메뉴 건너뛰기




Volumn 49, Issue 8, 2013, Pages 1027-1037

Analysis of soil fungal communities by amplicon pyrosequencing: Current approaches to data analysis and the introduction of the pipeline SEED

Author keywords

Fungal community; Internal transcribed spacer; Pyrosequencing pipeline; Ribosomal DNA; Sequencing errors; Soil microbial ecology

Indexed keywords

BIOGEOGRAPHY; CHIMERA; DATA ASSIMILATION; DNA; FUNGUS; MICROBIAL COMMUNITY; MICROBIAL ECOLOGY; PIPELINE; PLANT; SYMBIONT;

EID: 84888007223     PISSN: 01782762     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00374-013-0801-y     Document Type: Article
Times cited : (165)

References (63)
  • 2
    • 84862611299 scopus 로고    scopus 로고
    • Coral-associated marine fungi form novel lineages and heterogeneous assemblages
    • Amend AS, Barshis DJ, Oliver TA (2012) Coral-associated marine fungi form novel lineages and heterogeneous assemblages. ISME J 6: 1291-1301.
    • (2012) ISME J , vol.6 , pp. 1291-1301
    • Amend, A.S.1    Barshis, D.J.2    Oliver, T.A.3
  • 3
    • 78650161976 scopus 로고    scopus 로고
    • Quantifying microbial communities with 454 pyrosequencing: does read abundance count?
    • Amend AS, Seifert KA, Bruns TD (2010a) Quantifying microbial communities with 454 pyrosequencing: does read abundance count? Mol Ecol 19: 5555-5565.
    • (2010) Mol Ecol , vol.19 , pp. 5555-5565
    • Amend, A.S.1    Seifert, K.A.2    Bruns, T.D.3
  • 4
    • 77956373482 scopus 로고    scopus 로고
    • Indoor fungal composition is geographically patterned and more diverse in temperate zones than in the tropics
    • Amend AS, Seifert KA, Samson R, Bruns TD (2010b) Indoor fungal composition is geographically patterned and more diverse in temperate zones than in the tropics. Proc Nat Acad Sci U S A 107: 13748-13753.
    • (2010) Proc Nat Acad Sci U S A , vol.107 , pp. 13748-13753
    • Amend, A.S.1    Seifert, K.A.2    Samson, R.3    Bruns, T.D.4
  • 5
    • 0037224886 scopus 로고    scopus 로고
    • Potential bias of fungal 18S rDNA and internal transcribed spacer polymerase chain reaction primers for estimating fungal biodiversity in soil
    • Anderson IC, Campbell CD, Prosser JI (2003) Potential bias of fungal 18S rDNA and internal transcribed spacer polymerase chain reaction primers for estimating fungal biodiversity in soil. Environ Microbiol 5: 36-47.
    • (2003) Environ Microbiol , vol.5 , pp. 36-47
    • Anderson, I.C.1    Campbell, C.D.2    Prosser, J.I.3
  • 6
    • 84855289503 scopus 로고    scopus 로고
    • Multiple markers pyrosequencing reveals highly diverse and host-specific fungal communities on the mangrove trees Avicennia marina and Rhizophora stylosa
    • Arfi Y, Buéé M, Marchand C, Levasseur A, Record E (2012a) Multiple markers pyrosequencing reveals highly diverse and host-specific fungal communities on the mangrove trees Avicennia marina and Rhizophora stylosa. FEMS Microbiol Ecol 79: 433-444.
    • (2012) FEMS Microbiol Ecol , vol.79 , pp. 433-444
    • Arfi, Y.1    Buéé, M.2    Marchand, C.3    Levasseur, A.4    Record, E.5
  • 7
    • 84857055606 scopus 로고    scopus 로고
    • Fungal diversity in anoxic-sulfidic sediments in a mangrove soil
    • Arfi Y, Marchand C, Wartel M, Record E (2012b) Fungal diversity in anoxic-sulfidic sediments in a mangrove soil. Fungal Ecol 5: 282-285.
