Nucleic acid fluorochromes and flow cytometry prove useful in assessing the effect of chlorination on drinking water bacteria

Water Research

Volumn 39, Issue 15, 2005, Pages 3618-3628

  Phe, Meng Huot ^a   Dossot, Manuel ^a   Guilloteau, Hélène ^a   Block, Jean Claude ^a  

^a Laboratoire de Chimie Physique et Microbiologie pour l'Environnement   (France)

Author keywords

Bacteria culturability; Chlorination; Drinking water; Flow cytometry; Propidium iodide; SYBR Green II

Indexed keywords

BACTERIA; CHLORINE; DISINFECTION; NUCLEIC ACIDS; POTABLE WATER;

BACTERIA CULTRABILITY; FLOW CYTOMETRY (FCM); PROPIDIUM IODIDE; SYBR GREEN II;

CHLORINATION;

BACTERIAL DNA; CHLORINE DERIVATIVE; DRINKING WATER; FLUOROCHROME; NUCLEIC ACID; PROPIDIUM IODIDE;

BACTERIUM; CHLORINATION; DRINKING WATER;

ARTICLE; BACTERIAL MEMBRANE; BACTERIUM CONTAMINATION; BACTERIUM CULTURE; CHLORINATION; CONTROLLED STUDY; DISINFECTION; DNA DAMAGE; FLOW CYTOMETRY; MEMBRANE PERMEABILITY; NONHUMAN; PLATE COUNT; PRIORITY JOURNAL; REACTION TIME; RNA GEL BLOT ANALYSIS; STANDARDIZATION; WATER TREATMENT;

EID: 24344475250     PISSN: 00431354     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.watres.2005.06.002     Document Type: Article

References (29)

