Real time RT-PCR analysis of inflammatory mediator expression in recurrent airway obstruction-affected horses

Veterinary Immunology and Immunopathology

Volumn 156, Issue 3-4, 2013, Pages 190-199

  Padoan, E ^a,b   Ferraresso, S ^b   Pegolo, S ^b   Castagnaro, M ^b   Barnini, C ^a   Bargelloni, L ^b  

^a Equine Patavium Hospital   (Italy)

^b UNIVERSITY OF PADOVA   (Italy)

Author keywords

BAL; Biopsy; IL; NF k ; Recurrent airway obstruction; TLR4

Indexed keywords

CHEMOKINE; CYTOKINE; GAMMA INTERFERON; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERLEUKIN 13; INTERLEUKIN 17; INTERLEUKIN 1BETA; INTERLEUKIN 6; INTERLEUKIN 8; MESSENGER RNA; TOLL LIKE RECEPTOR 4; TRANSFORMING GROWTH FACTOR BETA1; TUMOR NECROSIS FACTOR ALPHA;

AIRWAY OBSTRUCTION; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; ASTHMA; BLOOD CELL COUNT; BRONCHOSPASM; BRONCHUS; BRONCHUS BIOPSY; BRONCHUS MUCUS; CHRONIC OBSTRUCTIVE LUNG DISEASE; CONTROLLED STUDY; CYTOLOGY; ENDOSCOPY; GENE EXPRESSION; GENETIC TRANSCRIPTION; HORSE; LUNG LAVAGE; MICROBIOLOGICAL EXAMINATION; NEUTROPHIL CHEMOTAXIS; NONHUMAN; NUCLEOTIDE SEQUENCE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RECURRENT DISEASE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION;

EQUIDAE;

BAL; BIOPSY; IL; NF-KΒ; RECURRENT AIRWAY OBSTRUCTION; TLR4;

AIRWAY OBSTRUCTION; ANIMALS; BIOPSY; BRONCHOALVEOLAR LAVAGE FLUID; HORSE DISEASES; HORSES; INFLAMMATION MEDIATORS; REAL-TIME POLYMERASE CHAIN REACTION; RECURRENCE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 84888054553     PISSN: 01652427     EISSN: 18732534     Source Type: Journal    
DOI: 10.1016/j.vetimm.2013.09.020     Document Type: Article

References (40)

