Actin stress fibre subtypes in mesenchymal-migrating cells

Open Biology

Volumn 3, Issue JUN, 2013, Pages

  Vallenius, Tea ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Actin; Dorsal stress fibres; Mesenchymal; Migration; Transverse arcs; Ventral stress fibres

Indexed keywords

ACTIN; MYOSIN II; VINCULIN;

CELL MOTION; CYTOLOGY; DORSAL STRESS FIBRES; EXTRACELLULAR MATRIX; HUMAN; MESENCHYMAL; MESENCHYMAL STROMA CELL; METABOLISM; MIGRATION; REVIEW; STRESS FIBER; TRANSVERSE ARCS; VENTRAL STRESS FIBRES;

ACTIN; DORSAL STRESS FIBRES; MESENCHYMAL; MIGRATION; TRANSVERSE ARCS; VENTRAL STRESS FIBRES;

ACTINS; CELL MOVEMENT; EXTRACELLULAR MATRIX; HUMANS; MESENCHYMAL STROMAL CELLS; MYOSIN TYPE II; STRESS FIBERS; VINCULIN;

EID: 84885654506     PISSN: None     EISSN: 20462441     Source Type: Journal    
DOI: 10.1098/rsob.130001     Document Type: Article

References (100)

1. Lewis WH, Lewis MR. 1924 Behavior of cells in tissue cultures. In General Cytology (ed. EV Cowdry), pp. 385-447. Chicago, IL: The University of Chicago Press
2. Buckley IK, Porter KR. 1967 Cytoplasmic fibrils in living cultured cells. A light and electron microscope study. Protoplasma 64, 349-380. (doi:10.1007/ BF01666538)
3. Gabbiani G, Gabbiani F, Lombardi D, Schwartz SM. 1983 Organization of actin cytoskeleton in normal and regenerating arterial endothelial cells. Proc. Natl Acad. Sci. USA 80, 2361-2364. (doi:10.1073/ pnas.80.8.2361) (Pubitemid 13097189)
4. Thery M, Pepin A, Dressaire E, Chen Y, Bornens M. 2006 Cell distribution of stress fibres in response to the geometry of the adhesive environment. Cell Motil. Cytoskeleton 63, 341-355. (doi:10.1002/ cm.20126) (Pubitemid 43787719)
5. Yeung T et al. 2005 Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion. Cell Motil. Cytoskeleton 60, 24-34. (doi:10.1002/cm.20041) (Pubitemid 40066224)
6. Clarke M, Spudich JA. 1977 Nonmuscle contractile proteins: the role of actin and myosin in cell motility and shape determination. Annu. Rev. Biochem. 46, 797-822. (doi:10.1146/annurev.bi.46. 070177.004053)
7. Ishikawa H, Bischoff R, Holtzer H. 1969 Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J. Cell Biol. 43, 312-328. (doi:10.1083/jcb.43.2.312)
8. Pollard TD. 1981 Cytoplasmic contractile proteins. J. Cell Biol. 91, 156s-165s. (doi:10.1083/jcb.91. 3.156s) (Pubitemid 12177867)
9. Vasiliev JM, Gelfand IM. 1977 Mechanisms of morphogenesis in cell cultures. Int. Rev. Cytol. 50, 159-274. (doi:10.1016/S0074-7696(08)60099-6) (Pubitemid 8173562)
10. Lazarides E, Weber K. 1974 Actin antibody: the specific visualization of actin filaments in nonmuscle cells. Proc. Natl Acad. Sci. USA 71, 2268-2272. (doi:10.1073/pnas.71.6.2268)
11. Fujiwara K, Pollard TD. 1976 Fluorescent antibody localization of myosin in the cytoplasm, cleavage furrow, and mitotic spindle of human cells. J. Cell Biol. 71, 848-875. (doi:10.1083/jcb.71.3.848)
12. Weber K, Groeschel-Stewart U. 1974 Antibody to myosin: the specific visualization of myosincontaining filaments in nonmuscle cells. Proc. Natl Acad. Sci. USA 71, 4561-4564. (doi:10.1073/pnas. 71.11.4561)
13. Lazarides E. 1975 Tropomyosin antibody: the specific localization of tropomyosin in nonmuscle cells. J. Cell Biol. 65, 549-561. (doi:10.1083/jcb. 65.3.549)
14. Lazarides E, Burridge K. 1975 α-actinin: immunofluorescent localization of a muscle structural protein in nonmuscle cells. Cell 6, 289-298. (doi:10.1016/0092-8674(75)90180-4)
