Immunogenicity of EIT chimeric protein expressed in transplastomic tobacco plants towards development of an oral vaccine against Escherichia coli O157:H7

Plant Biotechnology Reports

Volumn 7, Issue 4, 2013, Pages 535-546

  Karimi, Farrokh ^a,d   Mousavi, Amir ^a   Salmanian, Ali Hatef ^a   Alizadeh, Houshang ^b   Rafati, Sima ^c  

^a NATIONAL INSTITUTE OF GENETIC ENGINEERING AND BIOTECHNOLOGY   (Iran)

^b UNIVERSITY OF TEHRAN   (Iran)

^c PASTEUR INSTITUTE OF IRAN   (Iran)

^d UNIVERSITY OF MARAGHEH   (Iran)

Author keywords

EIT three component protein; Enterohemorrhagic Escherichia coli; Immune response; Plastid expression

Indexed keywords

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MUS; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; PROKARYOTA;

EID: 84885961169     PISSN: 18635466     EISSN: 18635474     Source Type: Journal    
DOI: 10.1007/s11816-013-0296-x     Document Type: Article

References (48)

1. Amani J, Mousavi SL, Rafat S, Salmanian AH (2009) In silico analysis of chimeric espA, eae and tir fragment of Escherichia coli O157: H7 for oral immunogenic applications. Theor Biol Med Model 6: 28.
2. Amani J, Salmanian AH, Rafati S, Mousavi SL (2010) Immunogenic properties of chimeric protein from espA, eae and tir genes of Escherichia coli O157: H7. Vaccine 28: 6923-6929.
3. Arlen PA, Singleton M, Adamovicz JJ, Ding Y, Davoodi-Semiromi A, Daniell H (2008) Effective plague vaccination via oral delivery of plant cells expressing F1-V antigens in chloroplasts. Infect Immun 76: 3640-3650.
4. Babiuk S, Asper DJ, Rogan D, Mutwiri GK, Potter AA (2008) Subcutaneous and intranasal immunization with type III secreted proteins can prevent colonization and shedding of Escherichia coli O157: H7 in mice. Microb Pathog 45: 7-11.
5. Chebolu S, Daniell H (2009) Chloroplast-derived vaccine antigens and biopharmaceuticals: expression, folding, assembly and functionality. Curr Microbiol Immunol 332: 33-54.
6. Daniell H (1997) Transformation and foreign gene expression in plants by microprojectile bombardment. Methods Mol Biol 62: 463-489.
7. Daniell H, Lee SB, Panchal T, Wiebe P (2001) Expression of native cholera toxin B sub-unit gene and assembly as functional oligomers in transgenic tobacco chloroplasts. J Mol Biol 311: 1001-1009.
8. Daniell H, Watson J, Koya V, Leppla SH (2004) Expression of Bacillus anthracis protective antigen in transgenic chloroplasts of tobacco, a non-food/feed crop. Vaccine 22: 4374-4384.
9. Daniell H, Ruiz D, Dhingra A (2005) Chloroplast genetic engineering to improve agronomic traits. Methods Mol Biol 286: 111-138.
10. Davoodi-Semiromi A, Samson N, Daniell H (2009) The green vaccine: a global strategy to combat infectious and autoimmune diseases. Hum Vaccine 5(7): 488-493.
11. Dean-Nystrom EA, Gansheroff LJ, Mills M, Moon HW, O'Brien AD (2002) Vaccination of pregnant dams with intimin(O157) protects suckling piglets from Escherichia coli O157: H7 infection. Infect Immun 70: 2414-2418.
12. Dziva F, Vlisidou I, Crepin VF, Wallis TS, Frankel G, Stevens MP (2007) Vaccination of calves with EspA, a key colonization factor of Escherichia coli O157: H7, induces antigen-specific humoral responses but does not confer protection against intestinal colonization. Vet Microbiol 123: 254-261.
