메뉴 건너뛰기




Volumn 12, Issue 1, 2013, Pages

A low-cost, multiplexable, automated flow cytometry procedure for the characterization of microbial stress dynamics in bioreactors

Author keywords

Automated flow cytometry; Escherichia coli; Green fluorescent protein; Membrane permeability; Mini bioreactors; Phenotypic heterogeneity

Indexed keywords

ADENOSINE TRIPHOSPHATE; GREEN FLUORESCENT PROTEIN; PROPIDIUM IODIDE; PROTEINASE;

EID: 84887895265     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-12-100     Document Type: Article
Times cited : (46)

References (44)
  • 1
    • 77950627389 scopus 로고    scopus 로고
    • Origin and analysis of microbial population heterogeneity in bioprocesses
    • 10.1016/j.copbio.2010.01.002, 20138500
    • Müller S, Harms H, Bley T. Origin and analysis of microbial population heterogeneity in bioprocesses. Curr Opin Biotechnol 2010, 21:100-113. 10.1016/j.copbio.2010.01.002, 20138500.
    • (2010) Curr Opin Biotechnol , vol.21 , pp. 100-113
    • Müller, S.1    Harms, H.2    Bley, T.3
  • 2
    • 0037078982 scopus 로고    scopus 로고
    • Control, exploitation and tolerance of intracellular noise
    • 10.1038/nature01258, 12432408
    • Rao CV, Wolf DM, Arkin AP. Control, exploitation and tolerance of intracellular noise. Nature 2002, 420:231-237. 10.1038/nature01258, 12432408.
    • (2002) Nature , vol.420 , pp. 231-237
    • Rao, C.V.1    Wolf, D.M.2    Arkin, A.P.3
  • 3
    • 33646540711 scopus 로고    scopus 로고
    • Predicting stochastic gene expression dynamics in single cells
    • 10.1073/pnas.0509874103, 1464336, 16648266
    • Mettetal JT, Muzzey D, Pedraza JM, Ozbudak EM, van Oudenaarden A. Predicting stochastic gene expression dynamics in single cells. Proc Natl Acad Sci U S A 2006, 103:7304-7309. 10.1073/pnas.0509874103, 1464336, 16648266.
    • (2006) Proc Natl Acad Sci U S A , vol.103 , pp. 7304-7309
    • Mettetal, J.T.1    Muzzey, D.2    Pedraza, J.M.3    Ozbudak, E.M.4    van Oudenaarden, A.5
  • 4
    • 2942555282 scopus 로고    scopus 로고
    • Stochastic gene expression in fluctuating environments
    • 10.1534/genetics.167.1.523, 1470854, 15166174
    • Thattai M, van Oudenaarden A. Stochastic gene expression in fluctuating environments. Genetics 2004, 167:523-530. 10.1534/genetics.167.1.523, 1470854, 15166174.
    • (2004) Genetics , vol.167 , pp. 523-530
    • Thattai, M.1    van Oudenaarden, A.2
  • 5
    • 65249187631 scopus 로고    scopus 로고
    • External, extrinsic and intrinsic noise in cellular systems : analogies and implications for protein synthesis
    • Patnaik PR. External, extrinsic and intrinsic noise in cellular systems : analogies and implications for protein synthesis. Biotechnol Mol Biol Rev 2006, 1:121-127.
    • (2006) Biotechnol Mol Biol Rev , vol.1 , pp. 121-127
    • Patnaik, P.R.1
  • 6
    • 75349086967 scopus 로고    scopus 로고
    • Application of flow cytometry to industrial microbial bioprocesses
    • Diaz M, Herrero M, Garcia LA, Quiros C. Application of flow cytometry to industrial microbial bioprocesses. Biochem Eng J 2010, 48:385-407.
    • (2010) Biochem Eng J , vol.48 , pp. 385-407
    • Diaz, M.1    Herrero, M.2    Garcia, L.A.3    Quiros, C.4
  • 7
    • 84862006007 scopus 로고    scopus 로고
    • Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity
    • 10.1371/journal.pone.0037915, 3369916, 22685548
    • Love KR, Politano TJ, Panagiotou V, Jiang B, Stadheim TA, Love JC. Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity. PLoS One 2012, 7:e37915. 10.1371/journal.pone.0037915, 3369916, 22685548.
