Use of a nuclease enzyme in the purification of VAQTA®, a hepatitis A vaccine

Biotechnology and Applied Biochemistry

Volumn 23, Issue 3, 1996, Pages 209-215

  Hagen, Anna J ^a   Aboud, Robert A ^a   DePhillips, Peter A ^a   Oliver, Cynthia N ^b   Orella, Charles J ^a   Sitrin, Robert D ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

^b MEDIMMUNE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EDETIC ACID; HEPATITIS A VACCINE; INACTIVATED VIRUS VACCINE; MACROGOL; MAGNESIUM CHLORIDE; NUCLEASE; NUCLEIC ACID; SODIUM CHLORIDE; SODIUM DIHYDROGEN PHOSPHATE;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CELL LYSATE; DRUG DESIGN; DRUG MANUFACTURE; DRUG PURIFICATION; GOOD MANUFACTURING PRACTICE; HEPATITIS A VIRUS; ION EXCHANGE CHROMATOGRAPHY; THAWING; ULTRASOUND;

BINDING SITES; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; DEOXYRIBONUCLEASES; FILTRATION; HEPATITIS A VACCINES; HEPATITIS A VIRUS, HUMAN; HUMANS; NUCLEIC ACIDS; SUBSTRATE SPECIFICITY; VACCINES, INACTIVATED; VIRAL HEPATITIS VACCINES;

EID: 0029884570     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (8)

1. Gust, I.D. and Feinstone, S.M. (1988) in Hepatitis A, pp. 41-59, CRC Press, Boca Raton, FL
2. Werzberger, A., Mensch, B., Kuter, B., Brown, L., Lewis, J., Sitrin, R., Miller, W., Shouval, D., Wiens, B., Calandra, G., Ryan, J., Provost, P. and Nalin, D. (1992) N. Engl. J. Med. 327, 453-457
3. Lewis, J.A., Emini, E.A. and Armstrong, M.A. (1992) European Patent 0302692B
4. Armstrong, M.E., Giesa, P.A., Davide, J.P., Redner, F., Waterbury, J.A., Rhoad, A.E., Keys, R.D., Provost, P.J. and Lewis, J.A. (1993) J. Hepatol. 18 (suppl. 2), S20-S26
5. Aboud, R.A., Aunins, J.G., Buckland, B.B., DePhillips, P.A., Hagen, A.J., Hennessey, Jr., J.P., Junker, B., Lewis, J.A., Oliver, C.N., Orella, C.J. and Sitrin, R.D. (1994) Patent Appl. WO 94/03589 (17 February)
6. Hagen, A., Aboud, R., DePhillips, P., Orella, C., Armstrong, M., Lewis, J., Sitrin, R. and Oliver, C. (1994) Am. Chem. Soc. Natl. Meet. 207th, San Diego, March 13-17, abstr. BIOT 009
7. DePhillips, P., Orella, C. and Sitrin, R. (1993) HPLC of Proteins, Peptides and Polynucleotides: Int. Symp. 13th, San Francisco, November 30-December 3
8. Hagen, A., Oliver, C. and Sitrin, R. (1996) Biotechnol. Prog., in the press