    • (2012) Fungal Ecol , vol.5 , pp. 282-285
    • Arfi, Y.1    Marchand, C.2    Wartel, M.3    Record, E.4
  • 10
    • 84858002835 scopus 로고    scopus 로고
    • Host identity impacts rhizosphere fungal communities associated with three alpine plant species
    • Becklin KM, Hertweck KL, Jumpponen A (2012) Host identity impacts rhizosphere fungal communities associated with three alpine plant species. Microb Ecol 63: 682-693.
    • (2012) Microb Ecol , vol.63 , pp. 682-693
    • Becklin, K.M.1    Hertweck, K.L.2    Jumpponen, A.3
  • 11
    • 77954336858 scopus 로고    scopus 로고
    • ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases
    • Bellemain E, Carlsen T, Brochmann C, Coissac E, Taberlet P, Kauserud H (2010) ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases. BMC Microbiol 10: 189.
    • (2010) BMC Microbiol , vol.10 , pp. 189
    • Bellemain, E.1    Carlsen, T.2    Brochmann, C.3    Coissac, E.4    Taberlet, P.5    Kauserud, H.6
  • 12
    • 84857508990 scopus 로고    scopus 로고
    • Metagenetic community analysis of microbial eukaryotes illuminates biogeographic patterns in deep-sea and shallow water sediments
    • Bik HM, Sung W, De Ley P, Baldwin JG, Sharma J, Rocha-Olivares A, Thomas WK (2012) Metagenetic community analysis of microbial eukaryotes illuminates biogeographic patterns in deep-sea and shallow water sediments. Mol Ecol 21: 1048-1059.
    • (2012) Mol Ecol , vol.21 , pp. 1048-1059
    • Bik, H.M.1    Sung, W.2    De Ley, P.3    Baldwin, J.G.4    Sharma, J.5    Rocha-Olivares, A.6    Thomas, W.K.7
  • 13
    • 84859574463 scopus 로고    scopus 로고
    • Changes in the root-associated fungal communities along a primary succession gradient analysed by 454 pyrosequencing
    • Blaalid R, Carlsen T, Kumar S, Halvorsen R, Ugland KI, Fontana G, Kauserud H (2012) Changes in the root-associated fungal communities along a primary succession gradient analysed by 454 pyrosequencing. Mol Ecol 21: 1897-1908.
    • (2012) Mol Ecol , vol.21 , pp. 1897-1908
    • Blaalid, R.1    Carlsen, T.2    Kumar, S.3    Halvorsen, R.4    Ugland, K.I.5    Fontana, G.6    Kauserud, H.7
  • 14
    • 70349482733 scopus 로고    scopus 로고
    • 454 Pyrosequencing analyses of forest soils reveal an unexpectedly high fungal diversity
    • Buéé M, Reich M, Murat C, Morin E, Nilsson RH, Uroz S, Martin F (2009) 454 Pyrosequencing analyses of forest soils reveal an unexpectedly high fungal diversity. New Phytol 184: 449-456.
    • (2009) New Phytol , vol.184 , pp. 449-456
    • Buéé, M.1    Reich, M.2    Murat, C.3    Morin, E.4    Nilsson, R.H.5    Uroz, S.6    Martin, F.7
  • 16
    • 84864101999 scopus 로고    scopus 로고
    • Spatial variability of phyllosphere fungal assemblages: genetic distance predominates over geographic distance in a European beech stand (Fagus syluatica)
    • Cordier T, Robin C, Capdevielle X, Desprez-Loustau ML, Vacher C (2012) Spatial variability of phyllosphere fungal assemblages: genetic distance predominates over geographic distance in a European beech stand (Fagus syluatica). Fungal Ecol 5: 509-520.