13. Fischer R, Stoger E, Schillberg S, Christou P, Twyman RM (2004) Plant based production of biopharmaceuticals. Curr Opin Plant Biol 7: 152-158.
14. Frankel G, Phillips AD, Rosenshine I, Dougan G, Kaper JB, Knutton S (1998) Enteropathogenic and enterohaemorrhagic Escherichia coli: more subversive elements. Mol Microbiol 30: 911-921.
15. Giddings G, Allison G, Brooks D, Carter A (2000) Transgenic plants as factories for biopharmaceuticals. Nat Biotechnol 18: 1151-1155.
16. Grauke LJ, Kudva IT, Yoon JW, Hunt CW, Williams CJ, Hovde CJ (2002) Gastrointestinal tract location of Escherichia coli O157: H7 in ruminants. Appl Environ Microbiol 68: 2269-2277.
17. Judge NA, Mason HS, O'Brien AD (2004) Plant cell-based intimin vaccine given orally to mice primed with intimin reduces time of Escherichia coli O157: H7 shedding in feces. Infect Immun 72: 168-175.
18. Kudva IT, Jelacic S, Tarr PI, Youderian P, Hovde CJ (1999) Biocontrol of O157 with O157-specific bacteriophages. Appl Environ Microbiol 65: 3767-3773.
19. Kuhne SA, Hawes WS, La Ragione RM, Woodwaed MJ, Whitelam GC, Gough KC (2004) Isolation of recombinant antibodies against EspA and intimin of Escherichia coli 0157: H7. J Clin Microbiol 42: 2966-2976.
20. Lee SB, Kwon HB, Kwon SJ, Park SC, Jeong MJ, Han SE, Byun MO, Daniell H (2003) Accumulation of trehalose within transgenic chloroplasts confers drought tolerance. Mol Breed 11: 1-13.
21. Lim JY, Sheng H, Keun SS, Park YH, Hovde CJ (2007) Characterization of an Escherichia coli O157: H7 plasmid O157 deletion mutant and its survival and persistence in cattle. Appl Environ Microbiol 73: 2037-2047.
22. Limaye A, Koya V, Samsam M, Daniell H (2006) Receptor-mediated oral delivery of a bioencapsulated green fluorescent protein expressed in transgenic chloroplasts into the mouse circulatory system. FASEB J 20: 959-961.
23. Ma JK, Chikwamba R, Sparrow P, Fischer R, Mahoney R, Twyman RM (2005) Plant-derived pharmaceuticals-the road forward. Trends Plant Sci 10: 580-585.
24. Madisis P, Osathanunkul M, Georgopoulou U, Gisby MF, Mudd EA, Nianiou I, Tsitoura P, Mavromara P, Tsaftaris A, day A (2010) A hepatitis C virus core polypeptide expressed in chloroplasts detects anti-core antibodies in infected human sera. J Biotechnol 145: 377-386.
25. Maliga P (2002) Engineering the plastid genome of higher plants. Curr Opin Plant Biol 5: 164-172.
26. Maliga P (2003) Progress towards commercialization of plastid transformation technology. Trends Biotechnol 21: 20-28.
27. Maliga P (2004) Plastid transformation in higher plants. Annu Rev Plant Biol 55: 289-313.
28. McNeilly TN, Mitchell MC, Rosser T, McAteer S, Low JC, Smith DGE, Huntley JF, Mahajan A, Gally DL (2010) Immunization of cattle with a combination of purified intimin-531, EspA and Tir significantly reduces shedding of Escherichia coli O157: H7 following oral challenge. Vaccine 28: 1422-1428.
29. Molina A, Hervas-Stubbs S, Daniell H, Mingo-Castel AM, Veramendi J (2004) High yield expression of a viral peptide animal vaccine in transgenic tobacco chloroplasts. Plant Biotechnol 2: 141-153.
30. Moxley RA (2004) Escherichia coli 0157: H7: an update on intestinal colonization and virulence mechanisms. Anim Health Res Rev 511: 15-33.