    • (2012) PLoS One , vol.7
    • Love, K.R.1    Politano, T.J.2    Panagiotou, V.3    Jiang, B.4    Stadheim, T.A.5    Love, J.C.6
  • 8
    • 0033525428 scopus 로고    scopus 로고
    • A flow injection flow cytometry system for on-line monitoring of bioreactors
    • 10.1002/(SICI)1097-0290(19990305)62:5<609::AID-BIT13>3.0.CO;2-C, 10099570
    • Zhao R, Natarajan A, Srienc F. A flow injection flow cytometry system for on-line monitoring of bioreactors. Biotechnol Bioeng 1999, 62:609-617. 10.1002/(SICI)1097-0290(19990305)62:5<609::AID-BIT13>3.0.CO;2-C, 10099570.
    • (1999) Biotechnol Bioeng , vol.62 , pp. 609-617
    • Zhao, R.1    Natarajan, A.2    Srienc, F.3
  • 9
    • 0142058417 scopus 로고    scopus 로고
    • Automated flow cytometry for acquisition of time-dependent population data
    • Abu-Absi NR, Zamamiri A, Kacmar J, Balogh SJ, Srienc F. Automated flow cytometry for acquisition of time-dependent population data. Cytometry A 2003, 51A:87-96.
    • (2003) Cytometry A , vol.51 A , pp. 87-96
    • Abu-Absi, N.R.1    Zamamiri, A.2    Kacmar, J.3    Balogh, S.J.4    Srienc, F.5
  • 11
    • 0033527396 scopus 로고    scopus 로고
    • Monitoring GFP operon fusion protein expression during high cell density cultivation of Escherichia coli using an on-line optical sensor
    • 10.1002/(SICI)1097-0290(19991005)65:1<54::AID-BIT7>3.0.CO;2-R, 10440671
    • DeLisa MP, Li J, Rao G, Weigand WA, Bentley WE. Monitoring GFP operon fusion protein expression during high cell density cultivation of Escherichia coli using an on-line optical sensor. Biotechnol Bioeng 1999, 65:54-64. 10.1002/(SICI)1097-0290(19991005)65:1<54::AID-BIT7>3.0.CO;2-R, 10440671.
    • (1999) Biotechnol Bioeng , vol.65 , pp. 54-64
    • DeLisa, M.P.1    Li, J.2    Rao, G.3    Weigand, W.A.4    Bentley, W.E.5
  • 12
    • 33749623925 scopus 로고    scopus 로고
    • The cytostat: A new way to study cell physiology in a precisely defined environment
    • 10.1016/j.jbiotec.2006.04.015, 16716427
    • Kacmar J, Gilbert A, Cockrell J, Srienc F. The cytostat: A new way to study cell physiology in a precisely defined environment. J Biotechnol 2006, 126:163-172. 10.1016/j.jbiotec.2006.04.015, 16716427.
    • (2006) J Biotechnol , vol.126 , pp. 163-172
    • Kacmar, J.1    Gilbert, A.2    Cockrell, J.3    Srienc, F.4
  • 13
    • 84868638780 scopus 로고    scopus 로고
    • Automated processing integrated with a microflow cytometer for pathogen detection in clinical matrices
    • 10.1016/j.bios.2012.08.015, 22960010
    • Golden JP, Verbarg J, Howell PB, Shriver-Lake LC, Ligler FS. Automated processing integrated with a microflow cytometer for pathogen detection in clinical matrices. Biosens Bioelectron 2013, 40:10-16. 10.1016/j.bios.2012.08.015, 22960010.
