Development of phenotypic screening assays for γ-globin induction using primary human bone marrow day 7 erythroid progenitor cells

Journal of Biomolecular Screening

Volumn 18, Issue 10, 2013, Pages 1212-1222

  Li, Hu ^a   Xie, Wensheng ^a   Gore, Elizabeth R ^a   Montoute, Monica N ^a   Bee, Weilin Tiger ^a   Zappacosta, Francesca ^a   Zeng, Xin ^a   Wu, Zining ^a   Kallal, Lorena ^a   Ames, Robert S ^a   Pope, Andrew J ^a   Benowitz, Andrew ^a   Erickson Miller, Connie L ^a  

^a GLAXOSMITHKLINE   (United States)

Author keywords

globin; decitabine; ELISA; fetal hemoglobin (HbF); hydroxyurea (HU); phenotypic screen; sickle cell anemia

Indexed keywords

5 AZA 2' DEOXYCYTIDINE; ANTIGEN; CD235A ANTIGEN; CD36 ANTIGEN; CD71 ANTIGEN; HEMOGLOBIN GAMMA CHAIN; HYDROXYUREA; UNCLASSIFIED DRUG;

ARTICLE; CELL EXPANSION; CONTROLLED STUDY; DRUG SCREENING; ENZYME LINKED IMMUNOSORBENT ASSAY; ERYTHROID PRECURSOR CELL; GENE INDUCTION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS;

DECITABINE; ELISA; FETAL HEMOGLOBIN (HBF); HYDROXYUREA (HU); PHENOTYPIC SCREEN; SICKLE CELL ANEMIA; Γ-GLOBIN;

ANEMIA, SICKLE CELL; AZACITIDINE; BONE MARROW CELLS; BUTYRIC ACID; CELL SURVIVAL; DNA MODIFICATION METHYLASES; DRUG EVALUATION, PRECLINICAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPIGENESIS, GENETIC; ERYTHROID PRECURSOR CELLS; GAMMA-GLOBINS; HISTONE DEACETYLASE INHIBITORS; HUMANS; PRIMARY CELL CULTURE; TRANSCRIPTIONAL ACTIVATION;

EID: 84887905876     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057113499776     Document Type: Article

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