    • (2012) Fungal Ecol , vol.5 , pp. 509-520
    • Cordier, T.1    Robin, C.2    Capdevielle, X.3    Desprez-Loustau, M.L.4    Vacher, C.5
  • 17
    • 84864546249 scopus 로고    scopus 로고
    • Seasonal trends in the biomass and structure of bryophyte-associated fungal communities explored by 454 pyrosequencing
    • Davey ML, Heegaard E, Halvorsen R, Ohlson M, Kauserud H (2012) Seasonal trends in the biomass and structure of bryophyte-associated fungal communities explored by 454 pyrosequencing. New Phytol 195: 844-856.
    • (2012) New Phytol , vol.195 , pp. 844-856
    • Davey, M.L.1    Heegaard, E.2    Halvorsen, R.3    Ohlson, M.4    Kauserud, H.5
  • 18
    • 84864694016 scopus 로고    scopus 로고
    • Communities of arbuscular mycorrhizal fungi detected in forest soil are spatially heterogeneous but do not vary throughout the growing season
    • Davison J, Öpik M, Zobel M, Vasar M, Metsis M, Moora M (2012) Communities of arbuscular mycorrhizal fungi detected in forest soil are spatially heterogeneous but do not vary throughout the growing season. PLoS One 7: e41938.
    • (2012) PLoS One , vol.7
    • Davison, J.1    Öpik, M.2    Zobel, M.3    Vasar, M.4    Metsis, M.5    Moora, M.6
  • 19
  • 20
    • 77957244650 scopus 로고    scopus 로고
    • Search and clustering orders of magnitude faster than BLAST
    • Edgar RC (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26: 2460-2461.
    • (2010) Bioinformatics , vol.26 , pp. 2460-2461
    • Edgar, R.C.1
  • 22
    • 80051688000 scopus 로고    scopus 로고
    • Field guide to next-generation DNA sequencers
    • Glenn TC (2011) Field guide to next-generation DNA sequencers. Mol Ecol Res 11: 759-769.
    • (2011) Mol Ecol Res , vol.11 , pp. 759-769
    • Glenn, T.C.1
  • 25
    • 79952361581 scopus 로고    scopus 로고
    • EcM fungal community structure, but not diversity, altered in a Pb-contaminated shooting range in a boreal coniferous forest site in Southern Finland
    • Hui N, Jumpponen A, Niskanen T, Liimatainen K, Jones KL, Koivula T, Romantschuk M, Strommer R (2011) EcM fungal community structure, but not diversity, altered in a Pb-contaminated shooting range in a boreal coniferous forest site in Southern Finland. FEMS Microbiol Ecol 76: 121-132.
    • (2011) FEMS Microbiol Ecol , vol.76 , pp. 121-132
    • Hui, N.1    Jumpponen, A.2    Niskanen, T.3    Liimatainen, K.4    Jones, K.L.5    Koivula, T.6    Romantschuk, M.7    Strommer, R.8
  • 27
    • 70349493057 scopus 로고    scopus 로고
    • Massively parallel 454 sequencing indicates hyperdiverse fungal communities in temperate Quercus macrocarpa phyllosphere
    • Jumpponen A, Jones KL (2009) Massively parallel 454 sequencing indicates hyperdiverse fungal communities in temperate Quercus macrocarpa phyllosphere. New Phytol 184: 438-448.
    • (2009) New Phytol , vol.184 , pp. 438-448
    • Jumpponen, A.1    Jones, K.L.2
  • 28
    • 77954695245 scopus 로고    scopus 로고
    • Seasonally dynamic fungal communities in the Quercus macrocarpa phyllosphere differ between urban and nonurban environments
    • Jumpponen A, Jones KL (2010) Seasonally dynamic fungal communities in the Quercus macrocarpa phyllosphere differ between urban and nonurban environments. New Phytol 186: 496-513.