15. Honda K, Yamada T, Endo R, Ino Y, Gotoh M, Tsuda H, Yamada Y, Chiba H, Hirohashi S. 1998 Actinin-4, a novel actin-bundling protein associated with cell motility and cancer invasion. J. Cell Biol. 140, 1383-1393. (doi:10.1083/jcb.140.6.1383) (Pubitemid 28152987)
16. Kawamoto S, Adelstein RS. 1991 Chicken nonmuscle myosin heavy chains: differential expression of two mRNAs and evidence for two different polypeptides. J. Cell Biol. 112, 915-924. (doi:10.1083/jcb.112. 5.915) (Pubitemid 21909638)
17. Youssoufian H, McAfee M, Kwiatkowski DJ. 1990 Cloning and chromosomal localization of the human cytoskeletal alphα-actinin gene reveals linkage to the betα-spectrin gene. Am. J. Hum. Genet. 47, 62-72.
18. Burridge K, Chrzanowskα-Wodnicka M. 1996 Focal adhesions, contractility, and signaling. Annu. Rev. Cell Dev. Biol. 12, 463-518. (doi:10.1146/annurev. cellbio.12.1.463)
19. Giuliano KA, Taylor DL. 1990 Formation, transport, contraction, and disassembly of stress fibers in fibroblasts. Cell Motil. Cytoskeleton 16, 14-21. (doi:10.1002/cm.970160104)
20. Harris AK, Wild P, Stopak D. 1980 Silicone rubber substrata: a new wrinkle in the study of cell locomotion. Science 208, 177-179. (doi:10.1126/ science.6987736) (Pubitemid 10072133)
21. Isenberg G, Rathke PC, Hulsmann N, Franke WW, Wohlfarth-Bottermann KE. 1976 Cytoplasmic actomyosin fibrils in tissue culture cells: direct proof of contractility by visualization of ATP-induced contraction in fibrils isolated by laser micro-beam dissection. Cell Tissue Res. 166, 427-443. (doi:10. 1007/BF00225909)
22. Kreis TE, Birchmeier W. 1980 Stress fiber sarcomeres of fibroblasts are contractile. Cell 22, 555-561. (doi:10.1016/0092-8674(80)90365-7) (Pubitemid 11199912)
23. Ono S. 2010 Dynamic regulation of sarcomeric actin filaments in striated muscle. Cytoskeleton (Hoboken) 67, 677-692. (doi:10.1002/cm.20476)
24. Goldman RD, Chojnacki B, Yerna MJ. 1979 Ultrastructure of microfilament bundles in baby hamster kidney (BHK-21) cells. The use of tannic acid. J. Cell Biol. 80, 759-766. (doi:10.1083/jcb. 80.3.759) (Pubitemid 9136049)
25. Sanger JM, Sanger JW. 1980 Banding and polarity of actin filaments in interphase and cleaving cells. J. Cell Biol. 86, 568-575. (doi:10.1083/jcb.86.2. 568) (Pubitemid 10016278)
26. Cramer LP, Siebert M, Mitchison TJ. 1997 Identification of novel graded polarity actin filament bundles in locomoting heart fibroblasts: implications for the generation of motile force. J. Cell Biol. 136, 1287-1305. (doi:10.1083/jcb.136. 6.1287) (Pubitemid 27421916)
27. Svitkina TM, Verkhovsky AB, McQuade KM, Borisy GG. 1997 Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation. J. Cell Biol. 139, 397-415. (doi:10. 1083/jcb.139.2.397) (Pubitemid 27459313)
28. Pellegrin SaM H. 2007 Actin stress fibers. J. Cell Sci. 120, 3491-3499. (doi:10.1242/jcs.018473) (Pubitemid 350120942)
29. Ang SF, Zhao ZS, Lim L, Manser E. 2010 DAAM1 is a formin required for centrosome re-orientation during cell migration. PLoS ONE 5, e13064. (doi:10. 1371/journal.pone.0013064)
30. Hotulainen P, Lappalainen P. 2006 Stress fibers are generated by two distinct actin assembly mechanisms in motile cells. J. Cell Biol. 173, 383-394. (doi:10.1083/jcb.200511093)
31. Kovac B, Teo JL, Makela TP, Vallenius T. 2013 Assembly of non-contractile dorsal stress fibers requires alphα-actinin-1 and Rac1 in migrating and spreading cells. J. Cell Sci. 126, 263-273. (doi:10. 1242/jcs.115063)
