High-level expression of the HIV-1 Pr55gag polyprotein in transgenic tobacco chloroplasts

Planta

Volumn 229, Issue 5, 2009, Pages 1109-1122

  Scotti, Nunzia ^a   Alagna, Fiammetta ^a   Ferraiolo, Enrico ^a   Formisano, Gelsomina ^a   Sannino, Lorenza ^a   Buonaguro, Luigi ^b   De Stradis, Angelo ^c   Vitale, Alessandro ^d   Monti, Luigi ^a   Grillo, Stefania ^a   Buonaguro, Franco M ^b   Cardi, Teodoro ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^b NATIONAL CANCER INSTITUTE   (Italy)

^c CNR   (Italy)

^d CNR Consiglio Nazionale delle Ricerche   (Italy)

Author keywords

HIV 1 Pr55gag; Plant factories; Plastid transformation; Protein accumulation; Tobacco

Indexed keywords

AGROBACTERIUM; ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; NICOTIANA TABACUM; RHIZOBIUM;

EID: 63349105635     PISSN: 00320935     EISSN: 14322048     Source Type: Journal    
DOI: 10.1007/s00425-009-0898-2     Document Type: Article

References (57)

1. J Bally E Paget M Droux C Job M Dubald 2008 Both the stroma and thylakoid lumen of tobacco chloroplasts are competent for the formation of disulphide bonds in recombinant proteins Plant Biotechnol J 6 46 61
2. A Barbante S Irons C Hawes L Frigerio A Vitale E Pedrazzini 2008 Anchorage to the cytosolic face of the endoplasmic reticulum membrane: a new strategy to stabilize a cytosolic recombinant antigen in plants Plant Biotechnol J 6 560 575
3. I Birch-Machin CA Newell JM Hibberd JC Gray 2004 Accumulation of rotavirus VP6 protein in chloroplasts of transplastomic tobacco is limited by protein stability Plant Biotechnol J 2 261 270
4. L Buhot E Horvàth P Medgyesy S Lerbs-Mache 2006 Hybrid transcription system for controlled plastid transgene expression Plant J 46 700 707
5. gag virus-like particles expressing multiple HIV-1 epitopes, including a gp120 protein derived from an Ugandan HIV-1 isolate of subtype A Antiviral Res 49 35 47
6. L Buonaguro C Devito ML Tornesello U Schröder B Wahren J Hinkula FM Buonaguro 2007 DNA-VLP prime-boost intra-nasal immunization induces cellular and humoral anti-HIV-1 systemic and mucosal immunity with cross-clade neutralizing activity Vaccine 25 5968 5977
7. T Cardi T Bastia L Monti ED Earle 1999 Organelle DNA and male fertility variation in Solanum spp. and interspecific somatic hybrids Theor Appl Genet 99 819 828
8. H Daniell 2002 Molecular strategies for gene containment in transgenic crops Nat Biotechnol 20 581 586
9. H Daniell SJ Streatfield K Wycoff 2001 Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants Trends Plant Sci 6 219 226
10. M de Virgilio F De Marchis M Bellucci D Mainieri M Rossi E Benvenuto S Arcioni A Vitale 2008 The human immunodeficiency virus antigen Nef forms protein bodies in leaves of transgenic tobacco when fused to zeolin J Exp Bot 59 2815 2829
11. L Deml C Speth MP Dierich H Wolf R Wagner 2005 Recombinant HIV-1 Pr55gag virus-like particles: potent stimulators of innate and acquired immune responses Mol Immunol 42 259 277
12. A Fernández-San Millan SM Ortigosa S Hervas-Stubbs P Corral-Martinez JM Segui-Simarro J Gaetan P Coursaget J Veramendi 2008 Human papillomavirus L1 protein expressed in tobacco chloroplasts self-assembles into virus-like particles that are highly immunogenic Plant Biotechnol J 6 427 441
13. EO Freed 1998 HIV-1 Gag proteins: diverse functions in the virus life cycle Virology 251 1 15
14. R Greco M Michel D Guetard M Cervantes-Gonzalez N Pelucchi S Wain-Hobson F Sala M Sala 2007 Production of recombinant HIV-1/HBV virus-like particles in Nicotiana tabacum and Arabidopsis thaliana plants for a bivalent plant-based vaccine Vaccine 25 8228 8240