    • (2010) New Phytol , vol.186 , pp. 496-513
    • Jumpponen, A.1    Jones, K.L.2
  • 29
    • 77956640186 scopus 로고    scopus 로고
    • Vertical distribution of fungal communities in tallgrass prairie soil
    • Jumpponen A, Jones KL, Blair J (2010a) Vertical distribution of fungal communities in tallgrass prairie soil. Mycologia 102: 1027-1041.
    • (2010) Mycologia , vol.102 , pp. 1027-1041
    • Jumpponen, A.1    Jones, K.L.2    Blair, J.3
  • 30
    • 77951924594 scopus 로고    scopus 로고
    • Massively parallel 454-sequencing of fungal communities in Quercus spp. ectomycorrhizas indicates seasonal dynamics in urban and rural sites
    • Jumpponen A, Jones KL, Mattox D, Yaege C (2010b) Massively parallel 454-sequencing of fungal communities in Quercus spp. ectomycorrhizas indicates seasonal dynamics in urban and rural sites. Mol Ecol 19: 41-53.
    • (2010) Mol Ecol , vol.19 , pp. 41-53
    • Jumpponen, A.1    Jones, K.L.2    Mattox, D.3    Yaege, C.4
  • 31
    • 68049142320 scopus 로고    scopus 로고
    • Multiple alignment of DNA sequences with MAFFT
    • D. Posada (Ed.), Totowa: Humana
    • Katoh K, Asimenos G, Toh H (2009) Multiple alignment of DNA sequences with MAFFT. In: Posada D (ed) Bioinformatics for DNA sequence analysis. Humana, Totowa, pp 39-64.
    • (2009) Bioinformatics for DNA Sequence Analysis , pp. 39-64
    • Katoh, K.1    Asimenos, G.2    Toh, H.3
  • 32
    • 84859829470 scopus 로고    scopus 로고
    • High consistency between replicate 454 pyrosequencing analyses of ectomycorrhizal plant root samples
    • Kauserud H, Kumar S, Brysting AK, Nordén J, Carlsen T (2012) High consistency between replicate 454 pyrosequencing analyses of ectomycorrhizal plant root samples. Mycorrhiza 22: 309-315.
    • (2012) Mycorrhiza , vol.22 , pp. 309-315
    • Kauserud, H.1    Kumar, S.2    Brysting, A.K.3    Nordén, J.4    Carlsen, T.5
  • 33
    • 84857438893 scopus 로고    scopus 로고
    • Diversity measures in environmental sequences are highly dependent on alignment quality-data from ITS and new LSU primers targeting basidiomycetes
    • Krüger D, Kapturska D, Fischer C, Daniel R, Wubet T (2012) Diversity measures in environmental sequences are highly dependent on alignment quality-data from ITS and new LSU primers targeting basidiomycetes. PLoS One 7: e32139.
    • (2012) PLoS One , vol.7
    • Krüger, D.1    Kapturska, D.2    Fischer, C.3    Daniel, R.4    Wubet, T.5
  • 34
    • 84866234470 scopus 로고    scopus 로고
    • Patterns of fungal communities among and within decaying logs, revealed by 454 sequencing
    • Kubartova A, Ottosson E, Dahlberg A, Stenlid J (2012) Patterns of fungal communities among and within decaying logs, revealed by 454 sequencing. Mol Ecol 21: 4514-4532.
    • (2012) Mol Ecol , vol.21 , pp. 4514-4532
    • Kubartova, A.1    Ottosson, E.2    Dahlberg, A.3    Stenlid, J.4
  • 35
    • 84866172403 scopus 로고    scopus 로고
    • Pyrosequencing-derived bacterial, archaeal, and fungal diversity of spacecraft hardware destined for Mars
    • La Duc MT, Vaishampayan P, Nilsson HR, Torok T, Venkateswaran K (2012) Pyrosequencing-derived bacterial, archaeal, and fungal diversity of spacecraft hardware destined for Mars. Appl Environ Microbiol 78: 5912-5922.