31. Mundy R, Schuller S, Girard F, Fairbrother JM, Phillips AD, Frankel G (2007) Functional studies of intimin in vivo and ex vivo: implications for host specificity and tissue tropism. Microbiology 153: 959-967.
32. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-479.
33. Nataro JP, Kaper JB (1998) Diarrheagenic Escherichia coli. Clin Microbiol Rev 11: 142-201.
34. Potter AA, Klashinsky S, Li Y, Frey E, Townsend H, Rogan D, Erickson G, Hinkley S, Klopfenstein T, Moxley RA, Smith DR, Finlay BB (2004) Decreased shedding of Escherichia coli O157: H7 by cattle following vaccination with type III secreted proteins. Vaccine 22: 362-369.
35. Reddy VS, Leellavathi S (2003) Chloroplast expression of His-tagged GUS-fusions: a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors. Mol Breed 11: 49-58.
36. Reddy VS, Leellavathi S, Selvapandiyan A, Raman R, Giovanni F, Shhukla V, Bhatnagar RR (2002) Analysis of chloroplast transformed tobacco plants with cry1Ia5 under rice psbA transcriptional elements reveal high level expression of Bt toxin without imposing yield penalty and stable inheritance of transplastome. Mol Breed 9: 259-269.
37. Ruiz ON, Daniell H (2005) Engineering cytoplasmic male sterility via the chloroplast genome by the expression of β-ketothiolase. Plant Physiol 138: 1232-1246.
38. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor.
39. Schlundt J, Toyofuku H, Jansen J, Herbst SA (2004) Emerging food-borne zoonoses. Rev Sci Tech 23: 513-533.
40. Scotti N, Alagna F, Ferraiolo E, Formisano G, Sannino L, Buonaguro L, Stradis AD, Vitale A, Monti L, Grillo S, Buonaguro FM, Cardi T (2009) High-level expression of the HIV-1 Pr55gg polyprotein in transgenic tobacco chloroplast. Planta 229: 1109-1122.
41. Shao HB, He DM, Qian KX, Shen GF, Su ZL (2008) The expression of classical swine fever virus structural protein E2 gene in tobacco chloroplasts for applying chloroplasts as bioreactors. CR Biol 331: 179-184.
42. Shen H, Qian B, Chen W, Liu Z, Yang L, Zhang D, Liang W (2010) Immunogenicity of recombinant F4 (K88) fimbrial adhesin FaeG expressed in tobacco chloroplast. Acta Biochim Biophys Sin 42: 558-567.
43. Soria-Guerra RE, Alpuche-Solis AG, Rosales-Mendoza S, Moreno-Fierros L, Bendik EM, Martinez-Gonzales L, Korban SS (2009) Expression of a multi-epitope DPT fusion protein in transplastomic tobacco plants retains both antigenicity and immunogenicity of all three components of the functional oligomer. Planta 229: 1293-1302.
44. Stevens MP, Diemen PM, Dziva F, Jones PW, Wallis TS (2002) Options for the control of enterohaemorrhagic Escherichia coli in ruminants. Microbiology 148: 3767-3778.
45. Tkalcic S, Zhao T, Harmon BG, Doyle MP, Brown CA, Zhao P (2003) Fecal shedding of enterohemorrhagic Escherichia coli I weaned calves following treatment with probiotic Escherichia coli. J Food Prot 66: 1184-1189.
46. Tregoning JS, Nixon P, Kuroda H, Svab Z, Clare S, Bowe F (2003) Expression of tetanus toxin fragment C in tobacco chloroplast. Nucleic Acid Res 31(4): 1174-1179.
47. Yoon JW, Hovde CJ (2008) All blood, no stool: enterohemorrhagic Escherichia coli O157: H7 infection. J Vet Sci 9: 219-231.
48. Yu J, Kaper JB (1992) Cloning and characterization of the eae gene of enterohaemorrhagic Escherichia coli O157: H7. Mol Microbiol 6: 411-417.