    • (2013) Biosens Bioelectron , vol.40 , pp. 10-16
    • Golden, J.P.1    Verbarg, J.2    Howell, P.B.3    Shriver-Lake, L.C.4    Ligler, F.S.5
  • 14
    • 84878831208 scopus 로고    scopus 로고
    • Routine bacterial analysis with automated flow cytometry
    • 10.1016/j.mimet.2013.05.007, 23684992
    • Van Nevel S, Koetzsch S, Weilenmann HU, Boon N, Hammes F. Routine bacterial analysis with automated flow cytometry. J Microbiol Methods 2013, 94:73-76. 10.1016/j.mimet.2013.05.007, 23684992.
    • (2013) J Microbiol Methods , vol.94 , pp. 73-76
    • Van Nevel, S.1    Koetzsch, S.2    Weilenmann, H.U.3    Boon, N.4    Hammes, F.5
  • 15
    • 84860482344 scopus 로고    scopus 로고
    • Automated flow cytometry for monitoring CHO cell cultures
    • 10.1016/j.ymeth.2012.03.001, 22445707
    • Kuystermans D, Mohd A, Al-Rubeai M. Automated flow cytometry for monitoring CHO cell cultures. Methods 2012, 56:358-365. 10.1016/j.ymeth.2012.03.001, 22445707.
    • (2012) Methods , vol.56 , pp. 358-365
    • Kuystermans, D.1    Mohd, A.2    Al-Rubeai, M.3
  • 17
    • 79960427553 scopus 로고    scopus 로고
    • Real-time on-line flow cytometry for bioprocess monitoring
    • 10.1016/j.jbiotec.2011.05.003, 21609740
    • Broger T, Odermatt RP, Huber P, Sonnleitner B. Real-time on-line flow cytometry for bioprocess monitoring. J Biotechnol 2011, 154:240-247. 10.1016/j.jbiotec.2011.05.003, 21609740.
    • (2011) J Biotechnol , vol.154 , pp. 240-247
    • Broger, T.1    Odermatt, R.P.2    Huber, P.3    Sonnleitner, B.4
  • 18
    • 84875956222 scopus 로고    scopus 로고
    • Design of growth-dependent biosensors based on destabilized GFP for the detection of physiological behavior of Escherichia coli in heterogeneous bioreactors
    • 10.1002/btpr.1694, 23335499
    • Han S, Delvigne F, Brognaux A, Gitte C, Sorensen JS. Design of growth-dependent biosensors based on destabilized GFP for the detection of physiological behavior of Escherichia coli in heterogeneous bioreactors. Biotechnol Prog 2013, 29:553-563. 10.1002/btpr.1694, 23335499.
    • (2013) Biotechnol Prog , vol.29 , pp. 553-563
    • Han, S.1    Delvigne, F.2    Brognaux, A.3    Gitte, C.4    Sorensen, J.S.5
  • 19
    • 0026620949 scopus 로고
    • Dramatic change of fis level upon nutrient upshift in Escherichia coli
    • 207543, 1459953
    • Ball CA, Osuna R, Ferguson KC, Johnson RC. Dramatic change of fis level upon nutrient upshift in Escherichia coli. J Bacteriol 1992, 174:8043-8056. 207543, 1459953.
    • (1992) J Bacteriol , vol.174 , pp. 8043-8056
    • Ball, C.A.1    Osuna, R.2    Ferguson, K.C.3    Johnson, R.C.4
  • 21
    • 0031746834 scopus 로고    scopus 로고
    • New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria
    • 106306, 9603842
    • Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S. New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria. Appl Environ Microbiol 1998, 64:2240-2246. 106306, 9603842.
    • (1998) Appl Environ Microbiol , vol.64 , pp. 2240-2246
    • Andersen, J.B.1    Sternberg, C.2    Poulsen, L.K.3    Bjorn, S.P.4    Givskov, M.5    Molin, S.6
  • 22
    • 10344265937 scopus 로고    scopus 로고
    • Control of rRNA Synthesis in Escherichia coli: a Systems Biology Approach
    • 10.1128/MMBR.68.4.639-668.2004, 539008, 15590778
    • Dennis PP, Ehrenberg M, Bremer H. Control of rRNA Synthesis in Escherichia coli: a Systems Biology Approach. Microbiol Mol Biol Rev 2004, 68:639-668. 10.1128/MMBR.68.4.639-668.2004, 539008, 15590778.