    • (2012) Appl Environ Microbiol , vol.78 , pp. 5912-5922
    • La Duc, M.T.1    Vaishampayan, P.2    Nilsson, H.R.3    Torok, T.4    Venkateswaran, K.5
  • 36
    • 83455224377 scopus 로고    scopus 로고
    • 454-Sequencing reveals stochastic local reassembly and high disturbance tolerance within arbuscular mycorrhizal fungal communities
    • Lekberg Y, Schnoor T, Kjøller R, Gibbons SM, Hansen LH, Al-Soud WA, Sorensen SJ, Rosendahl S (2012) 454-Sequencing reveals stochastic local reassembly and high disturbance tolerance within arbuscular mycorrhizal fungal communities. J Ecol 100: 151-160.
    • (2012) J Ecol , vol.100 , pp. 151-160
    • Lekberg, Y.1    Schnoor, T.2    Kjøller, R.3    Gibbons, S.M.4    Hansen, L.H.5    Al-Soud, W.A.6    Sorensen, S.J.7    Rosendahl, S.8
  • 38
    • 84861871568 scopus 로고    scopus 로고
    • Long-term balanced fertilization decreases arbuscular mycorrhizal fungal diversity in an arable soil in North China revealed by 454 pyrosequencing
    • Lin XG, Feng YZ, Zhang HY, Chen RR, Wang JH, Zhang JB, Chu HY (2012) Long-term balanced fertilization decreases arbuscular mycorrhizal fungal diversity in an arable soil in North China revealed by 454 pyrosequencing. Environ Sci Technol 46: 5764-5771.
    • (2012) Environ Sci Technol , vol.46 , pp. 5764-5771
    • Lin, X.G.1    Feng, Y.Z.2    Zhang, H.Y.3    Chen, R.R.4    Wang, J.H.5    Zhang, J.B.6    Chu, H.Y.7
  • 39
    • 77955262221 scopus 로고    scopus 로고
    • Disclosing arbuscular mycorrhizal fungal biodiversity in soil through a land-use gradient using a pyrosequencing approach
    • Lumini E, Orgiazzi A, Borriello R, Bonfante P, Bianciotto V (2010) Disclosing arbuscular mycorrhizal fungal biodiversity in soil through a land-use gradient using a pyrosequencing approach. Environ Microbiol 12: 2165-2179.
    • (2010) Environ Microbiol , vol.12 , pp. 2165-2179
    • Lumini, E.1    Orgiazzi, A.2    Borriello, R.3    Bonfante, P.4    Bianciotto, V.5
  • 40
    • 84864363325 scopus 로고    scopus 로고
    • Fungal community composition in neotropical rain forests: the influence of tree diversity and precipitation
    • McGuire KL, Fierer N, Bateman C, Treseder KK, Turner BL (2012) Fungal community composition in neotropical rain forests: the influence of tree diversity and precipitation. Microb Ecol 63: 804-812.
    • (2012) Microb Ecol , vol.63 , pp. 804-812
    • McGuire, K.L.1    Fierer, N.2    Bateman, C.3    Treseder, K.K.4    Turner, B.L.5
  • 41
    • 82455175364 scopus 로고    scopus 로고
    • ITS-1 versus ITS-2 pyrosequencing: a comparison of fungal populations in truffle grounds
    • Mello A, Napoli C, Murat C, Morin E, Marceddu G, Bonfante P (2011) ITS-1 versus ITS-2 pyrosequencing: a comparison of fungal populations in truffle grounds. Mycologia 103: 1184-1193.