1. M. Albat, B. Baleux, B. Picot, J.M. Philipot, and J. Bontoux Developments of total Kjeldahl nitrogen and bacterial biomass along a potabilization line Water Res. 20 1986 237 245
2. J.M. Albrich, and J.K. Hurst Oxidative inactivation of Escherichia coli by hypochlorous acid FEBS Lett. 144 1982 157 161
3. M.R. Barer, and C.R. Harwood Bacterial viability and culturability Adv. Microb. Physiol. 41 1999 93 137
4. W.C. Barrette Jr., D.M. Hannum, W.D. Wheeler, and J.K. Hurst General mechanism for the bacterial toxicity of hypochlorous acid: abolition of ATP production Biochemistry 28 1989 9172 9178
5. M. Batté, B.R.M. Appenzeller, D. Grandjean, S. Fass, V. Gauthier, F. Jorand, L. Mathieu, M. Boualam, S. Saby, and J.C. Block Biofilms in drinking water distribution systems Rev. Environ. Sci. Biotechnol. 2 2003 147 168
6. C. Bernofsky Nucleotide chloramines and neutrophil-mediated cytotoxicity FASEB J. 5 1991 295 300
7. L. Boulos, M. Prévost, B. Barbeau, J. Coallier, and R. Desjardins LIVE/DEAD BacLight: application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water J. Microbiol. Methods 37 1999 77 86
8. C.J. Bunthof, S. van den Braak, P. Breeuwer, F.M. Rombouts, and T. Abee Rapid fluorescent assessment of the viability of stressed Lactococcus lactis Appl. Environ. Microbiol. 65 1999 3681 3689
9. R.R. Colwell D.J. Grimes Non-culturable Microorganisms in the Environment 2000 ASM Press Washington DC 354pp
10. H.M. Davey, and D.B. Kell Flow-cytometry and cell sorting of heterogeneous microbial populations - the importance of single-cell analyses Microbiol. Rev. 60 1996 641 649
11. J.M. Gasol, U.L. Zweifel, F. Peters, J.A. Furham, and A. Hagström Significance of size and nucleic acid content heterogeneity as measure by flow cytometry in natural planktonic bacteria Appl. Environ. Microbiol. 65 1999 4475 4483
12. G. Grégori, S. Citterio, A. Ghiani, M. Labra, S. Sgorbati, S. Brown, and M. Denis Resolution of viable and membrane-compromised bacteria in freshwater and marine waters based on analytical flow cytometry and nucleic acid double staining Appl. Environ. Microbiol. 67 2001 4662 4670
13. R.P. Haugland Handbook of fluorescent probes and research products ninth ed 2002 Molecular Probes Inc. Eugene 679pp
14. G. Herrera, A. Martinez, M. Blanco, and J.E. O'Connor Assessment of Escherichia coli B with enhanced permeability to fluorochromes for flow cytometric assays of bacterial cell function Cytometry 49 2002 62 69
15. D. Hoefel, W.L. Grooby, P.T. Monis, S. Andrews, and C.P. Saint Enumeration of water-borne bacteria using viability assays and flow cytometry: a comparison to culture-based techniques J. Microbiol. Methods 55 2003 585 597
16. P. Lebaron, N. Parthuisot, and P. Catala Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems Appl. Environ. Microbiol. 64 1998 1725 1730
17. P. Lebaron, P. Servais, H. Agogué, C. Courties, and F. Joux Does the high nucleic acid content of individual bacterial cells allow us to discriminate between active cells and inactive cells in aquatic systems? Appl. Environ. Microbiol. 67 2001 1775 1782
18. J. Lehtinen, J. Nuutila, and E.M. Lilius Green fluorescent protein-propidium iodide (GFP-PI) based assay for flow cytometric measurement of bacterial viability Cytometry 60A 2004 165 172
19. S.M. McKenna, and K.J.A. Davies Bacterial killing by phagocytes: potential role(s) of hypochlorous acid and hydrogen peroxide in protein turnover, DNA synthesis, and RNA synthesis Basic Life Sci. 49 1988 829 832
20. M.H. Phe, M. Dossot, and J.C. Block Chlorination effect on the fluorescence of nucleic acid staining dyes Water Res. 38 2004 3729 3737
21. W.A. Prütz Interactions of hypochlorous acid with pyrimidine nucleotides, and secondary reactions of chlorinated pyrimidines with GSH, NADH, and other substrates Arch. Biochem. Biophys. 349 1998 183 191
22. ème éd 1996 Dunod Paris 1384pp
23. S. Saby, I. Sibille, L. Mathieu, J.L. Paquin, and J.C. Block Influence of water chlorination on the counting of bacteria with DAPI (4′,6-diamidino- 2-phenylindole) Appl. Environ. Microbiol. 63 1997 1564 1569
24. P. Servais, G. Billen, C. Ventresque, and G.P. Bablon Microbial activity in GAC filters at the Choisy-le-Roi treatment plant J. Am. Water Works Assoc. 83 1991 62 68
25. H.J. Sips, and M.N. Hamers Mechanism of the bacterial action of myeloperoxidase: increased permeability of the Escherichia coli cell envelope Infect. Immun. 31 1981 11 16
26. E.L. Thomas Myeloperoxidase, hydrogen peroxide, chloride antimicrobial system: nitrogen-chlorine derivatives of bacterial components in bacterial action against Escherichia coli Infect. Immun. 23 1979 522 531
27. C. Venkobachar, L. Iyengar, and A.V.S. Prabhakara Rao Mechanism of disinfection: effect of chlorine on cell membrane functions Water Res. 11 1977 727 729
28. I. Williams, F. Paul, R. Jepras, I. Critcheley, M. Newman, J. Warrack, T. Giokirini, A.J. Hayes, P.F. Randerson, and W.A. Venables Flow cytometry and other techniques show that Staphylococcus aureus undergoes significant physiological changes in the early stages of surface-attached culture Microbiology 145 1999 1325 1333
29. U.L. Zweifel, and A. Hagström Total counts of marine bacteria include a large fraction of non-nucleoid-containing bacteria (Ghosts) Appl. Environ. Microbiol. 61 1995 2180 2185