V (1992) CTF4 (CHL15) mutants exhibit defective DNA metabolism in the yeast Saccharomyces cerevisiae. Mol Cell Biol 12:5736-5747 1:STN:280:ByyD2sfnslE%3D
18. Kouprina N, Tsouladze A, Koryabin M, Hieter P, Spencer F, Larionov V (1993) Identification and genetic mapping of CHL genes controlling mitotic chromosome transmission in yeast. Yeast 9:11-19 1:CAS:528:DyaK3sXhs1ahurc%3D
19. +, a Schizosaccharomyces pombe gene related to Saccharomyces cerevisiae CDC7, is required for chromosomal replication. EMBO J 14:3094-3104 1:CAS:528:DyaK2MXntVektbY%3D
20. Mazin AV, Bornarth CJ, Solinger JA, Heyer WD, Kowalczykowski SC (2000) Rad54 protein is targeted to pairing loci by the Rad51 nucleoprotein filament. Mol Cell 6:583-592 10.1016/S1097-2765(00)00057-5
21. Miles J, Formosa T (1992) Evidence that POB1, a Saccharomyces cerevisiae protein that binds to DNA polymerase alpha, acts in DNA metabolism in vivo. Mol Cell Biol 12:5724-5735 1:CAS:528:DyaK3sXks1Ols78%3D
22. Moreno S, Klar A, Nurse P (1991) Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol 194:795-823 1:CAS:528:DyaK3MXit1ahs7w%3D
23. Morikawa H, Morishita T, Kawane S, Iwasaki H, Carr AM, Shinagawa H (2004) Rad62 protein functionally and physically associates with the smc5/smc6 protein complex and is required for chromosome integrity and recombination repair in fission yeast. Mol Cell Biol 24:9401-9413 10.1128/MCB.24.21.9401-9413.2004
24. Morishita T, Tsutsui Y, Iwasaki H, Shinagawa H (2002) The Schizosaccharomyces pombe rad60 gene is essential for repairing double-strand DNA breaks spontaneously occurring during replication and induced by DNA-damaging agents. Mol Cell Biol 22:3537-3548 10.1128/ MCB.22.10.3537-3548.2002
25. Murakami H, Okayama H (1995) A kinase from fission yeast responsible for blocking mitosis in S phase. Nature 374:817-819 10.1038/374817a0
26. +, a gene involved in the repair of radiation damage and replication fidelity. J Cell Sci 109:73-81
27. Murray JM, Tavassoli M, al-Harithy R, Sheldrick KS, Lehmann AR, Carr AM, Watts FZ (1994) Structural and functional conservation of the human homolog of the Schizosaccharomyces pombe rad2 gene, which is required for chromosome segregation and recovery from DNA damage. Mol Cell Biol 14:4878-4888 1:CAS:528:DyaK2cXlt1KmtLk%3D
28. van Nocker S, Ludwig P (2003) The WD-repeat protein superfamily in Arabidopsis: Conservation and divergence in structure and function. BMC Genomics 4:50 10.1186/1471-2164-4-50
29. Reagan MS, Pittenger C, Siede W, Friedberg EC (1995) Characterization of a mutant strain of Saccharomyces cerevisiae with a deletion of the RAD27 gene, a structural homolog of the RAD2 nucleotide excision repair gene. J Bacteriol 177:364-371 1:CAS:528:DyaK2MXjtFOqsL4%3D
30. Ryu GH, Tanaka H, Kim DH, Kim JH, Bae SH, Kwon YN, Rhee JS, MacNeill SA, Seo YS (2004) Genetic and biochemical analyses of Pfh1 DNA helicase function in fission yeast. Nucleic Acids Res 32:4205-4216 10.1093/nar/ gkh720
31. Sazer S, Sherwood SW (1990) Mitochondrial growth and DNA synthesis occur in the absence of nuclear DNA replication in fission yeast. J Cell Sci 97:509-516
32. Smith CL, Matsumoto T, Niwa O, Klco S, Fan JB, Yanagida M, Cantor CR (1987) An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis. Nucleic Acids Res 15:4481-4489 1:CAS:528:DyaL2sXkslajtbY%3D
33. Sung P (1994) Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein. Science 265:1241-1243 1:CAS:528:DyaK2cXmt1emsr8%3D
34. Sung P (1997) Yeast Rad55 and Rad57 proteins form a heterodimer that functions with replication protein A to promote DNA strand exchange by Rad51 recombinase. Genes Dev 11:1111-1121 1:CAS:528:DyaK2sXjtlartbk%3D
35. Sung P, Robberson DL (1995) DNA strand exchange mediated by a RAD51-ssDNA nucleoprotein filament with polarity opposite to that of RecA. Cell 82:453-461 10.1016/0092-8674(95)90434-4
36. Symington LS (1998) Homologous recombination is required for the viability of rad27 mutants. Nucleic Acids Res 26:5589-5595 10.1093/nar/ 26.24.5589
37. Tanaka K, Yonekawa T, Kawasaki Y, Kai M, Furuya K, Iwasaki M, Murakami H, Yanagida M, Okayama H (2000) Fission yeast Eso1p is required for establishing sister chromatid cohesion during S phase. Mol Cell Biol 20:3459-3469 10.1128/MCB.20.10.3459-3469.2000
38. + gene encodes an essential 5′ to 3′ DNA helicase required for the completion of S-phase. Nucleic Acids Res 30:4728-4739 10.1093/nar/gkf590
39. Tavassoli M, Shayeghi M, Nasim A, Watts FZ (1995) Cloning and characterisation of the Schizosaccharomyces pombe rad32 gene: A gene required for repair of double strand breaks and recombination. Nucleic Acids Res 23:383-388 1:CAS:528:DyaK2MXksVaksL0%3D
40. Tsay HJ, Choe YH, Neville CM, Schmidt J (1992) CTF4, a chicken transcription factor of the helix-loop-helix class A family. Nucleic Acids Res 20:2624 1:CAS:528:DyaK3sXkt1Wqu7w%3D
41. Tsutsui Y, Morishita T, Iwasaki H, Toh H, Shinagawa H (2000) A recombination repair gene of Schizosaccharomyces pombe, rhp57, is a functional homolog of the Saccharomyces cerevisiae RAD57 gene and is phylogenetically related to the human XRCC3 gene. Genetics 154:1451-1461 1:CAS:528:DC%2BD3cXjtVanu74%3D
42. Tsutsui Y, Khasanov FK, Shinagawa H, Iwasaki H, Bashkirov VI (2001) Multiple interactions among the components of the recombinational DNA repair system in Schizosaccharomyces pombe. Genetics 159:91-105 1:CAS:528:DC%2BD3MXns12js70%3D
43. + gene involved in DNA repair and telomere maintenance. Mol Cell Biol 23:6553-6563 10.1128/ MCB.23.18.6553-6563.2003
44. Vallen EA, Cross FR (1995) Mutations in RAD27 define a potential link between G1 cyclins and DNA replication. Mol Cell Biol 15:4291-4302 1:CAS:528:DyaK2MXntVGitLw%3D
45. Van Komen S, Petukhova G, Sigurdsson S, Stratton S, Sung P (2000) Superhelicity-driven homologous DNA pairing by yeast recombination factors Rad51 and Rad54. Mol Cell 6:563-572 10.1016/S1097-2765(00)00055-1
46. +, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation. Eukaryot Cell 1:758-773 10.1128/ EC.1.5.758-773.2002