    • (2004) Microbiol Mol Biol Rev , vol.68 , pp. 639-668
    • Dennis, P.P.1    Ehrenberg, M.2    Bremer, H.3
  • 23
    • 79961175327 scopus 로고    scopus 로고
    • Green Fluorescent Protein (GFP) leakage from microbial biosensors provides useful information for the estimation of the scale-down effect
    • 10.1002/biot.201000410, 21695786
    • Delvigne F, Brognaux A, Francis F, Twizere JC, Gorret N, Sorensen SJ, Thonart P. Green Fluorescent Protein (GFP) leakage from microbial biosensors provides useful information for the estimation of the scale-down effect. Biotechnol J 2011, 6:968-978. 10.1002/biot.201000410, 21695786.
    • (2011) Biotechnol J , vol.6 , pp. 968-978
    • Delvigne, F.1    Brognaux, A.2    Francis, F.3    Twizere, J.C.4    Gorret, N.5    Sorensen, S.J.6    Thonart, P.7
  • 24
    • 84873288059 scopus 로고    scopus 로고
    • Direct and indirect use of GFP whole cell biosensors for the assessment of bioprocess performances: Design of milliliter scale-down bioreactors
    • Brognaux A, Neubauer P, Twizere JC, Francis F, Gorret N, Thonart P, Delvigne F. Direct and indirect use of GFP whole cell biosensors for the assessment of bioprocess performances: Design of milliliter scale-down bioreactors. Biotechnol Prog 2012, 29:48-59.
    • (2012) Biotechnol Prog , vol.29 , pp. 48-59
    • Brognaux, A.1    Neubauer, P.2    Twizere, J.C.3    Francis, F.4    Gorret, N.5    Thonart, P.6    Delvigne, F.7
  • 25
    • 0036192267 scopus 로고    scopus 로고
    • Growth rate dependent changes in Escherichia coli membrane structure and protein leakage
    • 10.1007/s00253-001-0889-0, 11935192
    • Shokri A, Sanden AM, Larsson G. Growth rate dependent changes in Escherichia coli membrane structure and protein leakage. Appl Microbiol Biotechnol 2002, 58:386-392. 10.1007/s00253-001-0889-0, 11935192.
    • (2002) Appl Microbiol Biotechnol , vol.58 , pp. 386-392
    • Shokri, A.1    Sanden, A.M.2    Larsson, G.3
  • 26
    • 47249110176 scopus 로고    scopus 로고
    • Fedbatch design for periplasmic product retention in Escherichia coli
    • 10.1016/j.jbiotec.2008.05.002, 18579250
    • Bäcklund E, Reeks D, Marklanda K, Weir N, Bowering L, Larsson G. Fedbatch design for periplasmic product retention in Escherichia coli. J Biotechnol 2008, 135:358-365. 10.1016/j.jbiotec.2008.05.002, 18579250.
    • (2008) J Biotechnol , vol.135 , pp. 358-365
    • Bäcklund, E.1    Reeks, D.2    Marklanda, K.3    Weir, N.4    Bowering, L.5    Larsson, G.6
  • 27
    • 34948885665 scopus 로고    scopus 로고
    • Effects of Fis on Escherichia coli gene expression during different growth stages
    • 10.1099/mic.0.2007/008565-0, 17768236
    • Bradley MD, Beach MB, de Koning AP J, Pratt TS, Osuna R. Effects of Fis on Escherichia coli gene expression during different growth stages. Microbiology 2007, 153:2922-2940. 10.1099/mic.0.2007/008565-0, 17768236.
    • (2007) Microbiology , vol.153 , pp. 2922-2940
    • Bradley, M.D.1    Beach, M.B.2    de Koning, A.P.J.3    Pratt, T.S.4    Osuna, R.5
  • 28
    • 26844579270 scopus 로고    scopus 로고
    • Transient increase of ATP as a response to temperature up-shift in Escherichia coli
    • 10.1186/1475-2859-4-9, 1087501, 15804347
    • Soini J, Falschlehner C, Mayer C, Böhm D, Weinel S, Panula J, Vasala A, Neubauer P. Transient increase of ATP as a response to temperature up-shift in Escherichia coli. Microb Cell Fact 2005, 4:9. 10.1186/1475-2859-4-9, 1087501, 15804347.