32. Tojkander S, Gateva G, Lappalainen P. 2012 Actin stress fibers: assembly, dynamics and biological roles. J. Cell Sci. 125, 1855-1864. (doi:10.1242/jcs.098087)
33. Tojkander S, Gateva G, Schevzov G, Hotulainen P, Naumanen P, Martin C, Gunning PW, Lappalainen P. 2011 A molecular pathway for myosin II recruitment to stress fibers. Curr. Biol. 21, 539-550. (doi:10. 1016/j.cub.2011.03.007)
34. Friedl P, Wolf K. 2003 Tumour-cell invasion and migration: diversity and escape mechanisms. Nat. Rev. Cancer 3, 362-374. (doi:10.1038/nrc1075) (Pubitemid 37328856)
35. Friedl P, Wolf K. 2010 Plasticity of cell migration: a multiscale tuning model. J. Cell Biol. 188, 11-19. (doi:10.1083/jcb.200909003)
36. Madsen CD, Sahai E. 2010 Cancer dissemination - lessons from leukocytes. Dev. Cell 19, 13-26. (doi:10.1016/j.devcel.2010.06.013)
37. Sixt M. 2012 Cell migration: fibroblasts find a new way to get ahead. J. Cell Biol. 197, 347-349. (doi:10.1083/jcb.201204039)
38. Abercrombie M, Heaysman JE, Pegrum SM. 1970 The locomotion of fibroblasts in culture. III. Movements of particles on the dorsal surface of the leading lamella. Exp. Cell Res. 62, 389-398. (doi:10.1016/0014-4827(70)90570-7)
39. Abercrombie M, Heaysman JE, Pegrum SM. 1970 The locomotion of fibroblasts in culture. II. 'RRuffling'. Exp. Cell Res. 60, 437-444. (doi:10. 1016/0014-4827(70)90537-9)
40. Abercrombie M, Heaysman JE, Pegrum SM. 1970 The locomotion of fibroblasts in culture. I. Movements of the leading edge. Exp. Cell Res. 59, 393-398. (doi:10.1016/0014- 4827(70)90646-4)
41. Lauffenburger DA, Horwitz AF. 1996 Cell migration: a physically integrated molecular process. Cell 84, 359-369. (doi:10.1016/S0092- 8674(00)81280-5) (Pubitemid 26058561)
42. Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, Parsons JT, Horwitz AR. 2003 Cell migration: integrating signals from front to back. Science 302, 1704-1709. (doi:10.1126/ science.1092053) (Pubitemid 37505725)
43. Burridge K. 1981 Are stress fibres contractile? Nature 294, 691-692. (doi:10.1038/294691a0)
44. Kalluri R, Weinberg RA. 2009 The basics of epithelial-mesenchymal transition. J. Clin. Invest. 119, 1420-1428. (doi:10.1172/JCI39104)
45. Thiery JP, Acloque H, Huang RY, Nieto MA. 2009 Epithelial-mesenchymal transitions in development and disease. Cell 139, 871-890. (doi:10.1016/j.cell. 2009.11.007)
46. Small JV, Rottner K, Kaverina I, Anderson KI. 1998 Assembling an actin cytoskeleton for cell attachment and movement. Biochim. Biophys. Acta 1404, 271-281. (doi:10.1016/S0167- 4889(98)00080-9) (Pubitemid 28470529)
47. Oakes PW, Beckham Y, Stricker J, Gardel ML. 2012 Tension is required but not sufficient for focal adhesion maturation without a stress fiber template. J. Cell Biol. 196, 363-374. (doi:10.1083/jcb.201107042)
48. Heath JP. 1983 Behaviour and structure of the leading lamella in moving fibroblasts. I. Occurrence and centripetal movement of arc-shaped microfilament bundles beneath the dorsal cell surface. J. Cell Sci. 60, 331-354.
49. Aratyn-Schaus Y, Oakes PW, Gardel ML. 2011 Dynamic and structural signatures of lamellar actomyosin force generation. Mol. Biol. Cell 22, 1330-1339. (doi:10.1091/mbc.E10-11-0891)
50. Nemethova M, Auinger S, Small JV. 2008 Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella. J. Cell Biol. 180, 1233-1244. (doi:10.1083/jcb. 200709134) (Pubitemid 351468477)
