Comparison of immunoassay screening tests and LC-MS-MS for urine detection of benzodiazepines and their metabolites: Results of a national proficiency test

Journal of Analytical Toxicology

Volumn 37, Issue 9, 2013, Pages 659-664

  Bertol, Elisabetta ^a   Vaiano, Fabio ^a   Borsotti, Maurizio ^b   Quercioli, Massimo ^b   Mari, Francesco ^a  

^a UNIVERSITY OF FLORENCE   (Italy)

^b CAREGGI UNIVERSITY HOSPITAL   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

7 AMINO 1,3 DIHYDRO 5 PHENYL 2H 1,4 BENZODIAZEPIN 2 ONE; 7 AMINOFLUNITRAZEPAM; ALPRAZOLAM; BENZODIAZEPINE DERIVATIVE; BROMAZEPAM; BROTIZOLAM; CHLORDIAZEPOXIDE; CLONAZEPAM; DELORAZEPAM; DIAZEPAM; DRUG METABOLITE; FLUNITRAZEPAM; HALAZEPAM; KETAZOLAM; LORAZEPAM; LORMETAZEPAM; MIDAZOLAM; NITRAZEPAM; NORDAZEPAM; OXAZEPAM; PINAZEPAM; PRAZEPAM; TEMAZEPAM; TRIAZOLAM;

ARTICLE; BIOTRANSFORMATION; CLONED ENZYME DONOR IMMUNOASSAY; COMPARATIVE STUDY; CONTROLLED STUDY; DRUG STRUCTURE; ENZYME MULTIPLIED IMMUNOASSAY TECHNIQUE; FLUORESCENCE POLARIZATION IMMUNOASSAY; FORENSIC TOXICOLOGY; HYDROLYSIS; IMMUNOASSAY; KINETIC INTERACTION OF MICROPARTICLE IN SOLUTION TECHNIQUE; LIMIT OF DETECTION; LIQUID CHROMATOGRAPHY; POLARIZATION; PREDICTIVE VALUE; SCREENING TEST; TANDEM MASS SPECTROMETRY; URINALYSIS;

BENZODIAZEPINES; BIOTRANSFORMATION; CHROMATOGRAPHY, LIQUID; FORENSIC TOXICOLOGY; HUMANS; HYDROLYSIS; IMMUNOASSAY; INDICATORS AND REAGENTS; SUBSTANCE ABUSE DETECTION; TANDEM MASS SPECTROMETRY;

EID: 84885708855     PISSN: 01464760     EISSN: 19452403     Source Type: Journal    
DOI: 10.1093/jat/bkt063     Document Type: Article

References (18)

1. Garretty, D.J., Wolff, K., Hay, A.W.M., Raistrick, D. (1997) Benzodiazepine misuse by drug addicts. Annals of Clinical Biochemistry, 34, 68-73.
2. Drummer, O.H., Randon, D.L. (1996) Sudden death and benzodiazepines. The American Journal of Forensic Medicine and Pathology, 17, 336-342.
3. Mari, F., Defraia, B., Rensi, R., Gualco, B., Vaiano, F., Bertol, E. (2013) Fatal poisoning due to co-ingestion of benzodiazepines and alcohol: a case report. Indian Journal of Forensic Medicine and Toxicology, 7, 254-287.
4. Gueye, P.N., Lofaso, F., Borron, S.W., Mellero, F., Vicaut, E., Harf, A. et al. (2002) Mechanism of respiratory insufficiency in pure or mixed drug-induced coma involving benzodiazepines. Clinical Toxicology, 40, 35-47.
5. Adams, R.H. (1986) An accident and emergency department's view of self-poisoning: a retrospective study from United Norwich Hospitals. Human Toxicology, 5, 5-10.
6. Kadjalainen, K., Lintonen, T., Impinen, A., Mäkel, P., Rahkonen, O., Lillsunde, P. et al. (2010) Mortality and causes of death among drugged drivers. Journal of Emidemiology and Community Health, 64, 506-512.
7. Schwartz, R.H., Milteer, R., LeBeau, M.A. (2000) Drug-facilitated sexual assault. Southern Medical Journal, 93, 58-61.
8. ElSohly, M.A., Gul, W., Murphy, T.P., Avula, B., Khan, I.A. (2007) LC-(TOF) MS analysis of benzodiazepines in urine from alleged victims of drug-facilitated sexual assault. Journal of Analytical Toxicology, 1, 505-514.
9. Dowd, S.M., Strong, M.J., Janicak, P.G., Negrusk, A. (2002) The behavioral and cognitive effects of two benzodiazepines associated with drug-facilitated sexual assault. Journal of Forensic Sciences, 47, 1101-1107.
10. Steward, S.H., Westra, H.A. (2002) Benzodiazepine side-effects: from the bench to the clinic. Current Pharmaceutical Design, 8, 1-3.
11. Mandrioli, R., Mercolini, L., Raggi, M.A. (2008) Benzodiazepine metabolism: an analytical perspective. Current Drug Metabolism, 9, 827-844.
12. Spiehler, V. (2000) Hair analysis by immunological methods from the beginning to 2000. Forensic Science International, 107, 249-259.
13. Mearrick, P.T., Chahl, J.S. (1985) Screening for benzodiazepines in urine after hydrolysis of glucuronide conjugates. Clinical Chemistry, 31, 152-153.
14. Meatherall, R. (1994) Benzodiazepine screening using EMIT II and TDx: urine hydrolysis pretreatment required. Journal of Analytical Toxicology, 18, 385-390.
15. Bertol, E., Vaiano, F., Furlanetto, S., Mari, F. Cross-reactivities and structure-reactivity relationships of six benzodiazepines to EMITw immunoassay. Journal of Pharmaceutical and Biomedical Analysis, http://dx.doi.org/10.1016/j.jpba.2013.05.026.
16. De Rienz, R.T., Holler, J.M., Manos, M.E., Jemionek, J., Past, M.R. (2008) Evaluation of four immunoassay screening kits for the detection of benzodiazepines in urine. Journal of Analytical Toxicology, 32, 433-437.
17. Fu, S., Lewis, J., Wang, H., Keegan, J., Dawson, M. (2010) A novel reductive transformation of oxazepam to nordiazepam observed during enzymatic hydrolysis. Journal of Analytical Toxicology, 34, 244-251.
18. Fu, S., Molnar, A., Bowron, P., Lewis, J., Wang, H. (2011) Reduction of temazepam to diazepam and lorazepam to delorazepam during enzymatic hydrolysis. Analytical and Bioanalytical Chemistry, 400, 153-164.