Differential phenotypic behaviors of human degenerative nucleus pulposus cells under normoxic and hypoxic conditions: Influence of oxygen concentration during isolation, expansion, and cultivation

Spine Journal

Volumn 13, Issue 11, 2013, Pages 1590-1596

  Yang, Shu Hua ^a   Hu, Ming Hsiao ^a,b   Sun, Yuan Hui ^a   Lin, Feng Huei ^b  

^a NATIONAL TAIWAN UNIVERSITY HOSPITAL   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

Cell culture; Hypoxia; Nucleus pulposus

Indexed keywords

AGGRECAN; COLLAGEN TYPE 2; COLLAGENASE 3; GELATINASE A; HYPOXIA INDUCIBLE FACTOR 1ALPHA; HYPOXIA INDUCIBLE FACTOR 2ALPHA; RNA;

ARTICLE; CELL EXPANSION; CELL HYPOXIA; CELL ISOLATION; CELL PROLIFERATION; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; LUMBAR DISK; NUCLEUS PULPOSUS; OXYGEN CONCENTRATION; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION;

CELL CULTURE; HYPOXIA; NUCLEUS PULPOSUS;

ANOXIA; CELL PROLIFERATION; HUMANS; HYPOXIA-INDUCIBLE FACTOR 1, ALPHA SUBUNIT; INTERVERTEBRAL DISC; INTERVERTEBRAL DISC DEGENERATION; MATRIX METALLOPROTEINASES; OXYGEN;

EID: 84889084088     PISSN: 15299430     EISSN: 18781632     Source Type: Journal    
DOI: 10.1016/j.spinee.2013.05.025     Document Type: Article

References (30)

1. G. Waddell Low back pain: a twentieth century health care enigma Spine 21 1996 2820 2825
2. J.H. Adler, M. Schoenbaum, and R. Silberberg Early onset of disk degeneration and spondylosis in sand rats (Psammomys obesus) Vet Pathol 20 1983 13 22
3. X. Phélip Why the back of the child? Eur Spine J 8 1999 426 428
4. J. Antoniou, T. Steffen, and F. Nelson The human lumbar intervertebral disc. Evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration J Clin Invest 98 1996 996 1003
5. T.M. Ganey, and H.J. Meisel A potential role for cell-based therapeutics in the treatment of intervertebral disc herniation Eur Spine J 11 2002 S206 S214
6. H.J. Meisel, T. Ganey, and W.C. Hutton Clinical experience in cell-based therapeutics: intervention and outcome Eur Spine J 15 2006 S397 S405
7. H.J. Meisel, V. Siodla, and T. Ganey Clinical experience in cell-based therapeutics: disc chondrocyte transplantation - a treatment for degenerated or damaged intervertebral disc Biomol Eng 24 2007 5 21
8. J.Y. Wang, A.E. Baer, V.B. Kraus, and L.A. Sutton Intervertebral disc cells exhibit differences in gene expression in alginate and monolayer culture Spine 26 2001 1741 1751
9. J.P. Urban The role of the physiochemical environment in determining disc cell behaviour Biochem Soc Trans 30 2002 858 864
10. J.P. Urban, S. Smith, and J.C.T. Fairbank Nutrition of the intervertebral disc Spine 29 2004 2700 2709
11. J.W. Stairmand, S. Holm, and J.P. Urbna Factors influencing oxygen concentration gradients in the intervertebral disc. A theoretical analysis Spine 16 1991 444 449
12. F. Mwale, I. Ciobanu, and D. Giannitsios Effect of oxygen levels on proteoglycan synthesis by intervertebral discs Spine 36 2011 E131 E138
13. A. Agrawal, A. Guttapalli, and S. Narayan Normoxic stabilization of HIF-1α drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk Am J Physiol Cell Physiol 293 2007 C621 C631
14. A. Bertolo, L. Ettinger, and N. Aebli The in vitro effects of dexamethasone, insulin and triiodothysonine on degenerative human intervertebral disc cells under normoxic and hypoxic conditions Eur Cells Mater 21 2011 221 229
15. W.M. Erwin, F. Las Heras, and D. Islam The regenerative capacity of the notochordal cell: tissue constructs generated in vitro under hypoxic conditions J Neurosurg Spine 10 2009 513 521
16. S.H. Yang, C.C. Lin, and M.H. Hu Influence of age-related degeneration on regenerative potential of human nucleus pulposus cells J Orthop Res 28 2010 379 383
17. H.S. An, E.J. Thonar, and K. Masuda Biological repair of intervertebral disc Spine 28 2003 S86 S92
18. J.I. Sive, P. Baird, and M. Jeziorsk Expression of chondrocyte markers by cells of normal and degenerative intervertebral discs Mol Pathol 55 2002 91 97
19. R.H. Pearce, B.J. Grimmer, and M.E. Adams Degeneration and the chemical composition of the human intervertebral disc J Orthop Res 5 1987 198 205
20. W.C. Bae, and K. Masuda Emerging technologies for molecular therapy for intervertebral disc degeneration Clin Orthop N Am 42 2011 585 601
21. K. Masuda, and H.S. An Prevention of disc degeneration with growth factors Eur Spine J 15 2006 S422 S432
22. H. Murakami, S.T. Yoon, and E.S. Attallah-Wasif The expression of anabolic cytokines in intervertebral discs in age-related degeneration Spine 31 2006 1770 1774
23. J.E. Lafont, S. Talma, C. Hopfgarten, and C.L. Murphy Hypoxia promotes the differentiated human articular chondrocyte phenotype through SOX9-dependent and -independent pathways J Biol Chem 283 2008 4778 4786
24. M. Husa, R. Liu-Bryan, and R. Terkeltaub Shifting HIFs in osteoarthritis Nat Med 16 2010 641 644
25. A. Agrawal, S. Gajghate, and H. Smith Cited 2 modulates hypoxia-inducible factor-dependent expression of vascular endothelial growth factor in nucleus pulposus cells of the rat intervertebral disc Arthritis Rheum 58 2008 3798 3808
26. E. Duval, S. Leclercq, and J.M. Elissalde Hypoxia-inducible factor 1α inhibits the fibroblast-like markers type I and type III collagen during hypoxia-induced chondrocyte redifferentiation: hypoxia not only induces type II collagen and aggrecan, but it also inhibits type I and type III collagen in the hypoxia-inducible factor 1α-dependent redifferentiation of chondrocytes Arthritis Rheum 60 2009 3038 3048
27. T. Saito, A. Fukai, and A. Mabuchi Transcriptional regulation of endochondral ossification by HIF-2α during skeletal growth and osteoarthritis development Nat Med 16 2010 678 686
28. S. Yang, J. Kim, and J.H. Ryu Hypoxia-inducible factor-2α is a catabolic regulator of osteoarthritic cartilage destruction Nat Med 16 2010 687 693
29. 2 tension Proc Natl Acad Sci USA 92 1995 5510 5514
30. S. Salceda, and J. Caro Hypoxia-inducible factor 1α (HIF-1α) protein is rapidly degraded by the ubiquitin-preteasome system under normoxic conditions J Biol Chem 272 1997 22642 22647