1. Ainsworth D.M., Grunig G., Matychak M.B., Young J., Erb H.N., Antczak D.F. Chronic recurrent airway obstruction (heaves) in horses is characterized by a Th-1 response. Proceedings of the 2003 International Conference 2003.
2. Ainsworth D.M., Grunig G., Matychak M.B., Young J., Wagner B., Erb H.N., Antczak D.F. Recurrent airway obstruction (RAO) in horses is characterized by IFN-gamma and IL-8 production in bronchoalveolar lavage cells. Vet. Immunol. Immunopathol. 2003, 96:83-91.
3. Ainsworth D.M., Wagner B., Franchini M., Grunig G., Erb H.N., Tan J.Y. Time-dependent alterations in gene expression of interleukin-8 in the bronchial epithelium of horses with recurrent airway obstruction. Am. J. Vet. Res. 2006, 67:669-677.
4. Barnes P.J. New molecular targets for the treatment of neutrophilic diseases. J. Allergy Clin. Immunol. 2007, 119(5):1055-1062.
5. Barnes P.J. New therapies for chronic obstructive pulmonary disease. Med. Princ. Pract. 2010, 19(5):330-338.
6. Berndt A., Derksen F.J., Venta P.J., Ewart S., Yuzbasiyan-Gurkan V., Robinson N.E. Elevated amount of Toll-like receptor 4 mRNA in bronchial epithelial cells is associated with airway inflammation in horses with recurrent airway obstruction. Am. J. Physiol. Lung Cell. Mol. Physiol. 2007, 292:L936-L943.
7. Bautista M.V., Chen Y., Ivanova V.S., Rahimi M.K., Watson A.M., Rose M.C. IL-8 regulates mucin gene expression at the posttranscriptional level in lung epithelial cells. J. Immunol. 2009, 183:2159-2166.
8. Bours V., Bonizzi G., Bentires-Alj M., Bureau F., Piette J., Lekeux P., Merville M. NF-kappaB activation in response to toxical and therapeutical agents: role in inflammation and cancer treatment. Toxicology 2000, 153(1-3):27-38.
9. Bowles K.S., Beadle R.E., Mouch S., Pourciau S.S., Littlefield-Chabaud M.A., Le Blanc C., Mistric L., Fermaglich D., Horohov D.W. A novel model for equine recurrent airway obstruction. Vet. Immunol. Immunopathol. 2002, 87(3/4):385-389.
10. Brusselle G.G., Joos G.F., Bracke K.R. New insights into the immunology of chronic obstructive pulmonary disease. Lancet 2011, 378:1015-1026.
11. Chen L., Wang T., Zhang J.Y., Zhang S.F., Liu D.S., Xu D., Wang X., Chen Y.J., Wen F.Q. Toll-like receptor 4 relates to lipopolysaccharide-induced mucus hypersecretion in rat airway. Arch. Med. Res. 2009, 40(1):10-17.
12. Cordeau M.E., Joubert P., Dewachi O., Hamid Q., Lavoie J.P. IL-4, IL-5 and IFN-gamma mRNA expression in pulmonary lymphocytes in equine heaves. Vet. Immunol. Immunopathol. 2004, 97:87-96.
13. Couëtil L. Respiratory Diseases by Clinical Signs 2007, International Veterinary Information Service, Ithaca, NY. http://www.ivis.org/.
14. Couëtil L., Art T., De Moffarts B., Becker M., Melotte D., Jaspar F., Bureau F., Lekeux P. DNA binding activity of transcription factors in bronchial cells of horses with recurrent airway obstruction 2. Vet. Immunol. Immunopathol. 2006, 113:11-20.
15. Di Stefano A., Capelli A., Lusuardi M., Caramori G., Balbo P., Ioli F., Sacco S., Gnemmi I., Brun P., Adcock I.M., Balbi B., Barnes P.J., Chung K.F., Donner C.F. Decreased T lymphocyte infiltration in bronchial biopsies of subjects with severe chronic obstructive pulmonary disease. Clin. Exp. Allergy 2001, 31(6):893-902.
16. Di Stefano A., Caramori G., Ricciardolo F.L., Capelli A., Adcock I.M., Donner C.F. Cellular and molecular mechanisms in chronic obstructive pulmonary disease: an overview. Clin. Exp. Allergy 2004, 34(8):1156-1167.
17. Franchini M., Gilli U., Akens M.K., Fellenberg R.V., Bracher V. The role of neutrophil chemotactic cytokines in the pathogenesis of equine chronic obstructive pulmonary disease (COPD). Vet. Immunol. Immunopathol. 1998, 66:53-65.
18. Franchini M., Gilli U., von Fellenberg R., Bracher V.D. Interleukin-8 concentration and neutrophil chemotactic activity in bronchoalveolar lavage fluid of horses with chronic obstructive pulmonary disease following exposure to hay. Am. J. Vet. Res. 2000, 61:1369-1374.
19. Gerber V., Straub R., Marti E., Hauptman J., Herholz C., King M., Imhof A., Tahon L., Robinson N.E. Endoscopic scoring of mucus quantity and quality: observer and horse variance and relationship to inflammation, mucus viscoelasticity and volume. Equine Vet. J. 2004, 36:576-582.
