Demonstration of catalytic proton acceptor of chitosanase from Paenibacillus fukuinensis by comprehensive analysis of mutant library

Applied Microbiology and Biotechnology

Volumn 85, Issue 1, 2009, Pages 95-104

  Isogawa, Danya ^a   Fukuda, Takeshi ^b   Kuroda, Kouichi ^a   Kusaoke, Hideo ^c   Kimoto, Hisashi ^d   Suye, Shin Ichiro ^b   Ueda, Mitsuyoshi ^a  

^a KYOTO UNIVERSITY   (Japan)

^b UNIVERSITY OF FUKUI   (Japan)

^c FUKUI UNIVERSITY OF TECHNOLOGY   (Japan)

^d FUKUI PREFECTURAL UNIVERSITY   (Japan)

Author keywords

Chitosanase; Family 8 glycoside hydrolase; Paenibacillus fukuinensis; Proton acceptor; Yeast cell surface engineering

Indexed keywords

ACTIVITY ASSAYS; AMINO ACID RESIDUES; CHITOSANASE; COMPREHENSIVE ANALYSIS; ESSENTIAL AMINO ACIDS; GLUCANASE; GLYCOSIDE HYDROLASES; MUTANT LIBRARIES; MUTUAL INTERACTION; PROTON ACCEPTORS; YEAST CELL; YEAST CELL SURFACE;

AMINO ACIDS; CELL MEMBRANES; ENZYME ACTIVITY; ORGANIC ACIDS; PROTONS; SUGARS;

YEAST;

1,4 BETA GLUCANASE; ASPARAGINE; BETA GLUCAN HYDROLASE; CELLULOSE; CHITOSAN; CHITOSANASE; GLUTAMIC ACID; MUTANT PROTEIN; UNCLASSIFIED DRUG; 1,4 GLUCAN; 1,4-GLUCAN; BACTERIAL PROTEIN; GLUCAN; GLUTAMINE; GLYCOSIDASE; PROTON;

AMINO ACID; BIOENGINEERING; CATALYSIS; CELLULOSE; ENZYME ACTIVITY; HYDROLYSIS; YEAST;

ARTICLE; CELL CULTURE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME PURIFICATION; GENE SEQUENCE; NONHUMAN; PAENIBACILLUS; PAENIBACILLUS FUKUINENSIS; PROTEIN HYDROLYSIS; PROTON TRANSPORT; YEAST CELL; AMINO ACID SUBSTITUTION; CHEMICAL STRUCTURE; ENZYMOLOGY; GENETICS; METABOLISM; NUCLEOTIDE SEQUENCE; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS;

PAENIBACILLUS FUKUINENSIS;

AMINO ACID SUBSTITUTION; ASPARAGINE; BACTERIAL PROTEINS; CHITOSAN; DNA MUTATIONAL ANALYSIS; GLUCANS; GLUTAMINE; GLYCOSIDE HYDROLASES; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PAENIBACILLUS; PROTEIN STRUCTURE, TERTIARY; PROTONS;

EID: 73349112256     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2041-5     Document Type: Article

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