51. Li B, Zhuang L, Reinhard M, Trueb B. 2003 The lipoma preferred partner LPP interacts with α-actinin. J. Cell Sci. 116, 1359-1366. (doi:10. 1242/jcs.00309) (Pubitemid 36410673)
52. Mukai H, Toshimori M, Shibata H, Takanaga H, Kitagawa M, Miyahara M, Shimakawa M, Ono Y. 1997 Interaction of PKN with alphα-actinin. J. Biol. Chem. 272, 4740-4746. (doi:10.1074/jbc.272. 8.4740)
53. Pomies P, Louis HA, Beckerle MC. 1997 CRP1, a LIM domain protein implicated in muscle differentiation, interacts with α-actinin. J. Cell Biol. 139, 157-168. (doi:10.1083/jcb.139.1.157) (Pubitemid 27444088)
54. Vallenius T, Luukko K, Mäkelä TP. 2000 CLP-36 PDZLIM protein associates with nonmuscle α-actinin-1 and alphα-actinin-4. J. Biol. Chem. 275, 11 100- 11 105. (doi:10.1074/jbc.275.15.11100) (Pubitemid 30212752)
55. Vallenius T, Scharm B, Vesikansa A, Luukko K, Schafer R, Makela TP. 2004 The PDZ-LIM protein RIL modulates actin stress fiber turnover and enhances the association of α-actinin with F-actin. Exp. Cell Res. 293, 117-128. (doi:10.1016/j.yexcr.2003. 09.004) (Pubitemid 38064419)
56. Craig DH, Haimovich B, Basson MD. 2007 Alphaactinin- 1 phosphorylation modulates pressureinduced colon cancer cell adhesion through regulation of focal adhesion kinase-Src interaction. Am. J. Physiol. Cell Physiol. 293, C1862-C1874. (doi:10.1152/ajpcell.00118.2007) (Pubitemid 350255082)
57. Izaguirre G, Aguirre L, Hu YP, Lee HY, Schlaepfer DD, Aneskievich BJ, Haimovich B. 2001 The cytoskeletal/ non-muscle isoform of α-actinin is phosphorylated on its actin-binding domain by the focal adhesion kinase. J. Biol. Chem. 276, 28 676-28 685. (doi:10. 1074/jbc.M101678200)
58. Shao H, Wu C, Wells A. 2010 Phosphorylation of alphα-actinin 4 upon epidermal growth factor exposure regulates its interaction with actin. J. Biol. Chem. 285, 2591-2600. (doi:10.1074/jbc.M109. 035790)
59. Parsons JT, Horwitz AR, Schwartz MA. 2010 Cell adhesion: integrating cytoskeletal dynamics and cellular tension. Nat. Rev. Mol. Cell Biol. 11, 633-643. (doi:10.1038/nrm2957)
60. Vicente-Manzanares M, Ma X, Adelstein RS, Horwitz AR. 2009 Non-muscle myosin II takes centre stage in cell adhesion and migration. Nat. Rev. Mol. Cell Biol. 10, 778-790. (doi:10.1038/ nrm2786)
61. Kolega J. 1998 Cytoplasmic dynamics of myosin IIA and IIB: spatial 'sorting' of isoforms in locomoting cells. J. Cell Sci. 111, 2085-2095. (Pubitemid 28403514)
62. Lo CM, Buxton DB, Chua GC, Dembo M, Adelstein RS, Wang YL. 2004 Nonmuscle myosin IIb is involved in the guidance of fibroblast migration. Mol. Biol. Cell 15, 982-989. (doi:10.1091/mbc.E03- 06-0359) (Pubitemid 38316209)
63. Maupin P, Phillips CL, Adelstein RS, Pollard TD. 1994 Differential localization of myosin-II isozymes in human cultured cells and blood cells. J. Cell Sci. 107, 3077-3090. (Pubitemid 24365211)
64. Saitoh T, Takemura S, Ueda K, Hosoya H, Nagayama M, Haga H, Kawabata K, Yamagishi A, Takahashi M. 2001 Differential localization of non-muscle myosin II isoforms and phosphorylated regulatory light chains in human MRC-5 fibroblasts. FEBS Lett. 509, 365-369. (doi:10.1016/S0014-5793(01)03186-6) (Pubitemid 34031962)
65. Vicente-Manzanares M, Koach MA, Whitmore L, Lamers ML, Horwitz AF. 2008 Segregation and activation of myosin IIB creates a rear in migrating cells. J. Cell Biol. 183, 543-554. (doi:10.1083/jcb. 200806030)
