Increase in bioluminescence intensity of firefly luciferase using genetic modification

Analytical Biochemistry

Volumn 366, Issue 2, 2007, Pages 131-136

  Fujii, Hiroya ^a   Noda, Kenichi ^a   Asami, Yasuo ^b   Kuroda, Akio ^b   Sakata, Minoru ^a   Tokida, Akihiko ^a  

^a Bussan Nanotech Research Institute Inc   (Japan)

^b HIROSHIMA UNIVERSITY   (Japan)

Author keywords

Bioluminescence; Firefly luciferase; Luminescence intensity; Mutation; Substrate affinity; Turnover rate

Indexed keywords

AMINO ACIDS; GENE EXPRESSION; PEPTIDES; PHOSPHORESCENCE; PLANTS (BOTANY);

FIREFLY LUCIFERASE; LUMINESCENCE INTENSITY; MUTATION; SUBSTRATE AFFINITY; TURNOVER RATE;

BIOLUMINESCENCE;

ARGININE; ASPARTIC ACID; FIREFLY LUCIFERASE; ISOLEUCINE; LEUCINE;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; BIOLUMINESCENCE; CARBOXY TERMINAL SEQUENCE; DNA MODIFICATION; ENZYME ACTIVITY; ENZYME PURIFICATION; GENE MUTATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN METABOLISM;

PHOTINUS PYRALIS;

EID: 34250176165     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.04.018     Document Type: Article

References (25)

1. Ford S.R., and Leach F.R. Improvements in the application of firefly luciferase assays. In: LaRossa R.A. (Ed). Bioluminescence Methods and Protocols. Methods Mol. Biol Vol. 102 (1998), Humana Press, Totowa, NJ 3-20
2. Viviani V.R. The origin, diversity, and structure function relationships of insect luciferases. Cell. Mol. Life Sci. 59 (2002) 1833-1850
3. Deluca M. Firefly luciferase. Adv. Enzymol. 44 (1976) 37-68
4. Seliger H.H., and McElroy W.D. Spectral emission and quantum yield of firefly bioluminescence. Arch. Biochem. Biophys. 88 (1960) 136-141
5. Airth R.L., Rhodes W.C., and McElroy W.D. The function of coenzyme A in luminescence. Biochim. Biophys. Acta 27 (1958) 519-532
6. Kricka L.J., and DeLuca M. Effect of solvents on the catalytic activity of firefly (Photinus pyralis) luciferase. Arch. Biochem. Biophys. 217 (1982) 674-681
7. Ford S.R., Hall M.S., and Leach F.R. Enhancement of firefly luciferase activity by cytidine nucleotides. Anal. Biochem. 204 (1992) 283-291
8. Hattori N., Sakakibara T., Kajiyama N., Igarashi T., Maeda M., and Murakami S. Enhanced microbial biomass assay using mutant luciferase resistant to benzalkonium chloride. Anal. Biochem. 319 (2003) 287-295
9. Gould S.J., and Subramani S. Firefly luciferase as a tool in molecular and cell biology. Anal. Biochem. 175 (1988) 5-13
10. Conti E., Franks N.P., and Brick P. Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes. Structure 4 (1996) 287-298
11. Branchini B.R., Magyar R.A., Marcantonio K.M., Newberry K.J., Stroh J.G., Hinz L.K., and Murtiashaw M.H. Identification of a firefly luciferase active site peptide using a benzophenone-based photooxidation reagent. J. Biol. Chem. 272 (1997) 19359-19364
12. Branchini B.R., Magyar R.A., Murtiashaw M.H., Anderson S.M., and Zimmer M. Site-directed mutagenesis of histidine 245 in firefly luciferase: a proposed model of the active site. Biochemistry 37 (1998) 15311-15319
13. Branchini B.R., Magyar R.A., Murtiashaw M.H., and Portier N.C. The role of active site residue arginine 218 in firefly luciferase bioluminescence. Biochemistry 40 (2001) 2410-2418
14. Nakatsu T., Ichiyama S., Hiratake J., Saldanha A., Kobashi N., Sakata K., and Kato H. Structural basis for the spectral difference in luciferase bioluminescence. Nature 440 (2006) 372-376
15. Sandalova T.P., and Ugarova N.N. Model of the active site of firefly luciferase. Biochemistry (Moscow) 64 (1999) 962-967
16. Branchini B.R., Murtiashaw M.H., Magyar R.A., and Anderson S.M. The role of lysine 529, a conserved residue of the acyl-adenylate-forming enzyme superfamily, in firefly luciferase. Biochemistry 39 (2000) 5433-5440
17. Branchini B.R., Southworth T.L., Murtiashaw M.H., Magyar R.A., Gonzalez S.A., Ruggiero M.C., and Stroh J.G. An alternative mechanism of bioluminescence color determination in firefly luciferase. Biochemistry 43 (2004) 7255-7262
18. Branchini B.R., Southworth T.L., Murtiashaw M.H., Wilkinson S.R., Khattak N.F., Rosenberg J.C., and Zimmer M. Mutagenesis evidence that the partial reactions of firefly bioluminescence are catalyzed by different conformations of the luciferase C-terminal domain. Biochemistry 44 (2005) 1385-1393
19. Ayabe K., Zako T., and Ueda H. The role of firefly luciferase C-terminal domain in efficient coupling of adenylation and oxidative steps. FEBS Lett. 579 (2005) 4389-4394
20. Branchini B.R., Magyar R.A., Murtiashaw M.H., Anderson S.M., Helgerson L.C., and Zimmer M. Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color. Biochemistry 38 (1999) 13223-13230
21. Kajiyama N., and Nakano E. Thermostabilization of firefly luciferase by a single amino acid substitution at position 217. Biochemistry 32 (1993) 13795-13799
22. Hattori N., Kajiyama N., Maeda M., and Murakami S. Mutant luciferase enzymes from fireflies with increased resistance to benzalkonium chloride. Biosci. Biotechnol. Biochem. 66 (2002) 2587-2593
23. Thompson J.F., Geoghegan K.F., Lloyd D.B., Lanzetti A.J., Magyar R.A., Anderson S.M., and Branchini B.R. Mutation of a protease-sensitive region in firefly luciferase alters light emission properties. J. Biol. Chem. 272 (1997) 18766-18771
24. Hirokawa K., Kajiyama N., and Murakami S. Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis. Biochim. Biophys. Acta 1597 (2002) 271-279
25. Branchini B.R., Southworth T.L., Murtiashaw M.H., Boije H., and Fleet S.E. A mutagenesis study of the putative luciferin binding site residues of firefly luciferase. Biochemistry 42 (2003) 10429-10436