Trafficking along the secretory pathway in drosophila cell line and tissues: A light and electron microscopy approach

Methods in Cell Biology

Volumn 118, Issue , 2013, Pages 35-49

  Zacharogianni, Margarita ^a   Rabouille, Catherine ^a,b  

^a HUBRECHT INSTITUTE   (Netherlands)

^b UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

Anterograde protein transport assay; Copper sulfate inducible expression; Drosophila; ER exit sites; Golgi; Immunofluorescence; Light and electron microscopy; RNAi; S2 cells; Sec16; Secretory pathway; Transfection

Indexed keywords

BUFFER; GREEN FLUORESCENT PROTEIN;

ANIMAL; ANTEROGRADE PROTEIN TRANSPORT ASSAY; ARTICLE; BIOSYNTHESIS; CELL LINE; COPPER SULFATE-INDUCIBLE EXPRESSION; CYTOLOGY; DROSOPHILA; DROSOPHILA MELANOGASTER; ER EXIT SITES; FEMALE; FLUORESCENCE MICROSCOPY; GENE SILENCING; GENETIC TRANSFECTION; GENETICS; GOLGI; IMMUNOELECTRON MICROSCOPY; IMMUNOFLUORESCENCE; LIGHT AND ELECTRON MICROSCOPY; METABOLISM; OVARY; PROTEIN TRANSPORT; RNA INTERFERENCE; RNAI; S2 CELLS; SALIVARY GLAND; SEC16; SECRETORY PATHWAY;

ANTEROGRADE PROTEIN TRANSPORT ASSAY; COPPER SULFATE-INDUCIBLE EXPRESSION; DROSOPHILA; ER EXIT SITES; GOLGI; IMMUNOFLUORESCENCE; LIGHT AND ELECTRON MICROSCOPY; RNAI; S2 CELLS; SEC16; SECRETORY PATHWAY; TRANSFECTION;

ANIMALS; BUFFERS; CELL LINE; DROSOPHILA MELANOGASTER; FEMALE; GENE KNOCKDOWN TECHNIQUES; GREEN FLUORESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MICROSCOPY, IMMUNOELECTRON; OVARY; PROTEIN TRANSPORT; RNA INTERFERENCE; SALIVARY GLANDS; SECRETORY PATHWAY; TRANSFECTION;

EID: 84888214408     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-417164-0.00003-3     Document Type: Chapter

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