15. JC Griffiths SJ Harris GT Layton EL Berrie TJ French NR Burns SE Adams AJ Kingsman 1993 Hybrid human immunodeficiency virus Gag particles as an antigen carrier system: induction of cytotoxic T-cell and humoral responses by a Gag:V3 fusion J Virol 67 3191 3198
16. A Hennig K Bonfig T Roitsch H Warzecha 2007 Expression of the recombinant bacterial outer surface protein A in tobacco chloroplasts leads to thylakoid localization and loss of photosynthesis FEBS J 274 5749 5758
17. S Herz M Füssl S Steiger H-U Koop 2005 Development of novel types of plastid transformation vectors and evaluation of factors controlling expression Transgenic Res 14 969 982
18. AV Karasev S Foulke C Wellens A Rich KJ Shon I Zwierzynski D Hone H Koprowski M Reitz 2005 Plant based HIV-1 vaccine candidate: Tat protein produced in spinach Vaccine 23 1875 1880
19. RH Köhler WR Zipfel WW Webb MR Hanson 1997 The green fluorescent protein as a marker to visualize plant mitochondria in vivo Plant J 11 613 621
20. H Kuroda P Maliga 2001 Complementarity of the 16S rRNA penultimate stem with sequences downstream of the AUG destabilizes the plastid mRNAs Nucleic Acids Res 29 970 975
21. H Kuroda P Maliga 2002 Overexpression of the clpP 5-untranslated region in a chimeric context causes a mutant phenotype, suggesting competition for a clpP-specific RNA maturation factor in tobacco chloroplasts Plant Physiol 129 1600 1606
22. P Lenzi N Scotti F Alagna M Tornesello A Pompa A Vitale A De Stradis L Monti S Grillo F Buonaguro P Maliga T Cardi 2008 Translational fusion of chloroplast-expressed human papilloma virus type 16 L1 capsid protein enhances antigen accumulation in transplastomic tobacco Transgenic Res 17 1091 1102
23. A Lössl K Bohmert H Harloff C Eibl S Muhlbauer H-U Koop 2005 Inducible trans-activation of plastid transgenes: expression of the R. eutropha phb operon in transplastomic tobacco Plant Cell Physiol 46 1462 1471
24. JK-C Ma PMW Drake P Christou 2003 The production of recombinant pharmaceutical proteins in plants Nat Rev Genet 4 794 805
25. P Maliga 2002 Engineering the plastid genome of higher plants Curr Opin Plant Biol 5 164 172
26. C Marusic J Nuttall G Buriani C Lico R Lombardi S Baschieri E Benvenuto L Frigerio 2007 Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells BMC Biotechnol 7 12
27. AK Mattoo M Edelman 1987 Intramembrane translocation and post translational palmitoylation of the chloroplast 32-kDa herbicide-binding protein Proc Natl Acad Sci USA 84 1497 1501
28. MS McCabe M Klaas N Gonzalez-Rabade M Poage JA Badillo-Corona F Zhou D Karcher R Bock JC Gray PJ Dix 2008 Plastid transformation of high-biomass tobacco variety Maryland Mammoth for production of human immunodeficiency virus type 1 (HIV-1) p24 antigen Plant Biotechnol J 6 914 929
29. A Meyers E Chakauya E Shephard FL Tanzer J Maclean A Lynch AL Williamson EP Rybicki 2008 Expression of HIV-1 antigens in plants as potential subunit vaccines BMC Biotechnol 8 53
30. RG Milne E Luisoni 1977 Rapid immune electron microscopy of virus preparations Meth Virol 6 265 281
31. A Molina S Hervas-Stubbs H Daniell AM Mingo-Castel J Veramendi 2004 High-yield expression of a viral peptide animal vaccine in transgenic tobacco chloroplasts Plant Biotechnol J 2 141 153
32. Y Morikawa 2003 HIV capsid assembly Curr HIV Res 1 1 14
33. Y Morikawa T Goto F Momose 2004 Human immunodeficiency virus type 1 Gag assembly through assembly intermediates J Biol Chem 279 31964 31972
34. SK Mühlbauer H-U Koop 2005 External control of transgene expression in tobacco plastids using the bacterial lac repressor Plant J 43 941 946
35. DJ Murphy 2007 Improving containment strategies in biopharming Plant Biotechnol J 5 555 569
36. Nermut NV (1982) Advanced methods in electron microscopy of viruses. In: Howard CR (ed) New developments in practical virology. Alan Liss, New York, pp 1-58
