Monitoring microbiological changes in drinking water systems using a fast and reproducible flow cytometric method

Water Research

Volumn 47, Issue 19, 2013, Pages 7131-7142

  Prest, E I ^a   Hammes, F ^b   Kotzsch S ^b   van Loosdrecht, M C M ^a   Vrouwenvelder, J S ^a,c,d  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

^b SWISS FEDERAL INSTITUTE OF AQUATIC SCIENCE AND TECHNOLOGY   (Switzerland)

^c EUROPEAN CENTRE OF EXCELLENCE FOR SUSTAINABLE WATER TECHNOLOGY   (Netherlands)

^d KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Saudi Arabia)

Author keywords

HNA; A.u; AOC; Biological stability; Distribution network; Drinking water; FCM; Flow cytometry (FCM); HNA; Household; LNA; Low and high nucleic acid (LNA and HNA) content bacteria; RSD; TCC

Indexed keywords

AQUATIC ORGANISMS; BACTERIA; ELECTRIC POWER DISTRIBUTION; FLOW CYTOMETRY; FLUORESCENCE; NUCLEIC ACIDS; QUALITY CONTROL; WATER QUALITY; WATER TREATMENT;

%HNA; BACTERIOLOGICAL QUALITY; BIOLOGICAL STABILITY; DETECTION AND QUANTIFICATIONS; HOUSEHOLD; MICROBIAL WATER QUALITY; RELATIVE STANDARD DEVIATIONS; TRIPLICATE MEASUREMENT;

POTABLE WATER;

DRINKING WATER; NUCLEIC ACID;

AQUATIC ENVIRONMENT; COMMUNITY DYNAMICS; DETECTION METHOD; DRINKING WATER; FLOW CYTOMETRY; FLUORESCENCE; MICROBIOLOGY; NUCLEIC ACID; WATER QUALITY;

ACCURACY; AQUATIC ENVIRONMENT; ARTICLE; BACTERIAL GROWTH; CONCENTRATION (PARAMETERS); FLOW CYTOMETRY; FLUORESCENCE; MEASUREMENT; METHODOLOGY; MICROBIAL COMMUNITY; MICROBIOLOGICAL EXAMINATION; PRIORITY JOURNAL; WASTE WATER; WATER ANALYSIS; WATER CONTAMINATION; WATER SUPPLY;

BACTERIA (MICROORGANISMS);

%HNA; A.U; AOC; ARBITRARY UNITS; ASSIMILABLE ORGANIC CARBON; BIOLOGICAL STABILITY; DISTRIBUTION NETWORK; DRINKING WATER; FCM; FLOW CYTOMETER / FLOW CYTOMETRY; FLOW CYTOMETRY (FCM); HIGH NUCLEIC ACID; HNA; HOUSEHOLD; LNA; LOW AND HIGH NUCLEIC ACID (LNA AND HNA) CONTENT BACTERIA; LOW NUCLEIC ACID; PERCENTAGE OF HIGH NUCLEIC ACID CONTENT CELLS; RELATIVE STANDARD DEVIATION; RSD; TCC; TOTAL CELL COUNT;

BACTERIA; DRINKING WATER; FLOW CYTOMETRY; FLUORESCENCE; REPRODUCIBILITY OF RESULTS; WASTE WATER; WATER MICROBIOLOGY; WATER PURIFICATION;

EID: 84888132837     PISSN: 00431354     EISSN: 18792448     Source Type: Journal    
DOI: 10.1016/j.watres.2013.07.051     Document Type: Article

References (44)

