Immunolocalization of a beta-defensin (Tu-BD-1) in the skin and subdermal granulocytes of turtles indicate the presence of an antimicrobial skin barrier

Annals of Anatomy

Volumn 195, Issue 6, 2013, Pages 554-561

  Lorenzo, Alibardi ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Beta defensins; Granulocytes; Immunocytochemistry; Skin; Turtles

Indexed keywords

BETA DEFENSIN 1; PROTEIN TU BD 1; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELLULAR DISTRIBUTION; CONNECTIVE TISSUE; CORNEOCYTE; EPIDERMIS; GRANULOCYTE; IMMUNOLOCALIZATION; NONHUMAN; PROTEIN LOCALIZATION; SECRETORY GRANULE; SKIN; SKIN BLOOD VESSEL; SKIN CELL; TURTLE;

TESTUDINES;

BETA-DEFENSINS; GRANULOCYTES; IMMUNOCYTOCHEMISTRY; SKIN; TURTLES;

AMINO ACID SEQUENCE; ANIMALS; BETA-DEFENSINS; DERMIS; EPIDERMIS; EPITOPES; GRANULOCYTES; IMMUNITY; IMMUNOHISTOCHEMISTRY; MICROSCOPY, IMMUNOELECTRON; MICROTOMY; MOLECULAR SEQUENCE DATA; SKIN; SKIN PHYSIOLOGICAL PHENOMENA; SPECIES SPECIFICITY; TURTLES;

EID: 84888428221     PISSN: 09409602     EISSN: 16180402     Source Type: Journal    
DOI: 10.1016/j.aanat.2013.07.001     Document Type: Article

References (29)

