Optimized RNA ISH, RNA FISH and protein-RNA double labeling (IF/FISH) in Drosophila ovaries

Nature Protocols

Volumn 8, Issue 11, 2013, Pages 2158-2179

  Zimmerman, Sandra G ^a   Peters, Nathaniel C ^a   Altaras, Ariel E ^a   Berg, Celeste A ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; ANTIGEN; DIGOXIGENIN ANTIBODY; HUMAN SERUM ALBUMIN; ISOPROPAMIDE IODIDE; PROTEINASE K; REAGENT; RIBONUCLEASE; UNCLASSIFIED DRUG; XYLENE;

ARTICLE; CLINICAL PROTOCOL; DROSOPHILA; ENZYME INACTIVATION; EXPERIMENTAL DESIGN; EXPERIMENTAL STUDY; FLUORESCENCE IN SITU HYBRIDIZATION; GENETIC PROCEDURES; IMAGE PROCESSING; IN SITU HYBRIDIZATION; INCUBATION TEMPERATURE; INCUBATION TIME; INTERMETHOD COMPARISON; MICROSCOPY; NONHUMAN; OVARY; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; PROTEIN DETERMINATION; PROTEIN IMMUNOFLUORESCENCE RNA FLUORESCENCE IN SITU HYBRIDIZATION DOUBLE LABELING; QUALITY CONTROL; REHYDRATION; RNA PROBE; SIGNAL DETECTION; TISSUE FIXATION; TISSUE PREPARATION; TISSUE STRUCTURE;

ANIMALS; DETERGENTS; DROSOPHILA; FEMALE; IN SITU HYBRIDIZATION; IN SITU HYBRIDIZATION, FLUORESCENCE; OVARY; RNA; RNA-BINDING PROTEINS; XYLENES;

EID: 84887217282     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.136     Document Type: Article

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