1. Berney M., Vital M., Hulshoff I., Weilenmann H.U., Egli T., Hammes F. Rapid, cultivation-independent assessment of microbial viability in drinking water. Water Res. 2008, 42(14):4010-4018.
2. Boddy L., Wilkins M.F., Morris C.W. Pattern recognition in flow cytometry. Cytometry 2001, 44:195-209.
3. Bouvier T., Del Giorgio P.A., Gasol J.M. A comparative study of the cytometric characteristics of high and low nucleic-acid bacterioplankton cells from different aquatic ecosystems. Environ. Microbiol. 2007, 9(8):2050-2066.
4. De Roy K., Clement L., Thas O., Wang Y., Boon N. Flow cytometry for fast microbial community fingerprinting. Water Res. 2012, 46(3):907-919.
5. Gasol J.M., Del Giorgio P.A. Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities. Sci. Mar. 2000, 64(2):197-224.
6. Gasol J.M., Zweifel U.L., Peters F., Fuhrman J.A., Hagstrom A. Significance of size and nucleic acid content heterogeneity as measured by flow cytometry in natural planktonic bacteria. Appl. Environ. Microbiol. 1999, 65(10):4475-4483.
7. Günther S., Hubschmann T., Rudolf M., Eschenhagen M., Roske I., Harms H., Muller S. Fixation procedures for flow cytometric analysis of environmental bacteria. J. Microbiol. Meth. 2008, 75(1):127-134.
8. Hammes F., Broger T., Weilenmann H.U., Vital M., Helbing J., Bosshart U., Huber P., Peter Odermatt R., Sonnleitner B. Development and laboratory-scale testing of a fully automated online flow cytometer for drinking water analysis. Cytometry A 2012, 81(6):508-516.
9. Hammes F., Berney M., Egli T. Cultivation-independent assessment of bacterial viability. Adv. Biochem. Eng. Biotechnol. 2011, 124:123-150.
10. Hammes F., Egli T. Cytometric methods for measuring bacteria in water: advantages, pitfalls and applications. Anal. Bioanal. Chem. 2010, 397(3):1083-1095.
11. Hammes F., Vital M., Egli T. Critical evaluation of the volumetric "bottle effect" on microbial batch growth. Appl. Environ. Microbiol. 2010, 76(4):1278-1281.
12. Hammes F., Berney M., Wang Y., Vital M., Koster O., Egli T. Flow-cytometric total bacterial cell counts as a descriptive microbiological parameter for drinking water treatment processes. Water Res. 2008, 42(1-2):269-277.
13. Hammes F., Egli T. New method for assimilable organic carbon determination using flow-cytometric enumeration and a natural microbial consortium as inoculum. Environ. Sci. Technol. 2005, 39:3289-3294.
14. Ho L., Braun K., Fabris R., Hoefel D., Morran J., Monis P., Drikas M. Comparison of drinking water treatment process streams for optimal bacteriological water quality. Water Res. 2012, 46(12):3934-3942.
15. Hoefel D., Monis P.T., Grooby W.L., Andrews S., Saint C.P. Profiling bacterial survival through a water treatment process and subsequent distribution system. J. Appl. Microbiol. 2005, 99(1):175-186.
16. Hoefel D., Grooby W.L., Monis P.T., Andrews S., Saint C.P. Enumeration of water-borne bacteria using viability assays and flow cytometry: a comparison to culture-based techniques. J. Microbiol. Meth. 2003, 55(3):585-597.
17. Koch C., Fetzer I., Harms H., Muller S. CHIC22An automated approach for the detection of dynamic variations in complex microbial communities. Cytometry A 2013, 83(6):561-567.
18. Koch C., Fetzer I., Schmidt T., Harms H., Muller S. Monitoring functions in managed microbial systems by cytometric bar coding. Environ. Sci. Technol. 2013, 47(3):1753-1760.
19. Kötzsch S., Egli T. Kunststoffe in Kontakt mit Trinkwasser. Aqua Gas 2013, 44(3):44-52.
20. Lautenschlager K., Hwang C., Liu W.-T., Boon N., Köster O., Vrouwenvelder H., Egli T., Hammes F. A microbiology-based multi-parametric approach towards assessing biological stability in drinking water distribution networks. Water Res. 2013, 47(9):3015-3025.
21. Lautenschlager K., Boon N., Wang Y., Egli T., Hammes F. Overnight stagnation of drinking water in household taps induces microbial growth and changes in community composition. Water Res. 2010, 44(17):4868-4877.
22. Lebaron P., Servais P., Baudoux A.C., Bourrain M., Courties C., Parthuisot N. Variations of bacterial-specific activity with cell size and nucleic acid content assessed by flow cytometry. Aquat. Microb. Ecol. 2002, 28:131-140.