66. Vicente-Manzanares M, Zareno J, Whitmore L, Choi CK, Horwitz AF. 2007 Regulation of protrusion, adhesion dynamics, and polarity by myosins IIA and IIB in migrating cells. J. Cell Biol. 176, 573-580. (doi:10.1083/jcb.200612043) (Pubitemid 46333973)
67. Bunnell TM, Burbach BJ, Shimizu Y, Ervasti JM. 2011 betα-Actin specifically controls cell growth, migration, and the G-actin pool. Mol. Biol. Cell 22, 4047-4058. (doi:10.1091/mbc.E11- 06-0582)
68. Tondeleir D et al. 2012 Cells lacking β-actin are genetically reprogrammed and maintain conditional migratory capacity. Mol. Cell Proteomics 11, 255-271. (doi:10.1074/mcp.M111.015099)
69. Katz ZB, Wells AL, Park HY, Wu B, Shenoy SM, Singer RH. 2012 β-Actin mRNA compartmentalization enhances focal adhesion stability and directs cell migration. Genes Dev. 26, 1885-1890. (doi:10.1101/gad.190413.112)
70. Kislauskis EH, Zhu X, Singer RH. 1997 betα-Actin messenger RNA localization and protein synthesis augment cell motility. J. Cell Biol. 136, 1263-1270. (doi:10.1083/jcb.136.6.1263) (Pubitemid 27421914)
71. Geiger B, Yamada KM. 2011 Molecular architecture and function of matrix adhesions. Cold Spring Harb. Perspect. Biol. 3, a005033. (doi:10.1101/ cshperspect. a005033)
72. Schwartz MA. 2010 Integrins and extracellular matrix in mechanotransduction. Cold Spring Harb. Perspect. Biol. 2, a005066. (doi:10.1101/cshperspect.a005066)
73. Vicente-Manzanares M, Newell-Litwa K, Bachir AI, Whitmore LA, Horwitz AR. 2011 Myosin IIA/IIB restrict adhesive and protrusive signaling to generate front-back polarity in migrating cells. J. Cell Biol. 193, 381-396. (doi:10.1083/jcb. 201012159)
74. Chrzanowskα-Wodnicka M, Burridge K. 1996 Rhostimulated contractility drives the formation of stress fibers and focal adhesions. J. Cell Biol. 133, 1403-1415. (doi:10.1083/jcb.133.6.1403) (Pubitemid 26192339)
75. Cai Y, Sheetz MP. 2009 Force propagation across cells: mechanical coherence of dynamic cytoskeletons. Curr. Opin. Cell Biol. 21, 47-50. (doi:10.1016/j.ceb.2009.01.020)
76. Puklin-Faucher E, Sheetz MP. 2009 The mechanical integrin cycle. J. Cell Sci. 122, 179-186. (doi:10. 1242/jcs.042127)
77. Geiger B, Spatz JP, Bershadsky AD. 2009 Environmental sensing through focal adhesions. Nat. Rev. Mol. Cell Biol. 10, 21-33. (doi:10.1038/ nrm2593)
78. Choi CK, Vicente-Manzanares M, Zareno J, Whitmore LA, Mogilner A, Horwitz AR. 2008 Actin and α-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motorindependent manner. Nat. Cell Biol. 10, 1039-1050. (doi:10.1038/ncb1763)
79. Kuo JC, Han X, Hsiao CT, Yates 3rd JR, Waterman CM. 2011 Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for betα-Pix in negative regulation of focal adhesion maturation. Nat. Cell Biol. 13, 383-393. (doi:10.1038/ncb2216)
80. Pasapera AM, Schneider IC, Rericha E, Schlaepfer DD, Waterman CM. 2010 Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation. J. Cell Biol. 188, 877-890. (doi:10.1083/jcb.200906012)
81. Schiller HB, Friedel CC, Boulegue C, Fassler R. 2011 Quantitative proteomics of the integrin adhesome show a myosin II-dependent recruitment of LIM domain proteins. EMBO Rep. 12, 259-266. (doi:10. 1038/embor.2011.5)
82. Margadant F, Chew LL, Hu X, Yu H, Bate N, Zhang X, Sheetz M. 2011 Mechanotransduction in vivo by repeated talin stretch-relaxation events depends upon vinculin. PLoS Biol. 9, e1001223. (doi:10.1371/ journal.pbio.1001223)
