Phenylalanine to leucine point mutation in oxyanion hole improved catalytic efficiency of Lip12 from Yarrowia lipolytica

Enzyme and Microbial Technology

Volumn 53, Issue 6-7, 2013, Pages 386-390

  Kumari, Arti ^a   Gupta, Rani ^a  

^a UNIVERSITY OF DELHI   (India)

Author keywords

Catalytic efficiency; Differential activation energy; Lipase; Oxyanion hole; Yarrowia lipolytica

Indexed keywords

CATALYTIC EFFICIENCIES; ENZYME-SUBSTRATE COMPLEXES; OXYANION HOLE; SPECIFIC ACTIVITY; SUBSTRATE SPECIFICITY; TRANSITION STATE COMPLEX; TRIACYL GLYCERIDE; YARROWIA LIPOLYTICA;

ACTIVATION ENERGY; AMINO ACIDS; BINDING ENERGY; CATALYST ACTIVITY; CHAIN LENGTH; EFFICIENCY; LIPASES; POSITIVE IONS; STABILIZATION;

SUBSTRATES;

ALLYL ISOTHIOCYANATE; ARACHIS OIL; COCONUT OIL; CORN OIL; COTTON SEED OIL; LEUCINE; NEEM OIL; OLIVE OIL; PALMITIC ACID; PHENYLALANINE; STEARIC ACID; SUNFLOWER OIL; TRIACYLGLYCEROL;

ARTICLE; BINDING KINETICS; CATALYSIS; COMPUTER MODEL; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; HYDROLYSIS; MUTATIONAL ANALYSIS; NONHUMAN; POINT MUTATION; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; WILD TYPE; YARROWIA LIPOLYTICA;

YARROWIA LIPOLYTICA;

CATALYTIC EFFICIENCY; DIFFERENTIAL ACTIVATION ENERGY; LIPASE; OXYANION HOLE; YARROWIA LIPOLYTICA;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CATALYSIS; CATALYTIC DOMAIN; ENZYME STABILITY; FUNGAL PROTEINS; LEUCINE; LIPASE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PHENYLALANINE; PLANT OILS; POINT MUTATION; SUBSTRATE SPECIFICITY; THERMODYNAMICS; YARROWIA;

EID: 84886414467     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2013.08.004     Document Type: Article

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