23. Lebaron P., Servais P., Agogue H., Courties C., Joux F. Does the high nucleic acid content of individual bacterial cells allow us to discriminate between active cells and inactive cells in aquatic systems?. Appl. Environ. Microbiol. 2001, 67(4):1775-1782.
24. Lebaron P., Parthuisot N., Catala P. Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems. Appl. Environ. Microbiol. 1998, 65(5):1725-1730.
25. Li W.K.W., Jellett J.F., Dickie P.M. DNA distributions in planktonic bacteria stained with TOTO or TO-PRO. Limnol. Oceanogr 1995, 40(8):1485-1495.
26. Longnecker K., Sherr B.F., Sherr E.B. Activity and phylogenetic diversity of bacterial cells with high and low nucleic acid content and electron transport system activity in an upwelling ecosystem. Appl. Environ. Microbiol. 2005, 71(12):7737-7749.
27. Muela A., Orruño M., Alonso M.L., Pazos M., Arana I., Alonso R.M., Jiménez R.M., Garaizabal I., Maguregui M.I., Barcina I. Microbiological parameters as an additional tool to improve wastewater treatment plant monitoring. Ecol. Indic. 2011, 11(2):431-437.
28. Muller S., Ullrich S., Losche A., Loffhagen N., Babel W. Flow cytometric techniques to characterise physiological states of Acinetobacter calcoaceticus. J. Microbiol. Meth. 2000, 40:67-77.
29. Phe M.H., Dossot M., Guilloteau H., Block J.C. Nucleic acid fluorochromes and flow cytometry prove useful in assessing the effect of chlorination on drinking water bacteria. Water Res. 2005, 39(15):3618-3628.
30. Pinto A.J., Xi C., Raskin L. Bacterial community structure in the drinking water microbiome is governed by filtration processes. Environ. Sci. Technol. 2012, 46(16):8851-8859.
31. Pomati F., Jokela J., Simona M., Veronesi M., Ibelings B.W. An automated platform for phytoplankton ecology and aquatic ecosystem monitoring. Environ. Sci. Technol. 2011, 45(22):9658-9665.
32. Ramseier M.K., von Gunten U., Freihofer P., Hammes F. Kinetics of membrane damage to high (HNA) and low (LNA) nucleic acid bacterial clusters in drinking water by ozone, chlorine, chlorine dioxide, monochloramine, ferrate(VI), and permanganate. Water Res. 2011, 45(3):1490-1500.
33. Robertson B.R., Button D.K. Characterizing aquatic bacteria according to population cell size, and apparentDNA content by flow cytometry. Cytometry 1989, 10:70-76.
34. Servais P., Casamayor E.O., Courties C., Catala P., Parthuisot N., Lebaron P. Activity and diversity of bacterial cells with high and low nucleic acid content. Aquat. Microb. Ecol. 2003, 33:41-51.
35. SLMB Determining the Total Cell Count and Ratios of High and Low Nucleic Acid Content Cells in Freshwater Using Flow Cytometry. Analysis Method 333.1, the Swiss Food Book 2012, (Schweizerische Lebensmittelbuch). Federal Office of Public Health, Switzerland.
36. Veal D.A., Deere D., Ferrari B., Piper J., Attfield P.V. Fluorescence staining and flow cytometry for monitoring microbial cells. J. Immunolog. Meth. 2000, 243:191-210.
37. Vila-Costa M., Gasol J.M., Sharma S., Moran M.A. Community analysis of high- and low-nucleic acid-containing bacteria in NW Mediterranean coastal waters using 16S rDNA pyrosequencing. Environ. Microbiol. 2012, 14(6):1390-1402.
38. Vital M., Dignum M., Magic-Knezev A., Ross P., Rietveld L., Hammes F. Flow cytometry and adenosine tri-phosphate analysis: alternative possibilities to evaluate major bacteriological changes in drinking water treatment and distribution systems. Water Res. 2012, 46(15):4665-4676.
39. Vital M., Stucki D., Egli T., Hammes F. Evaluating the growth potential of pathogenic bacteria in water. Appl. Environ. Microbiol. 2010, 76(19):6477-6484.
40. Vives-Rego J., Lebaron P., Nebe-von Caron G. Current and future applications of flow cytometry in aquatic microbiology. FEMS Microbiol. Rev. 2000, 24:429-448.
41. Wang Y., Hammes F., Boon N., Chami M., Egli T. Isolation and characterization of low nucleic acid (LNA)-content bacteria. ISME J. 2009, 3(8):889-902.
42. Wang Y., Hammes F., De Roy K., Verstraete W., Boon N. Past, present and future applications of flow cytometry in aquatic microbiology. Trends Biotechnol. 2010, 28(8):416-424.
43. Wang Y., Hammes F., Egli T. The impact of industrial-scale cartridge filtration on the native microbial communities from groundwater. Water Res. 2008, 42(16):4319-4326.
44. Zipper H., Brunner H., Bernhagen J., Vitzthum F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res. 2004, 32(12):e103.