Mutant firefly luciferases with improved specific activity and dATP discrimination constructed by yeast cell surface engineering

Applied Microbiology and Biotechnology

Volumn 97, Issue 9, 2013, Pages 4003-4011

  Fushimi, Tatsuya ^a   Miura, Natsuko ^a   Shintani, Hideya ^a   Tsunoda, Hiroyuki ^b   Kuroda, Kouichi ^a   Ueda, Mitsuyoshi ^a  

^a KYOTO UNIVERSITY   (Japan)

^b Central Research Laboratory   (Japan)

Author keywords

dATP discrimination; Firefly luciferase; Molecular display; Protein library; Specific activity; Yeast cell surface engineering

Indexed keywords

AMINO ACIDS; BIOLUMINESCENCE; CELL MEMBRANES; DNA SEQUENCES; ENZYME ACTIVITY; GENE ENCODING; PLANTS (BOTANY); YEAST;

DATP DISCRIMINATION; FIREFLY LUCIFERASE; PROTEIN LIBRARY; SPECIFIC ACTIVITY; YEAST CELL SURFACE;

CELL ENGINEERING;

DEOXYADENOSINE TRIPHOSPHATE; FIREFLY LUCIFERASE; 2'-DEOXYADENOSINE TRIPHOSPHATE; DEOXYADENOSINE PHOSPHATE;

BIOENGINEERING; CYTOLOGY; ENZYME ACTIVITY; MOLECULAR ANALYSIS; MUTATION; YEAST;

ARTICLE; CELL ENGINEERING; CELL LABELING; CELL SURFACE DISPLAY; CELL SUSPENSION; CONTROLLED STUDY; ENZYME SPECIFICITY; ESCHERICHIA COLI; MUTAGENESIS; NONHUMAN; PHOTINUS PYRALIS; PYROSEQUENCING; SACCHAROMYCES CEREVISIAE; SCREENING TEST; SEQUENCE ALIGNMENT; YEAST CELL; GENETICS; METABOLISM; METHODOLOGY; PROTEIN ENGINEERING; YEAST;

PHOTINUS PYRALIS;

DEOXYADENINE NUCLEOTIDES; LUCIFERASES, FIREFLY; PROTEIN ENGINEERING; SUBSTRATE SPECIFICITY; YEASTS;

EID: 84885925211     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4467-4     Document Type: Article

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