Steroid extraction: Get the best out of faecal samples

Wiener Tierarztliche Monatsschrift

Volumn 100, Issue 9-10, 2013, Pages 238-246

  Palme, R ^a   Touma, C ^b   Arias, N ^a   Dominchin, M F ^a   Lepschy, M ^a  

^a UNIVERSITY OF VETERINARY MEDICINE   (Austria)

^b MAX PLANCK INSTITUTE OF PSYCHIATRY   (Germany)

Author keywords

Extraction; Faeces; Non invasive hormone monitoring; Reproduction; Review; Stress

Indexed keywords

EID: 84886667230     PISSN: 0043535X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (56)

1. AYRES, K. L., BOOTH, R. K., HEMPELMANN, J. A., KOSKI, K. L., EMMONS, C. K., BAIRD, R. W., BALCOMB-BARTOK, K., HANSON, M. B., FORD, M. J., WASSER, S. K. (2012): Distinguishing the impacts of inadequate prey and vessel traffic on an endangered killer whale (Orcinus orca) population. PLoS One 7, e36842.
2. BAHR, N., PALME, R., MÖHLE, U., HODGES, K., HEISTERMANN, M. (2000): Comparative aspects of the metabolism and excretion of cortisol in three individual nonhuman primates. Gen Comp Endocrinol 117, 427-438.
3. BEEHNER, J. C., WHITTEN, P.L. (2004): Modifications of a field method for fecal steroid analysis in baboons. Physiol Behav 82, 269-277.
4. BROWN, J. L., WASSER, S. K., WILDT, D. E., GRAHAM, L. H. (1994): Comparative aspects of steroid hormone metabolism and ovarian activity in felids, measured noninvasively in feces. Biol Reprod 51, 776-786.
5. DANTZER, B., MCADAM, A. G., PALME, R., HUMPHRIES, M. M., BOUTIN, S., BOONSTRA, R. (2011): Maternal androgens and behavior in free-ranging North American red squirrels. Anim Behav 81, 469-479.
6. DANTZER, B., MCADAM, A. G., PALME, R., HUMPHRIES, M. M., BOUTIN, S., BOONSTRA, R. (2011): How does diet affect fecal steroid hormone metabolite concentrations? An experimental examination in red squirrels. Gen Comp Endocrinol 174, 124-131.
7. DANTZER, B., NEWMAN, A. E. M., BOONSTRA, R., PALME, R., BOUTIN, S., HUMPHRIES, M. M., MCADAM, A. G. (2013): Density triggers maternal hormones that increase adaptive offspring growth in a wild mammal. Science 340, 1215-1217.
8. ELLIS, T., SANDERS, M. B., SCOTT, A. P. (2013): Noninvasive monitoring of steroids in fishes. Wien Tierärztl Monat - Vet Med Austria 100, 255-269.
9. FRYNTA, D., NOVAKOVA, M., KUTALOVA, H., PALME, R., SEDLACEK, F. (2009): Apparatus for collection of fecal samples from undisturbed spiny mice (Acomys cahirinus) living in a complex social group. J Am Ass Lab Anim Sci 48, 196-201.
10. FULLER, G., MARGULIS, S. W., SANTYMIRE, R. (2011): The effectiveness of indigestible markers for identifying individual animal feces and their prevalence of use in North American zoos. Zoo Biol 30, 379-398.
11. GANSWINDT, A., MUILWIJK, C., ENGELKES, M., MÜNSCHER, S., BERTSCHINGER, H., PARIS, M., PALME, R., CAMERON, E. Z., BENNETT, N. C., DALERUM, F. (2012): Validation of non-invasive monitoring of adrenocortical activity in ground feeding Aardwolves (Proteles cristata); Exemplifying the influence of consumption of inorganic material for fecal steroid analysis. Physiol Biochem Zool 85, 194-199.
12. GOYMANN, W. (2005): Non-invasive monitoring of hormones in bird droppings: Physiological validation, sampling, extraction, sex differences, and the influence of diet on hormone metabolite levels. Ann NY Acad Sci 1046, 35-53.
13. GOYMANN, W. (2012): On the use of non-invasive hormone research in uncontrolled, natural environments: The problem with sex, diet, metabolic rate and the individual. Methods Ecol Evol 3, 757-765.
14. HIRSCHENHAUSER, K., KOTRSCHAL, K., MÖSTL, E. (2005): Synthesis of measuring steroid metabolites in goose feces. Ann NY Acad Sci 1046, 138-153.
15. HUBER, S., PALME, R., ARNOLD, W. (2003): Effects of season, sex, and sample collection on concentrations of fecal cortisol metabolites in red deer (Cervus elaphus). Gen Comp Endocrinol 130, 48-54.
16. KALBITZER, U., HEISTERMANN, M. (2013): Long-term storage effects in steroid metabolite extracts from baboon (Papio sp.) faeces - a comparison of three commonly applied storage methods. Meth Ecol Evol 4, 493-500.
