Crystallization of G Protein-Coupled Receptors

Methods in Cell Biology

Volumn 117, Issue , 2013, Pages 451-468

  Salom, David ^a   Padayatti, Pius S ^a   Palczewski, Krzysztof ^a,b  

^a Polgenix Inc   (United States)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Ammonium sulfate; Crystallization; G protein coupled receptor; Lipidic cubic phase; Purification; Rhodopsin; 2 Adrenergic receptor

Indexed keywords

AMMONIUM SULFATE; BETA 2 ADRENERGIC RECEPTOR; CARAZOLOL; GLUCOSIDE; HYBRID PROTEIN; LYSOZYME; PROPANOLAMINE DERIVATIVE; RHODOPSIN; VIRUS PROTEIN; ZINC ACETATE;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; CATTLE; CHEMISTRY; CRYSTALLIZATION; DENSITY GRADIENT CENTRIFUGATION; G PROTEIN-COUPLED RECEPTOR; GEL CHROMATOGRAPHY; GENETICS; ISOLATION AND PURIFICATION; LIPIDIC CUBIC PHASE; PROTEIN MULTIMERIZATION; PROTEIN SECONDARY STRUCTURE; PURIFICATION; RETINA ROD OUTER SEGMENT; SF9 CELL LINE; SPODOPTERA; X RAY CRYSTALLOGRAPHY; Β2-ADRENERGIC RECEPTOR;

AMMONIUM SULFATE; CRYSTALLIZATION; G PROTEIN-COUPLED RECEPTOR; LIPIDIC CUBIC PHASE; PURIFICATION; RHODOPSIN; Β2-ADRENERGIC RECEPTOR;

AMMONIUM SULFATE; ANIMALS; CATTLE; CENTRIFUGATION, DENSITY GRADIENT; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; GLUCOSIDES; MURAMIDASE; PROPANOLAMINES; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, ADRENERGIC, BETA-2; RECOMBINANT FUSION PROTEINS; RHODOPSIN; ROD CELL OUTER SEGMENT; SF9 CELLS; SPODOPTERA; VIRAL PROTEINS; ZINC ACETATE;

EID: 84885935715     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-408143-7.00024-4     Document Type: Chapter

References (35)