1. Alibardi L. Immunocytochemical observations on the cornification of soft and hard epidermis in the turtle Chrysemis picta. Zoology 2002, 105:31-44.
2. Alibardi L. Ultrastructural and immunocytochemical observations on the process of horny growth in chelonian shell. Acta Histochem. 2006, 108:149-162.
3. Alibardi L. Granulocytes of reptiles contain beta-defensin-like peptides: a comparative ultrastructural survey. J. Morphol. 2013, 274(8):877-886.
4. Alibardi L., Celeghin A., Dalla Valle L. Wounding in lizards results in the release of beta-defensins at the wound site and formation of an antimicrobial barrier. Dev. Comp. Immunol. 2012, 36:557-565.
5. Antcheva N., Zelezetsky I., Tossi A. Cationic antimicrobial peptides - the defensins. The Handbook of Biologically Active Peptides 2006, 55-66. Elsevier, Burlington, MA. A.J. Kastin (Ed.).
6. Benato F., Dalla Valle L., Skobo T., Alibardi L. Biomolecular identification of beta-defensin-like peptides from the skin of the soft-shelled turtle Apalone spinifera. J. Exp. Zool. B: Mol. Dev. Evol. 2013, 320(4):210-217.
7. Chattopadhyay S., Sinha N.K., Banerjee S., Roy D., Chattopadhyay D., Roy S. Small cationic protein from marine turtle has β-defensin-like fold and antibacterial and antiviral activity. Proteins 2006, 64:524-531.
8. Chung W.O., Dale B.A. Innate immune response of oral and foreskin keratinocytes: utilization of different signaling pathways by various bacterial species. Infect. Immun. 2004, 72:352-358.
9. Dalla Valle L., Benato F., Maistro S., Quinzani S., Alibardi L. Bioinformatic and molecular characterization of beta-defensin like peptides isolated from the green lizard Anolis carolinensis. Dev. Comp. Immunol. 2011, 36:222-229.
10. Dalla Valle L., Benato F., Paccanaro M.C., Alibardi L. Bioinformatic and molecular characterization of cathelicidin-like peptides isolated from the green lizard Anolis carolinensis. Ital. J. Zool. 2013, 80(2):177-186.
11. Danilova N. The evolution of immune mechanisms. J. Exp. Zool. B 2006, 306:496-520.
12. Garreis F., Schlorf T., Worlitzsch D., Steven P., Brauer L., Jager K., Paulsen F.P. Role of human beta-defensins in innate immune defence at the ocular surface: arming and alarming corneal and conjunctival epithelial cells. Histochem. Cell Biol. 2010, 134:59-73.
13. Hellgren O., Ekblom R. Evolution of a cluster of innate immune genes (β-defensins) along the ancestral lines of chicken and zebra finch. Immunome Res. 2010, 6:3-15.
14. Lakshminarayanan R., Vivekanandan S., Samy R.P., Banerjee Y., Chin-Jin E.O., Teo K.W., Jois S.D., Kini R.M., Valiyaveettil S. Structure, self-assembly, and dual role of a beta-defensin-like peptide from the Chinese soft-shelled turtle eggshell matrix. J. Am. Chem. Soc. 2008, 130:4660-4668.
15. Lynn D.J., Higgs R., Lloyd A.T., O'Farrelly C., Hervé-Grépinet V., Nys Y., Brinkman F.S., Yu P.L., Soulier A., Kaiser P., Zhang G., Lehrer R.I. Avian beta-defensin nomenclature: a community proposed update. Immunol. Lett. 2007, 110:86-89.
16. Matoltsy G.A., Huszar T. Keratinization of the reptilian epidermis: an ultrastructural study on the turtle skin. J. Ultrastruct. Res. 1972, 38:87-101.
17. Menzies B.E., Kenoyer A. Staphylococcus aureus infection of epidermal keratinocytes promotes expression of innate antimicrobial peptides. Infect. Immun. 2005, 73:5241-5244.
18. Nicastro G., Franzoni L., de Chiara C., Mancin A.C., Giglio J.R., Spisni A. Solution structure of crotamine, a Na+ channel affecting toxin from Crotalus durissuss terrificus venom. Eur. J. Biochem. 2003, 270:1969-1979.
19. Oren A., Ganz T., Liu L., Meerloo T. In human epidermis, beta-defensin 2 is packaged in lamellar bodies. Exp. Mol. Pathol. 2003, 74:180-182.
20. Pata S., Yaraksa N., Daduang S., Temsiripong Y., Svasti J., Araki T., Thammasirirak S. Characterization of the novel antibacterial peptide Leucrocin from crocodile (Crocodylus siamensis) white blood cell extracts. Dev. Comp. Immunol. 2011, 35:545-553.
21. Pazgier M., Hoover D.M., Yang D., Lu W., Lubkowski J. Human beta-defensins. Cell Mol. Life Sci. 2006, 63:1294-1313.
22. Rice W.G., Ganz T., Kinkade J.M., Selsted M.E., Parmley R.T. Defensin-rich dense granules of human neutrophils. Blood 1987, 70:757-765.
23. Sahl H.G., Pag U., Bonness S., Wagner S., Antcheva N., Tossi A. Mammalian defensins: structures and mechanism of antibiotic activity. J. Leukoc. Biol. 2005, 77:466-475.
24. Stegemann C., Kolobov A., Leonova Y.F., Knappe D., Shamova O., Ovchinnikova T.V., Kokryakov V.N., Hoffmann R. Isolation, purification and de novo sequencing of TBD-1, the first beta-defensin from leucocytes of reptiles. Proteomics 2009, 9:1364-1373.
25. Sugiarto H., Yu P.L. Avian antimicrobial peptides: the defense role of beta-defensins. Biochem. Biophys. Res. Commun. 2004, 323:721-727.
26. Taylor K., Barran P.E., Dorin J.R. Structure-activity relationships in B-defensin peptides. Pept. Sci. 2008, 90:1-7.
27. Wang Y., Hong J., Liu X., Yang H., Liu R., Wu J., Wang A., Lin D., Lai R. Snake cathelicidin from Bungarus fasciatus is a potent peptide antibiotics. PLoS ONE 2008, 3:e3217.
28. Zhao H., Gan T.X., Liu X.D., Jin Y., Lee W.H., Shen J.H., Zhang Y. Identification and characterization of novel reptile cathelicidins from elapid snakes. Peptides 2008, 29:1685-1691.
29. Zimmerman L.M., Voegel L.A., Bowden R.M. Commentary. Understanding the vertebrate immune system: insights from the reptilian perspective. J. Exp. Biol. 2010, 213:661-671.