17. LEPSCHY, M., TOUMA, C., PALME, R. (2010): Faecal glucocorticoid metabolites: how to express yourself - comparison of absolute amounts versus concentrations in samples from a study in laboratory rats. Lab Anim (UK) 44, 192-198.
18. LEXEN, E., EL-BAHR, S. M., SOMMERFELD-STUR, I., PALME, R., MÖSTL, E. (2008): Monitoring the adrenocortical response to disturbances in sheep by measuring glucocorticoid metabolites in faeces. Wien Tierärztl Monat - Vet Med Austria 95, 64-71.
19. LYNCH, J. W., KHAN, M. Z., ALTMANN, J., NJAHIRA, M. N., RUBENSTEIN, N. (2003): Concentrations of four fecal steroids in wild baboons: short-term storage conditions and consequences for data interpretation. Gen Comp Endocrinol 132, 264-271.
20. MATSUMURO, M., SANKAI, T., CHO, F., YOSHIKAWA, Y., YOSHIDA, T. (1999): A two-step extraction method to measure fecal steroid hormones in female Cynomolgus Monkeys (Macaca fascicularis). Am J Primatol 48, 291-298.
21. MERL, S., SCHERZER, S., PALME, R., MÖSTL, E. (2000): Pain causes increased concentrations of glucocorticoid metabolites in equine faeces. J Equine Vet Sci 20, 586-590.
22. MILLSPAUGH, J. J., WASHBURN, B. E. (2004): Use of fecal glucocorticoid metabolite measures in conservation biology research: considerations for application and interpretation. Gen Comp Endocrinol 138, 189-199.
23. MILLSPAUGH, J. J., WASHBURN, B. E., MILANICK, M. A., SLOTOW, R., VAN DYK, G. (2003): Effects of heat and chemical treatments on fecal glucocorticoid measurements: implications for samples transport. Wildlife Soc Bull 31, 399-406.
24. MONTIGLIO, P. O., PELLETIER, F., PALME, R., GARANT, D., HUMPHRIES, M., REALE, D., BOONSTRA, R. (2012): Non-invasive monitoring of fecal cortisol metabolites in the Eastern chipmunk (Tamias striatus): validation and comparison of two enzyme immunoassays. Physiol Biochem Zool 85, 183-199.
25. MORROW, C. J., KOLVER, E. S., VERKERK, G. A., MATTHEWS, L. R. (2002): Fecal glucocorticoid metabolites as a measure of adrenal activity in dairy cattle. Gen Comp Endocrinol 126, 229-241.
26. MÖSTL, E., NÖBAUER, H., CHOI, H. S., WURM, W., BAMBERG, E. (1983): Trächtigkeitsdiagnose bei der Stute mittels Östrogenbestimmung im Kot. Prakt Tierarzt 64, 491-492.
27. MÖSTL, E., MESSMANN, S., BAGU, E., ROBIA, C., PALME, R. (1999): Measurement of glucocorticoid metabolite concentrations in faeces of domestic livestock. J Vet Med A 46, 621-632.
28. MÖSTL, E., PALME, R. (2002): Hormones as indicators of stress. Dom Anim Endocrinol 23, 67-74.
29. MÖSTL, E., RETTENBACHER, S., PALME, R. (2005): Measurement of corticosterone metabolites in birds' droppings: An analytical approach. Ann NY Acad Sci 1040, 17-34.
30. MURRAY, C.M., HEITZ, M. R., LONSDORF, E. V., PARR, L. A., SANTYMIRE, R. S. (2013): Validation of a field technique and characterization of fecal glucocorticoid metabolite analysis in wild chimpanzees (Pan troglodytes). Am J Primatol 75, 57-64.
31. MURTAGH, R., BEHRINGER, V., DESCHNER, T. (2013): LC-MS as a method for non-invasive measurement of steroid hormones and their metabolites in urine and faeces of animals. Wien Tierärztl Monat - Vet Med Austria 100, 247-254.
32. PALME, R. (2005): Measuring fecal steroids: Guidelines for practical application. Ann NY Acad Sci 1046, 75-80.
33. PALME, R. (2012): Monitoring stress hormone metabolites as a useful, non-invasive tool for welfare assessment in farm animals. Anim Welfare 21, 331-337.
34. PALME, R. (2013): http://www.researcherid.com/rid/A-3499-2008-publiclist: "Non-invasive FCM", regularly updated; last accessed: August 30, 2013.
35. 14C-steroid hormones via faeces and urine in domestic livestock. Anim Reprod Sci 43, 43-63.
36. PALME, R., MÖSTL, E. (1997): Measurement of cortisol metabolites in faeces of sheep as a parameter of cortisol concentration in blood. Zeitschrift Saugetierkunde - Int J Mammal Biol 62(Suppl 2), 192-197.
