Improving ethanol tolerance of Saccharomyces cerevisiae by overexpressing an ATP-binding cassette efflux pump

Chemical Engineering Science

Volumn 103, Issue , 2013, Pages 74-78

  Yang, Kyung Mi ^a   Woo, Ji Min ^a   Lee, Sun Mee ^b   Park, Jin Byung ^a  

^a Ewha Womans University   (South Korea)

^b Kyungil University   (South Korea)

Author keywords

ADP1; Ethanol; Ethanol fermentation; Ethanol tolerance; Multidrug efflux pump; Saccharomyces cerevisiae

Indexed keywords

CLONING; YEAST;

ADP1; ATP-BINDING CASSETTE; ENGINEERED STRAINS; ETHANOL FERMENTATION; ETHANOL PRODUCTIVITY; ETHANOL TOLERANCE; MULTIDRUG EFFLUX PUMPS; SPECIFIC GROWTH RATE;

ETHANOL;

EID: 84886101977     PISSN: 00092509     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ces.2012.09.015     Document Type: Article

References (18)

1. Dunlop M.J., Dossani Z.Y., Szmidt H.L., Chu H.C., Lee T.S., Keasling J.D., Hadi M.Z., Mukhopadhyay A. Engineering microbial biofuel tolerance and export using efflux pumps. Mol. Syst. Biol. 2011, 7:487.
2. Isken S., Derks A., Wolffs P.F.G., de Bont J.A.M. Effect of organic solvents on the yield of solvent-tolerant Pseudomonas putida. Appl. Environ. Microbiol. 1999, 65:2631-2635.
3. Jungwirth H., Kuchler K. Yeast ABC transporters-a tale of sex, stress, drugs and aging. FEBS Lett. 2006, 580:1131-1138.
4. Kieboom J., Dennis J.J., de Bont J.A.M., Zylstra G.J. Identification and molecular characterization of an efflux pump involved in Pseudomoans putida S12 solvent tolerance. J. Biol. Chem. 1998, 273:85-91.
5. Ma M.G., Liu Z.L. Mechanisms of ethanol tolerance in Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 2010, 87:829-845.
6. Murakami S. Multidrug recognition and pumping by bacterial multidrug efflux transporter. J. Pharmacol. Sci. 2009, 109:33P. 33P.
7. Nikaido H., Takatsuka Y. Mechanisms of RND multidrug efflux pumps. Biochim. Biophys. Acta-Proteins Proteomics 2009, 1794:769-781.
8. Ramos J.L., Duque E., Callegos M.-T., Codoy P., Ramos-Gonzlez M.I., Rojas A., Teran W., Segura A. Mechanisms of solvent tolerance in gram-negative bacteria. Annu. Rev. Microbiol. 2002, 56:743-768.
9. Rees D.C., Johnson E., Lewinson O. ABC transporters: the power to change. Nat. Rev. Mol. Cell Biol. 2009, 10:218-227.
10. Rojas A., Duque E., Mosqueda G., Golden G., Hurtado A., Ramos J.L., Segura A. Three efflux pumps are required to provide efficient tolerance to toluene in Pseudomonas putida DOT-T1E. J. Bacteriol. 2001, 183:3967-3973.
11. Rosa M.F., Sa-Correia I. Intracellular acidification does not account for inhibition of Saccharomyces cerevisiae growth in the presence of ethanol. FEMS Microbiol. Lett. 1996, 135:271-274.
12. Sadava D., Hamman W. RNA interference against MDR1 but not MRP1 reverses drug resistance in human small-cell lung cancer cells. J. Cancer Mol. 2007, 3:91-94.
13. Salgueiro S.P., Sá-Correia I., Novais J.M. Ethanol induced leakage in Saccharomyces cerevisiae: kinetics and relationship to yeast ethanol tolerance and alcohol fermentation productivity. Appl. Environ. Microbiol. 1988, 54:903-909.
14. Sharom F.J. ABC multidrug transporters: structure, function and role in chemoresistance. Pharmacogenomics 2008, 9:105-127.
15. Teixeira M.C., Raposo L.R., Mira N.P., Lourenco A.B., Sa-Correia I. Genome-wide identification of Saccharomyces cerevisiae genes required for maximal tolerance to ethanol. Appl. Environ. Microbiol. 2009, 75:5761-5772.
16. Yang J., Bae J.Y., Lee Y.M., Kwon H., Moon H.Y., Kang H.A., Yee S.B., Kim W., Choi W. Construction of Saccharomyces cerevisiae strains with enhanced ethanol tolerance by mutagenesis of the TATA-binding protein gene and identification of novel genes associated with ethanol tolerance. Biotechnol. Bioeng. 2011, 108:1776-1787.
17. Yang K.-M., Lee N.-R., Woo J.-M., Choi W., Zimmermann M., Blank L.M., Park J.-B. Ethanol reduces mitochondrial membrane integrity and thereby impacts carbon metabolism of Saccharomyces cerevisiae. FEMS Yeast Res. 2012, 12:675-684.
18. Zhao X.Q., Bai F.W. Mechanisms of yeast stress tolerance and its manipulation for efficient fuel ethanol production. J. Biotechnol. 2009, 144:23-30.