    • (2005) Microb Cell Fact , vol.4 , pp. 9
    • Soini, J.1    Falschlehner, C.2    Mayer, C.3    Böhm, D.4    Weinel, S.5    Panula, J.6    Vasala, A.7    Neubauer, P.8
  • 29
    • 77957296792 scopus 로고    scopus 로고
    • How to live at very low substrate concentrations
    • Egli T. How to live at very low substrate concentrations. Water Res 2011, 44:4826-4837.
    • (2011) Water Res , vol.44 , pp. 4826-4837
    • Egli, T.1
  • 30
    • 0030200309 scopus 로고    scopus 로고
    • Adaptation to life at micromolar nutrient levels : the regulation of Escherichia coli glucose transport by endoinduction and cAMP
    • 10.1111/j.1574-6976.1996.tb00246.x, 8703508
    • Ferenci T. Adaptation to life at micromolar nutrient levels : the regulation of Escherichia coli glucose transport by endoinduction and cAMP. FEMS Microbiol Rev 1996, 18:301-317. 10.1111/j.1574-6976.1996.tb00246.x, 8703508.
    • (1996) FEMS Microbiol Rev , vol.18 , pp. 301-317
    • Ferenci, T.1
  • 31
    • 34249702940 scopus 로고    scopus 로고
    • Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLigth kit in combination with flow cytometry
    • Berney M, Hames F, Bosshard F, Weilenmann HU, Egli T. Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLigth kit in combination with flow cytometry. Appl Environ Microbiol 2007, 70:3283-3290.
    • (2007) Appl Environ Microbiol , vol.70 , pp. 3283-3290
    • Berney, M.1    Hames, F.2    Bosshard, F.3    Weilenmann, H.U.4    Egli, T.5
  • 32
    • 17144369910 scopus 로고    scopus 로고
    • Parallel reactor systems for bioprocess development
    • Weuster-Botz D. Parallel reactor systems for bioprocess development. Adv Biochem Eng Biotechnol 2005, 92:125-143.
    • (2005) Adv Biochem Eng Biotechnol , vol.92 , pp. 125-143
    • Weuster-Botz, D.1
  • 33
    • 33746640411 scopus 로고    scopus 로고
    • Miniature bioreactors : current practices and future opportunities
    • 10.1186/1475-2859-5-21, 1523360, 16725043
    • Betts JI, Baganz F. Miniature bioreactors : current practices and future opportunities. Microb Cell Fact 2006, 5:21. 10.1186/1475-2859-5-21, 1523360, 16725043.
    • (2006) Microb Cell Fact , vol.5 , pp. 21
    • Betts, J.I.1    Baganz, F.2
  • 34
    • 0036333939 scopus 로고    scopus 로고
    • Destabilized green fluorescent protein for monitoring transient changes in mycobacterial gene expression
    • 10.1016/S0923-2508(02)01327-X, 12234013
    • Triccas JA, Pinto R, Britton WJ. Destabilized green fluorescent protein for monitoring transient changes in mycobacterial gene expression. Res Microbiol 2002, 153:379-383. 10.1016/S0923-2508(02)01327-X, 12234013.
    • (2002) Res Microbiol , vol.153 , pp. 379-383
    • Triccas, J.A.1    Pinto, R.2    Britton, W.J.3
  • 35
    • 0032567339 scopus 로고    scopus 로고
    • Generation of destabilized green fluorescent protein as a transcription reporter
    • 10.1074/jbc.273.52.34970, 9857028
    • Li XZX, Fang Y, Jiang X, Duong T, Fan C, Huang CC, Kain SR. Generation of destabilized green fluorescent protein as a transcription reporter. J Biol Chem 1998, 273:34970-34975. 10.1074/jbc.273.52.34970, 9857028.