37. 14C-progesterone in domestic livestock. Reprod Dom Anim 32, 199-206.
38. PALME, R., RETTENBACHER, S., TOUMA, C., EL-BAHR, S. M., MÖSTL, E. (2005): Stress hormones in mammals and birds: Comparative aspects regarding metabolism, excretion and noninvasive measurement in fecal samples. Ann NY Acad Sci 1040, 162-171.
39. PAPPANO, D. J., ROBERTS, E. K., BEEHNER, J. C. (2010): Testing extraction and storage parameters for a fecal hormone method. Am J Primatol 72, 934-941.
40. RETTENBACHER, S., MÖSTL, E., HACKL, R., GHAREEB, K., PALME, R. (2004): Measurement of corticosterone metabolites in chicken droppings. Brit Poult Sci 45, 704-711.
41. SANDERS, H., RAJAMAHENDRAN, R., BURTON, B. (1994): The development of a simple fecal immunoreactive progestin assay to monitor reproductive function in swine. Can Vet J 35, 355-358.
42. SANTYMIRE, R. M., ARMSTRONG, D. M. (2010): Development of a field-friendly technique for fecal steroid extraction and storage using the African wild dog (Lycaon pictus). Zoo Biol 29, 289-302.
43. SCHWARZENBERGER, F., BROWN, J. L. (2013): Hormone monitoring: An important tool for the breeding management of wildlife species. Wien Tierärztl Monat - Vet Med Austria 100, 209-225.
44. SCHATZ, S., PALME, R. (2001): Measurement of faecal cortisol metabolites in cats and dogs: A non-invasive method for evaluating adrenocortical function. Vet Res Commun 25, 271-287.
45. SHERIFF, M. J., DANTZER, B., DELEHANTY, B., PALME, R., BOONSTRA, R. (2011): Measuring stress in wildlife: techniques for quantifying glucocorticoids. Oecologia 166, 869-887.
46. SHUTT, K., SETCHELL, J. M., HEISTERMANN, M. (2012): Non-invasive monitoring of physiological stress in the western lowland gorilla (Gorilla gorilla gorilla): Validation of a fecal glucocorticoid assay and methods for practical application in the field. Gen Comp Endocrinol 179, 167-177.
47. TERIO, K. A., BROWN, J. L., MORELAND, R., MUNSON, L. (2002): Comparison of different drying and storage methods on quantifiable concentrations of fecal steroids in the cheetah. Zoo Biol 21, 215-222.
48. TESKEY-GERSTL, A., STEINECK, T. H., BAMBERG, E., PALME, R. (2000): Excretion of corticosteroids in urine and faeces of hares (Lepus europaeus). J Comp Physiol B 170, 163-168.
49. TOUMA, C., PALME, R. (2005): Measuring fecal glucocorticoid metabolites in mammals and birds: The importance of validation. Ann NY Acad Sci 1046, 54-74.
50. TOUMA, C., SACHSER, N., MÖSTL, E., PALME, R. (2003): Effect of sex and time of day on metabolism and excretion of corticosterone in urine and feces of mice. Gen Comp Endocrinol 130, 267-278.
51. TOUMA, C., PALME, R., SACHSER, N., (2004): Analyzing corticosterone metabolites in fecal samples of mice: A noninvasive technique to monitor stress hormones. Horm Beh 45, 10-22.
52. VASCONCELLOS, A. S., CHELINI, M. O. M., PALME, R., GUIMARAES, M. A. B. V., OLIVEIRA, C. A., ADES, C. (2011): Comparison of two methods for glucocorticoid evaluation in maned wolves (Chrysocyon brachyurus). Pesquisa Vet Brasil 31(Suppl 1), 79-83.
53. VOIGTLÄNDER, T., UNTERBERGER, U., TOUMA, C., PALME, R., POLSTER, B., STROHSCHNEIDER, M., DORNER, S., BUDKA, H. (2006): Prominent corticosteroid disturbance in experimental prion disease. Eur J Neurosci 23, 2723-2730.
54. WASSER, S. K., HUNT, K. E., BROWN, J. L., COOPER, K., CROCKETT, C. M., BECHERT, U., MILLSPAUGH, J. J., LARSON, S., MONFORT, S. L. (2000): A generalized fecal glucocorticoid assay for use in a diverse array of nondomestic mammalian and avian species. Gen Comp Endocrinol 120, 260-275.
55. YOUNG, K. M., WALKER, S. L., LANTHIER, C., WADDELL, W. T., MONFORT, S. L., BROWN, J. L. (2004): Noninvasive monitoring of adrenocortical activity in carnivores by fecal glucocorticoid analyses. Gen Comp Endocrinol 137, 148-165.
56. ZIEGLER, T. E., WITTWER, D. J. (2005): Fecal steroid research in the field and laboratory: improved methods for storage, transport, processing, and analysis. Am J Primatol 67, 159-174.