37. P Obregon D Chargelegue PMW Drake A Prada J Nuttall L Frigerio JK-C Ma 2006 HIV-1 p24-immunoglobulin fusion molecule: a new strategy for plant-based protein production Plant Biotechnol J 4 195 207
38. Oey M, Lohse M, Kreikemeyer B, Bock R (2008) Exhaustion of the chloroplast protein synthesis capacity by massive expression of a highly stable protein antibiotic. Plant J. doi: 10.1111/j.1365-313X.2008.03702.x
39. NS Outchkourov J Peters J de Jong W Rademakers MA Jongsma 2003 The promoter-terminator of chrysanthemum rbcS1 directs very high expression levels in plants Planta 216 1003 1012
40. NS Outchkourov B Rogelj B Strukelj MA Jongsma 2003 Expression of sea anemone equistatin in potato. Effects of plant proteases on heterologous protein production Plant Physiol 133 379 390
41. I Parmryd CA Shipton E Swiezewska G Dallner B Andersson 1997 Chloroplastic prenylated proteins FEBS Lett 414 527 531
42. E Pedrazzini G Giovinazzo R Bollini A Ceriotti A Vitale 1994 Binding of BiP to an assembly-defective protein in plant cells Plant J 5 103 110
43. DM Pérez-Filgueira BP Brayfield S Phiri MV Borca C Wood TJ Morris 2004 Preserved antigenicity of HIV-1 p24 produced and purified in high yields from plants inoculated with a tobacco mosaic virus (TMV)-derived vector J Virol Methods 121 201 208
44. N Scotti L Monti T Cardi 2003 Organelle DNA variation in parental Solanum spp. genotypes and nuclear-cytoplasmic interactions in Solanum tuberosum (+) S. commersonii somatic hybrid-backcross progeny Theor Appl Genet 108 87 94
45. S Shchelkunov R Salyaev S Pozdnyakov N Rekoslavskaya A Nesterov T Ryzhova V Sumtsova N Pakova U Mishutina T Kopytina R Hammond 2006 Immunogenicity of a novel, bivalent, plant-based oral vaccine against hepatitis B and human immunodeficiency viruses Biotechnol Lett 28 959 967
46. P Spearman JJ Wang N Vander Heyden L Ratner 1994 Identification of human immunodeficiency virus type 1 Gag protein domains essential to membrane binding and particle assembly J Virol 68 3232 3242
47. SJ Streatfield 2007 Approaches to achieve high-level heterologous protein production in plants Plant Biotechnol J 5 2 15
48. JS Tregoning P Nixon H Kuroda Z Svab S Clare F Bowe N Fairweather J Ytterberg KJ van Wijk G Dougan P Maliga 2003 Expression of tetanus toxin fragment C in tobacco chloroplasts Nucleic Acids Res 31 1174 1179
49. RJ Tritch Y-SE Cheng FH Yin S Erickson-Viitanen 1991 Mutagenesis of protease cleavage sites in the human immunodeficiency virus type 1 Gag polyprotein J Virol 65 922 930
50. RM Twyman E Stoger S Schillberg P Christou R Fischer 2003 Molecular farming in plants: host systems and expression technology Trends Biotechnol 21 570 578
51. O Voinnet S Rivas P Mestre D Baulcombe 2003 An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus Plant J 33 949 956
52. BD Walker DR Burton 2008 Toward an AIDS vaccine Science 320 760 764
53. KR Young SP McBurney LU Karkhanis TM Ross 2006 Virus-like particles: designing an effective AIDS vaccine Methods 40 98 117
54. G Zhang C Leung L Murdin B Rovinski KA White 2000 In planta expression of HIV-1 p24 protein using an RNA plant virus-based expression vector Mol Biotechnol 14 99 107
55. GG Zhang L Rodrigues R Rovinski KA White 2002 Production of HIV-1 p24 protein in transgenic tobacco plants Mol Biotechnol 20 131 136
56. F Zhou JA Badillo-Corona D Karcher N Gonzalez-Rabade K Piepenburg AM Borchers AP Maloney TA Kavanagh JC Gray R Bock 2008 High-level expression of human immunodeficiency virus antigens from the tobacco and tomato plastid genomes Plant Biotechnol J 6 897 913
57. OL Zoubenko LA Allison Z Svab P Maliga 1994 Efficient targeting of foreign genes into the tobacco plastid genome Nucleic Acids Res 22 3819 3824