20. Giguère S., Viel L., Lee E., MacKay R.J., Hernandez J., Franchini M. Cytokine induction in pulmonary airways of horses with heaves and effect of therapy with inhaled fluticasone propionate. Vet. Immunol. Immunopathol. 2002, 85(3/4):147-158.
21. Horohov D.W., Beadle R.E., Mouch S., Pourciau S.S. Temporal regulation of cytokine mRNA expression in equine recurrent airway obstruction. Vet. Immunol. Immunopathol. 2005, 108:237-245.
22. Hemmi H., Akira S. TLR signalling and the function of dendritic cells. Chem. Immunol. Allergy 2005, 86:120-135.
23. Imanifooladi A.A., Yazdani S., Nourani M.R. The role of nuclear factor-kappaB in inflammatory lung disease. Inflamm. Allergy Drug Targets 2010, 9(3):197-205.
24. Jeffery P., Holgate S., Wenzel S. Methods for the assessment of endobronchial biopsies in clinical research: application to studies of pathogenesis and the effects of treatment. Am. J. Respir. Crit. Care Med. 2003, 168:S1-S17.
25. Künzle F., Gerber V., Van der Haegen A., Wampfler B., Straub R., Marti E. IgE-bearing cells in bronchoalveolar lavage fluid and allergen-specific IgE levels in sera from RAO-affected horses. J. Vet. Med. 2007, 54(1):40-47.
26. Kuperman D.A., Huang X., Koth L.L., Chang G.H., Dolganov G.M., Zhu Z., Elias J.A., Sheppard D., Erle D.J. Direct effects of interleukin-13 on epithelial cells cause airway hyperreactivity and mucus overproduction in asthma. Nat. Med. 2002, 8:885-889.
27. Laan T.T., Bull S., Van Nieuwstadt R.A., Fink-Gremmels J. The effect of aerosolized and intravenously administered clenbuterol and aerosolized fluticasone propionate on horses challenged with Aspergillus fumigatus antigen. Vet. Res. Commun. 2006, 30(6):623-635.
28. Larocca N.E., Moreno D., Garmendia J.V., De Sanctis J.B. Inhibitors of phosphoinositol 3 kinase and NFκB for the treatment of chronic obstructive pulmonary disease. Recent Pat. Inflamm. Allergy Drug Discov. 2011, 5(3):178-183.
29. Lavoie J.P., Lefebvre-Lavoie J., Leclere M., Lavoie-Lamoureux A., Laprise C., Lussier J. Profiling of differentially expressed genes using suppression subtractive hybridization in an equine model of chronic asthma. PLoS ONE 2012, 7(1):e29440.
30. Leclere M., Lavoie-Lamoureux A., Lavoie J.P. Heaves, an asthma-like disease of horses. Respirology 2011, 16(7):1027-1046.
31. Morán G., Folch H. Recurrent airway obstruction in horses - an allergic inflammation: a review. Vet. Med. 2011, 56:1-13.
32. Ngoc P.L., Gold D.R., Tzianabos A.O., Weiss S.T., Celedón J.C. Cytokines, allergy, and asthma. Curr. Opin. Allergy Clin. Immunol. 2005, 5(2):161-166.
33. Ou X.M., Feng Y.L., Wen F.Q., Wang K., Yang J., Deng Z.P., Liu D.S., Li Y.P. Macrolides attenuate mucus hypersecretion in rat airways through inactivation of NF-kappaB. Respirology 2008, 13(1):63-72.
34. Pace E., Giarratano A., Ferraro M., Bruno A., Siena L., Mangione S., Johnson M., Gjomarkaj M. TLR4 upregulation underpins airway neutrophilia in smokers with chronic obstructive pulmonary disease and acute respiratory failure. Hum. Immunol. 2011, 72(1):54-62.
35. Riihimäki M., Raine A., Art T., Lekeux P., Couëtil L., Pringle J. Partial divergence of cytokine mRNA expression in bronchial tissues compared to bronchoalveolar lavage cells in horses with recurrent airway obstruction. Vet. Immunol. Immunopathol. 2008, 122(3/4):256-264.
36. Saunders C., Bureau F., Turlej R., Fievez L., Dogne S., Kirschvink N., Lekeux P. p65 homodyne activity distal airway cells determines lung dysfunction in equine heaves. Vet. Immunol. Immunopathol. 2001, 80:315-326.
37. Singh Suri S., Janardhan K.S., Parbhakar O., Caldwell S., Appleyard G., Singh B. Expression of toll-like receptor 4 and 2 in horse lungs. Vet. Res. 2006, 37:541-551.
38. Van Erck E., Art T., Lekeux P. Normal Respiratory Values in "Equine Respiratory Diseases 2005, International Veterinary Information Service, Ithaca, NY, (www.ivis.org).
39. Wang W., Zheng M. Nuclear factor kappa B pathway down-regulates aquaporin 5 in the nasal mucosa of rats with allergic rhinitis. Eur. Arch. Otorhinolaryngol. 2011, 268(1):73-81.
40. Zhang X., Shan P., Jiang G., Cohn L., Lee P.J. Toll-like receptor 4 deficiency causes pulmonary emphysema. J. Clin. Invest. 2006, 116:3050-3059.