    • (2011) Mycologia , vol.103 , pp. 1184-1193
    • Mello, A.1    Napoli, C.2    Murat, C.3    Morin, E.4    Marceddu, G.5    Bonfante, P.6
  • 45
    • 77956292565 scopus 로고    scopus 로고
    • An open source software package for automated extraction of ITS1 and ITS2 from fungal ITS sequences for use in high-throughput community assays and molecular ecology
    • Nilsson RH, Veldre V, Hartmann M, Unterseher M, Amend A, Bergsten J, Kristiansson E, Ryberg M, Jumpponen A, Abarenkov K (2010) An open source software package for automated extraction of ITS1 and ITS2 from fungal ITS sequences for use in high-throughput community assays and molecular ecology. Fungal Ecol 3: 284-287.
    • (2010) Fungal Ecol , vol.3 , pp. 284-287
    • Nilsson, R.H.1    Veldre, V.2    Hartmann, M.3    Unterseher, M.4    Amend, A.5    Bergsten, J.6    Kristiansson, E.7    Ryberg, M.8    Jumpponen, A.9    Abarenkov, K.10
  • 46
    • 70349525156 scopus 로고    scopus 로고
    • Large-scale parallel 454 sequencing reveals host ecological group specificity of arbuscular mycorrhizal fungi in a boreonemoral forest
    • Öpik M, Metsis M, Daniell TJ, Zobel M, Moora M (2009) Large-scale parallel 454 sequencing reveals host ecological group specificity of arbuscular mycorrhizal fungi in a boreonemoral forest. New Phytol 184: 424-437.
    • (2009) New Phytol , vol.184 , pp. 424-437
    • Öpik, M.1    Metsis, M.2    Daniell, T.J.3    Zobel, M.4    Moora, M.5
  • 48
    • 84866004889 scopus 로고    scopus 로고
    • A better understanding of functional roles of fungi in the decomposition process: using precursor rRNA containing ITS regions as a marker for the active fungal community
    • Purahong W, Krüger D (2012) A better understanding of functional roles of fungi in the decomposition process: using precursor rRNA containing ITS regions as a marker for the active fungal community. Ann Forest Sci 69: 659-662.
    • (2012) Ann Forest Sci , vol.69 , pp. 659-662
    • Purahong, W.1    Krüger, D.2
  • 51
    • 84859625932 scopus 로고    scopus 로고
    • Next-generation sequencing technologies for environmental DNA research
    • Shokralla S, Spall JL, Gibson JF, Hajibabaei M (2012) Next-generation sequencing technologies for environmental DNA research. Mol Ecol 21: 1794-1805.
    • (2012) Mol Ecol , vol.21 , pp. 1794-1805
    • Shokralla, S.1    Spall, J.L.2    Gibson, J.F.3    Hajibabaei, M.4
  • 54
    • 84860692234 scopus 로고    scopus 로고
    • Cellulose utilization in forest litter and soil: identification of bacterial and fungal decomposers
    • Štursová M, Žifčáková L, Leigh MB, Burgess R, Baldrian P (2012) Cellulose utilization in forest litter and soil: identification of bacterial and fungal decomposers. FEMS Microbiol Ecol 80: 735-746.
    • (2012) FEMS Microbiol Ecol , vol.80 , pp. 735-746
    • Štursová, M.1    Žifčáková, L.2    Leigh, M.B.3    Burgess, R.4    Baldrian, P.5
  • 55
    • 80053114033 scopus 로고    scopus 로고
    • A bioinformatics pipeline for sequence-based analyses of fungal biodiversity
    • J. R. Xu and B. H. Bluhm (Eds.), Totowa: Humana
    • Taylor DL, Houston S (2011) A bioinformatics pipeline for sequence-based analyses of fungal biodiversity. In: Xu JR, Bluhm BH (eds) Fungal genomics. Humana, Totowa, pp 141-155.
