Quantification of free cysteines in membrane and soluble proteins using a fluorescent dye and thermal unfolding

Nature Protocols

Volumn 8, Issue 11, 2013, Pages 2090-2097

  Branigan, Emma ^a   Pliotas, Christos ^a   Hagelueken, Gregor ^a,b   Naismith, James H ^a  

^a UNIVERSITY OF ST ANDREWS   (United Kingdom)

^b UNIVERSITY OF BONN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

7 DIETHYLAMINO 3 [[4' (IODOACETYL)AMINO]PHENYL] 4 METHYLCOUMARIN; CYSTEINE; DEHYDROALANINE; FLUORESCENT DYE; MEMBRANE PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID ANALYSIS; ARTICLE; CHEMICAL REACTION; CONTROLLED STUDY; FLUORESCENCE; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN STABILITY; PROTEIN UNFOLDING; QUANTITATIVE ANALYSIS; SPIN LABELING; TEMPERATURE; TEMPERATURE RELATED PHENOMENA; THERMAL UNFOLDING;

CYSTEINE; FLUORESCENT DYES; MEMBRANE PROTEINS; PROTEIN STABILITY; PROTEIN UNFOLDING; PROTEINS; SOLUBILITY; SPECTROMETRY, FLUORESCENCE; SULFHYDRYL COMPOUNDS; TEMPERATURE;

EID: 84887006416     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.128     Document Type: Article

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