    • (1998) J Biol Chem , vol.273 , pp. 34970-34975
    • Li, X.Z.X.1    Fang, Y.2    Jiang, X.3    Duong, T.4    Fan, C.5    Huang, C.C.6    Kain, S.R.7
  • 37
    • 82055168964 scopus 로고    scopus 로고
    • Cultivation-independent assessment of bacterial viability
    • Hammes F, Berney M, Egli T. Cultivation-independent assessment of bacterial viability. Adv Biochem Eng Biotechnol 2011, 124:123-150.
    • (2011) Adv Biochem Eng Biotechnol , vol.124 , pp. 123-150
    • Hammes, F.1    Berney, M.2    Egli, T.3
  • 38
    • 0034630406 scopus 로고    scopus 로고
    • Assessment of viability of microorganisms employing fluorescence techniques
    • 10.1016/S0168-1605(00)00163-X, 10791743
    • Breeuwer PAT. Assessment of viability of microorganisms employing fluorescence techniques. Int J Food Microbiol 2000, 55:193-200. 10.1016/S0168-1605(00)00163-X, 10791743.
    • (2000) Int J Food Microbiol , vol.55 , pp. 193-200
    • Breeuwer, P.A.T.1
  • 39
    • 74049138270 scopus 로고    scopus 로고
    • Prospects for the Automation of Analysis and Interpretation of Flow Cytometric Data
    • Davey HM. Prospects for the Automation of Analysis and Interpretation of Flow Cytometric Data. Cytometry A 2010, 77A:3-5.
    • (2010) Cytometry A , vol.77 A , pp. 3-5
    • Davey, H.M.1
  • 40
    • 84872270747 scopus 로고    scopus 로고
    • Computational methods for evaluation of cell-based data assessment-Bioconductor
    • 10.1016/j.copbio.2012.09.003, 23062230
    • LeMeur N. Computational methods for evaluation of cell-based data assessment-Bioconductor. Curr Opin Biotechnol 2013, 24:105-111. 10.1016/j.copbio.2012.09.003, 23062230.
    • (2013) Curr Opin Biotechnol , vol.24 , pp. 105-111
    • LeMeur, N.1
  • 41
    • 85003557302 scopus 로고    scopus 로고
    • BD Biosciences 2013, http://static.bdbiosciences.com/documents/accuri/BD_Accuri_Journal_Guidelines_TechBulletin.pdf.
    • (2013) BD Biosciences
  • 42
    • 34547570134 scopus 로고    scopus 로고
    • Limits of propidium iodide as a cell viability indicator for environmental bacteria
    • Shi L, Günther S, Hübschmann T, Wick LY, Harms H, Müller S. Limits of propidium iodide as a cell viability indicator for environmental bacteria. Cytometry A 2007, 71:592-598.
    • (2007) Cytometry A , vol.71 , pp. 592-598
    • Shi, L.1    Günther, S.2    Hübschmann, T.3    Wick, L.Y.4    Harms, H.5    Müller, S.6
  • 43
    • 0034694211 scopus 로고    scopus 로고
    • Studies related to the scale-up of high-cell-density E. coli fed-batch fermentations using multiparameter flow cytometry : effect of a changing microenvironment with respect to glucose and dissolved oxygen concentration
    • 10.1002/1097-0290(20001120)70:4<381::AID-BIT3>3.0.CO;2-0, 11005920
    • Hewitt CJ, Nebe-Von Caron G, Axelsson B, Mc Farlane CM, Nienow AW. Studies related to the scale-up of high-cell-density E. coli fed-batch fermentations using multiparameter flow cytometry : effect of a changing microenvironment with respect to glucose and dissolved oxygen concentration. Biotechnol Bioeng 2000, 70:381-390. 10.1002/1097-0290(20001120)70:4<381::AID-BIT3>3.0.CO;2-0, 11005920.
    • (2000) Biotechnol Bioeng , vol.70 , pp. 381-390
    • Hewitt, C.J.1    Nebe-Von Caron, G.2    Axelsson, B.3    Mc Farlane, C.M.4    Nienow, A.W.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.