    • (2011) Fungal Genomics , pp. 141-155
    • Taylor, D.L.1    Houston, S.2
  • 56
    • 77956283658 scopus 로고    scopus 로고
    • 454 Pyrosequencing and Sanger sequencing of tropical mycorrhizal fungi provide similar results but reveal substantial methodological biases
    • Tedersoo L, Nilsson RH, Abarenkov K, Jairus T, Sadam A, Saar I, Bahram M, Bechem E, Chuyong G, Koljalg U (2010) 454 Pyrosequencing and Sanger sequencing of tropical mycorrhizal fungi provide similar results but reveal substantial methodological biases. New Phytol 188: 291-301.
    • (2010) New Phytol , vol.188 , pp. 291-301
    • Tedersoo, L.1    Nilsson, R.H.2    Abarenkov, K.3    Jairus, T.4    Sadam, A.5    Saar, I.6    Bahram, M.7    Bechem, E.8    Chuyong, G.9    Koljalg, U.10
  • 57
    • 84874536596 scopus 로고    scopus 로고
    • The variability of the 16S rRNA gene in bacterial genomes and its consequences for bacterial community analyses
    • Větrovský T, Baldrian P (2013) The variability of the 16S rRNA gene in bacterial genomes and its consequences for bacterial community analyses. PLoS One 8: e57923.
    • (2013) PLoS One , vol.8
    • Větrovský, T.1    Baldrian, P.2
  • 58
    • 84875162399 scopus 로고    scopus 로고
    • Fungal community on decomposing leaf litter undergoes rapid successional changes
    • Voříšková J, Baldrian P (2013) Fungal community on decomposing leaf litter undergoes rapid successional changes. ISME J 7: 477-486.
    • (2013) ISME J , vol.7 , pp. 477-486
    • Voříšková, J.1    Baldrian, P.2
  • 59
    • 77955777197 scopus 로고    scopus 로고
    • Production of ectomycorrhizal mycelium peaks during canopy closure in Norway spruce forests
    • Wallander H, Johansson U, Sterkenburg E, Durling MB, Lindahl BD (2010) Production of ectomycorrhizal mycelium peaks during canopy closure in Norway spruce forests. New Phytol 187: 1124-1134.
    • (2010) New Phytol , vol.187 , pp. 1124-1134
    • Wallander, H.1    Johansson, U.2    Sterkenburg, E.3    Durling, M.B.4    Lindahl, B.D.5
  • 61
    • 83055163369 scopus 로고    scopus 로고
    • Influence of DNA extraction and PCR amplification on studies of soil fungal communities based on amplicon sequencing
    • Xu LH, Ravnskov S, Larsen J, Nicolaisen M (2011) Influence of DNA extraction and PCR amplification on studies of soil fungal communities based on amplicon sequencing. Can J Microbiol 57: 1062-1066.
    • (2011) Can J Microbiol , vol.57 , pp. 1062-1066
    • Xu, L.H.1    Ravnskov, S.2    Larsen, J.3    Nicolaisen, M.4
  • 62
    • 84864143923 scopus 로고    scopus 로고
    • Molecular characterization of root-associated fungal communities in relation to health status of Pisum sativum using barcoded pyrosequencing
    • Yu L, Nicolaisen M, Larsen J, Ravnskov S (2012a) Molecular characterization of root-associated fungal communities in relation to health status of Pisum sativum using barcoded pyrosequencing. Plant Soil 357: 395-405.
    • (2012) Plant Soil , vol.357 , pp. 395-405
    • Yu, L.1    Nicolaisen, M.2    Larsen, J.3    Ravnskov, S.4
  • 63
    • 84865736246 scopus 로고    scopus 로고
    • Succession of root-associated fungi in Pisum sativum during a plant growth cycle as examined by 454 pyrosequencing
    • Yu L, Nicolaisen M, Larsen J, Ravnskov S (2012b) Succession of root-associated fungi in Pisum sativum during a plant growth cycle as examined by 454 pyrosequencing. Plant Soil 358: 216-224.
    • (2012) Plant Soil , vol.358 , pp. 216-224
    • Yu, L.1    Nicolaisen, M.2    Larsen, J.3    Ravnskov, S.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.