83. Sawada Y, Tamada M, Dubin-Thaler BJ, Cherniavskaya O, Sakai R, Tanaka S, Sheetz MP. 2006 Force sensing by mechanical extension of the Src family kinase substrate p130Cas. Cell 127, 1015-1026. (doi:10.1016/j.cell.2006.09.044) (Pubitemid 44803062)
84. Zamir E et al. 2000 Dynamics and segregation of cell-matrix adhesions in cultured fibroblasts. Nat. Cell Biol. 2, 191-196. (doi:10.1038/35008607)
85. Riveline D, Zamir E, Balaban NQ, Schwarz US, Ishizaki T, Narumiya S, Kam Z, Geiger B, Bershadsky AD. 2001 Focal contacts as mechanosensors: externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCK-independent mechanism. J. Cell Biol. 153, 1175-1186. (doi:10.1083/jcb.153.6.1175) (Pubitemid 34289257)
86. Lo CM, Wang HB, Dembo M, Wang YL. 2000 Cell movement is guided by the rigidity of the substrate. Biophys. J. 79, 144-152. (doi:10.1016/S0006- 3495(00)76279-5) (Pubitemid 30436733)
87. Raab M, Swift J, PC PD, Shah P, Shin JW, Discher DE. 2012 Crawling from soft to stiff matrix polarizes the cytoskeleton and phosphoregulates myosin-II heavy chain. J. Cell Biol. 199, 669-683. (doi:10. 1083/jcb.201205056)
88. Ridley AJ. 2011 Life at the leading edge. Cell 145, 1012-1022. (doi:10.1016/j.cell.2011.06.010)
89. Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn KM. 2009 A genetically encoded photoactivatable Rac controls the motility of living cells. Nature 461, 104-108. (doi:10.1038/ nature08241)
90. Machacek M et al. 2009 Coordination of Rho GTPase activities during cell protrusion. Nature 461, 99-103. (doi:10.1038/nature08242)
91. Kozma R, Sarner S, Ahmed S, Lim L. 1997 Rho family GTPases and neuronal growth cone remodelling: relationship between increased complexity induced by Cdc42Hs, Rac1, and acetylcholine and collapse induced by RhoA and lysophosphatidic acid. Mol. Cell Biol. 17, 1201-1211. (Pubitemid 27097349)
92. Katoh K, Kano Y, Amano M, Kaibuchi K, Fujiwara K. 2001 Stress fiber organization regulated by MLCK and Rho-kinase in cultured human fibroblasts. Am. J. Physiol. Cell Physiol. 280, C1669-C1679.
93. Katoh K, Kano Y, Amano M, Onishi H, Kaibuchi K, Fujiwara K. 2001 Rho-kinase-mediated contraction of isolated stress fibers. J. Cell Biol. 153, 569-584. (doi:10.1083/jcb.153.3.569)
94. Vallenius T, Vaahtomeri K, Kovac B, Osiceanu AM, Viljanen M, Makela TP. 2011 An associationbetween NUAK2 and MRIP reveals a novel mechanism for regulation of actin stress fibers. J. Cell Sci. 124, 384-393. (doi:10.1242/jcs.072660)
95. Wilkinson S, Paterson HF, Marshall CJ. 2005 Cdc42- MRCK and Rho-ROCK signalling cooperate in myosinphosphorylation and cell invasion. Nat. Cell Biol. 7, 255-261. (doi:10.1038/ncb1230) (Pubitemid 40331126)
96. Fischer RS, Gardel M, Ma X, Adelstein RS, WatermanCM. 2009 Local cortical tension by myosin II guides 3D endothelial cell branching. Curr. Biol. 19, 260-265. (doi:10.1016/j.cub.2008.12.045)
97. Friedl P, Sahai E, Weiss S, Yamada KM. 2012 New dimensions in cell migration. Nat. Rev. Mol. Cell Biol. 13, 743-747. (doi:10.1038/nrm3459)
98. Kubow KE, Horwitz AR. 2011 Reducing background fluorescence reveals adhesions in 3D matrices. Nat. Cell Biol. 13, 3-5 (author reply 5-7). (doi:10. 1038/ncb0111-3)
99. Riedl J et al. 2010 Lifeact mice for studying F-actin dynamics. Nat. Methods 7, 168-169. (doi:10.1038/ nmeth0310-168)
100. Giampieri S, Pinner S, Sahai E. 2010 Intravital imaging illuminates transforming growth factor beta signaling switches during metastasis. Cancer Res. 70, 3435-3439. (doi:10.1158/0008-5472.CAN-10-0466)