Assembly and Isolation of Spliceosomal Complexes In Vitro

Alternative pre-mRNA Splicing: Theory and Protocols

Volumn , Issue , 2012, Pages 330-340

  Hartmuth, Klaus ^a   van Santen, Maria A ^a   Odenwälder, Peter ^a   Lührmann, Reinhard ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

Affinity selection; C complex; MS2 MBP; Spliceosome

Indexed keywords

EID: 84885522212     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9783527636778.ch31     Document Type: Chapter

References (26)

1. Frendewey, D. and Keller, W. (1985) Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences. Cell, 42, 355-367.
2. Grabowski, P.J., Seiler, S.R., and Sharp, P.A. (1985) A multicomponent complex is involved in the splicing of messenger RNA precursors. Cell, 42, 345-353.
3. Konarska, M.M. and Sharp, P.A. (1986) Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs. Cell, 46, 845-855.
4. Staley, J.P. and Guthrie, C. (1998) Mechanical devices of the spliceosome: motors, clocks, springs, and things. Cell, 92, 315-326.
5. Wahl, M.C., Will, C.L., and Lührmann, R. (2009) The spliceosome: design principles of a dynamic RNP machine. Cell, 136, 701-718.
6. Das, R. and Reed, R. (1999) Resolution of the mammalian E complex and the ATPdependent spliceosomal complexes on native agarose mini-gels. RNA, 5, 1504-1508.
7. Guthrie, C. (2010) From the ribosome to the spliceosome and back again. J. Biol. Chem., 285, 1-12.
8. Jurica, M.S. and Moore, M.J. (2002) Capturing splicing complexes to study structure and mechanism. Methods, 28, 336-345.
9. Makarov, E.M.,Makarova, O.V., Urlaub, H., Gentzel, M., Will, C.L., Wilm, M., and Lührmann, R. (2002) Small nuclear ribonucleoprotein remodeling during catalytic activation of the spliceosome. Science, 298, 2205-2208.
10. Neubauer, G., King, A., Rappsilber, J., Calvio, C.,Watson, M.,Ajuh, P., Sleeman, J., Lamond, A., and Mann, M. (1998) Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex. Nat. Genet., 20, 46-50.
11. Hartmuth, K., Urlaub, H., Vornlocher, H.P., Will, C.L., Gentzel, M., Wilm, M., and Lührmann, R. (2002) Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method. Proc. Natl Acad. Sci. USA, 99, 16719-16724.
12. Behzadnia, N., Golas, M.M., Hartmuth, K., Sander, B., Kastner, B., Deckert, J., Dube, P., Will, C.L., Urlaub, H., Stark, H. et al. (2007) Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes. EMBO J., 26, 1737-1748.
13. Zhou, Z. and Reed, R. (2003) Purification of functional RNA-protein complexes using MS2-MBP. Curr. Protoc. Mol. Biol., 27.3.1-27.3.7.
14. Das, R., Zhou, Z., and Reed, R. (2000) Functional association of U2 snRNP with theATP-independent spliceosomal complex E. Mol. Cell, 5, 779-787.
15. Zhou, Z., Licklider, L.J., Gygi, S.P., and Reed, R. (2002) Comprehensive proteomic analysis of the human spliceosome. Nature, 419, 182-185.
16. LeCuyer, K.A., Behlen, L.S., and Uhlenbeck, O.C. (1995) Mutants of the bacteriophage MS2 coat protein that alter its cooperative binding to RNA. Biochemistry, 34, 10600-10606.
17. Zhou, Z., Sim, J., Griffith, J., and Reed, R. (2002) Purification and electron microscopic visualization of functional human spliceosomes. Proc. Natl Acad. Sci. USA, 99, 12203-12207.
18. Merz, C., Urlaub, H., Will, C.L., and Lührmann, R. (2007) Protein composition of human mRNPs spliced in vitro and differential requirements formRNPprotein recruitment. RNA, 13, 116-128.
19. Deckert, J., Hartmuth, K., Boehringer, D., Behzadnia, N., Will, C.L., Kastner, B., Stark, H., Urlaub, H., and Lührmann, R. (2006) Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions. Mol. Cell. Biol., 26, 5528-5543.
20. Bessonov, S., Anokhina, M., Will, C.L., Urlaub, H., and Lührmann, R. (2008) Isolation of an active step I spliceosome and composition of its RNP core. Nature, 452, 846-850.
21. Fabrizio, P., Dannenberg, J., Dube, P., Kastner, B., Stark, H., Urlaub, H., and Luhrmann, R. (2009) The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome. Mol. Cell, 36, 593-608.
22. Warkocki, Z., Odenwalder, P., Schmitzova, J., Platzmann, F., Stark, H., Urlaub, H., Ficner, R., Fabrizio, P., and Luhrmann, R. (2009) Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components. Nat. Struct. Mol. Biol., 16, 1237-1243.
23. Krainer, A.R., Maniatis, T., Ruskin, B., and Green, M.R. (1984) Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro. Cell, 36, 993-1005.
24. Robberson, B.L., Cote, G.J., and Berget, S.M. (1990) Exon definition may facilitate splice site selection in RNAs with multiple exons. Mol. Cell. Biol., 10, 84-94.
25. Wollerton, M.C., Gooding, C., Wagner, E.J., Garcia-Blanco, M.A., and Smith, C.W. (2004) Autoregulation of polypyrimidine tract binding protein by alternative splicing leading to nonsense-mediated decay. Mol. Cell, 13, 91-100.
26. Sambrook, J. and Russel, D.W. (2001) Molecular Cloning, 3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.