1. Aherne M., Lyons J.A., Caffrey M. A fast, simple and robust protocol for growing crystals in the lipidic cubic phase. Journal of Applied Crystallography 2012, 45(Pt 6):1330-1333.
2. Alexandrov A.I., Mileni M., Chien E.Y., Hanson M.A., Stevens R.C. Microscale fluorescent thermal stability assay for membrane proteins. Structure 2008, 16(3):351-359.
3. Caffrey M., Cherezov V. Crystallizing membrane proteins using lipidic mesophases. Nature Protocols 2009, 4(5):706-731.
4. Caffrey M., Li D., Dukkipati A. Membrane protein structure determination using crystallography and lipidic mesophases: Recent advances and successes. Biochemistry 2012, 51(32):6266-6288.
5. Cherezov V., Caffrey M. Membrane protein crystallization in lipidic mesophases. A mechanism study using X-ray microdiffraction. Faraday Discussions 2007, 136:195-212. discussion 213-129.
6. Cherezov V., Liu J., Griffith M., Hanson M.A., Stevens R.C. LCP-FRAP assay for pre-screening membrane proteins for in meso crystallization. Crystal Growth & Design 2008, 8(12):4307-4315.
7. Cherezov V., Rosenbaum D.M., Hanson M.A., Rasmussen S.G., Thian F.S., Kobilka T.S., et al. High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor. Science 2007, 318(5854):1258-1265.
8. Chun E., Thompson A.A., Liu W., Roth C.B., Griffith M.T., Katritch V., et al. Fusion partner toolchest for the stabilization and crystallization of g protein-coupled receptors. Structure 2012, 20(6):967-976.
9. Dilanian R.A., Streltsov V.A., Quiney H.M., Nugent K.A. Continuous X-ray diffractive field in protein nanocrystallography. Acta Crystallographica. Section A 2013, 69(Pt 1):108-118.
10. Fotiadis D., Liang Y., Filipek S., Saperstein D.A., Engel A., Palczewski K. Atomic-force microscopy: Rhodopsin dimers in native disc membranes. Nature 2003, 421(6919):127-128.
11. Fotiadis D., Liang Y., Filipek S., Saperstein D.A., Engel A., Palczewski K. The G protein-coupled receptor rhodopsin in the native membrane. FEBS Letters 2004, 564(3):281-288.
12. Huang J., Chen S., Zhang J.J., Huang X.Y. Crystal structure of oligomeric β1-adrenergic G protein-coupled receptors in ligand-free basal state. Nature Structural and Molecular Biology 2013, 20(4):419-425.
13. Judge R.A., Swift K., Gonzalez C. An ultraviolet fluorescence-based method for identifying and distinguishing protein crystals. Acta Crystallographica, Section D: Biological Crystallography 2005, 61(Pt 1):60-66.
14. Kang H.J., Lee C., Drew D. Breaking the barriers in membrane protein crystallography. International Journal of Biochemistry & Cell Biology 2013, 45(3):636-644.
15. Kissick D.J., Gualtieri E.J., Simpson G.J., Cherezov V. Nonlinear optical imaging of integral membrane protein crystals in lipidic mesophases. Analytical Chemistry 2010, 82(2):491-497.
16. Landau E.M., Rosenbusch J.P. Lipidic cubic phases: A novel concept for the crystallization of membrane proteins. Proceedings of the National Academy of Sciences of the United States of America 1996, 93(25):14532-14535.
17. Lodowski D.T., Salom D., Le Trong I., Teller D.C., Ballesteros J.A., Palczewski K., et al. Crystal packing analysis of Rhodopsin crystals. Journal of Structural Biology 2007, 158(3):455-462.
18. Madden J.T., Dewalt E.L., Simpson G.J. Two-photon excited UV fluorescence for protein crystal detection. Acta Crystallographica, Section D: Biological Crystallography 2011, 67(Pt 10):839-846.
19. Manglik A., Kruse A.C., Kobilka T.S., Thian F.S., Mathiesen J.M., Sunahara R.K., et al. Crystal structure of the μ-opioid receptor bound to a morphinan antagonist. Nature 2012, 485(7398):321-326.
20. Milligan G. A day in the life of a G protein-coupled receptor: The contribution to function of G protein-coupled receptor dimerization. British Journal of Pharmacology 2008, 153(Suppl. 1):S216-S229.
21. Okada T., Le Trong I., Fox B.A., Behnke C.A., Stenkamp R.E., Palczewski K. X-ray diffraction analysis of three-dimensional crystals of bovine rhodopsin obtained from mixed micelles. Journal of Structural Biology 2000, 130(1):73-80.
22. Okada T., Takeda K., Kouyama T. Highly selective separation of rhodopsin from bovine rod outer segment membranes using combination of divalent cation and alkyl(thio)glucoside. Photochemistry and Photobiology 1998, 67(5):495-499.
23. Padayatti P., Palczewska G., Sun W., Palczewski K., Salom D. Imaging of protein crystals with two-photon microscopy. Biochemistry 2012, 51(8):1625-1637.
24. Palczewski K., Kumasaka T., Hori T., Behnke C.A., Motoshima H., Fox B.A., et al. Crystal structure of rhodopsin: A G protein-coupled receptor. Science 2000, 289(5480):739-745.
25. Papermaster D.S. Preparation of retinal rod outer segments. Methods in Enzymology 1982, 81:48-52.
26. Ruprecht J.J., Mielke T., Vogel R., Villa C., Schertler G.F. Electron crystallography reveals the structure of metarhodopsin I. EMBO Journal 2004, 23(18):3609-3620.
27. Salom D., Le Trong I., Pohl E., Ballesteros J.A., Stenkamp R.E., Palczewski K., et al. Improvements in G protein-coupled receptor purification yield light stable rhodopsin crystals. Journal of Structural Biology 2006, 156(3):497-504.
28. Salom D., Li N., Zhu L., Sokal I., Palczewski K. Purification of the G protein-coupled receptor rhodopsin for structural studies. G protein-coupled receptor-protein interactions 2005, 1-17. John Wiley & Sons, Inc, Hoboken. S.R. George, B.F. O'Dowd (Eds.).
29. Salom D., Lodowski D.T., Stenkamp R.E., Le Trong I., Golczak M., Jastrzebska B., et al. Crystal structure of a photoactivated deprotonated intermediate of rhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006, 103(44):16123-16128.
30. Spalink J.D., Reynolds A.H., Rentzepis P.M., Sperling W., Applebury M.L. Bathorhodopsin intermediates from 11-cis-rhodopsin and 9-cis-rhodopsin. Proceedings of the National Academy of Sciences of the United States of America 1983, 80(7):1887-1891.
31. Stenkamp R.E. Alternative models for two crystal structures of bovine rhodopsin. Acta Crystallographica, Section D: Biological Crystallography 2008, D64(Pt 8):902-904.
32. Suda K., Filipek S., Palczewski K., Engel A., Fotiadis D. The supramolecular structure of the GPCR rhodopsin in solution and native disc membranes. Molecular Membrane Biology 2004, 21(6):435-446.
33. Wu B., Chien E.Y., Mol C.D., Fenalti G., Liu W., Katritch V., et al. Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists. Science 2010, 330(6007):1066-1071.
34. Wu H., Wacker D., Mileni M., Katritch V., Han G.W., Vardy E., et al. Structure of the human kappa-opioid receptor in complex with JDTic. Nature 2012, 485(7398):327-332.
35. Xu F., Liu W., Hanson M.A., Stevens R.C., Cherezov V. Development of an automated high throughput LCP-FRAP assay to guide membrane protein crystallization in lipid mesophases. Crystal Growth & Design 2011, 11(4):1193-1201.