Resolution of the mammalian E complex and the ATP-dependent spliceosomal complexes on native agarose mini-gels

RNA

Volumn 5, Issue 11, 1999, Pages 1504-1508

  Das, Rita ^a   Reed, Robin ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

E complex; Native gel; Spliceosome; Spliceosome assembly; Splicing

Indexed keywords

POLYACRYLAMIDE GEL;

ARTICLE; CHEMICAL ANALYSIS; COMPLEX FORMATION; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; SPLICEOSOME;

ADENOSINE TRIPHOSPHATE; ANIMALS; CHROMATOGRAPHY, GEL; ELECTROPHORESIS, AGAR GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HEPARIN; MAMMALS; RIBONUCLEOPROTEINS, SMALL NUCLEAR; RNA PRECURSORS; SPLICEOSOMES;

MAMMALIA;

EID: 0032708815     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838299991501     Document Type: Article

References (18)

1. Bennett M, Pinol-Roma S, Staknis D, Dreyfuss G, Reed R. 1992. Differential binding of heterogeneous nuclear ribonucleoproteins to mRNA precursors prior to spliceosome assembly in vitro. Mol Cell Biol 12:3165-3175.
2. Brody E, Abelson J. 1985. The "spliceosome": Yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction. Science 228:963-967.
3. Champion-Arnaud P, Gozani O, Palandjian L, Reed R. 1995. Accumulation of a novel spliceosomal complex on pre-mRNAs containing branch site mutations. Mol Cell Biol 15:5750-5756.
4. Frendewey D, Keller W. 1985. Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences. Cell 42:355-367.
5. Gozani O, Patton JG, Reed R. 1994. A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction. EMBO J 13:3356-3367.
6. Grabowski PJ, Seiler SR, Sharp PA. 1985. A multicomponent complex is involved in the splicing of messenger RNA precursors. Cell 42:345-353.
7. Jamison SF, Crow A, Garcia-Blanco MA. 1992. The spliceosome assembly pathway in mammalian extracts. Mol Cell Biol 12:4279-4287.
8. Konarska MM, Grabowski PJ, Padgett RA, Sharp PA. 1985. Characterization of the branch site in lariat RNAs produced by splicing of mRNA precursors. Nature 313:552-557.
9. Konarska MM, Sharp PA. 1986. Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs. Cell 46:845-855.
10. Konarska MM, Sharp PA. 1987. Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes. Cell 49:763-774.
11. Krainer AR, Maniatis T, Ruskin B, Green MR. 1984. Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro. Cell 36:993-1005.
12. Lamond Al, Konarska MM, Sharp PA. 1987. A mutational analysis of spliceosome assembly: Evidence for splice site collaboration during spliceosome formation. Genes & Dev 1:532-543.
13. Michaud S, Reed R. 1991. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway. Genes & Dev 5:2534-2546.
14. Michaud S, Reed R. 1993. A functional association between the 5′ and 3′ splice site is established in the earliest prespliceosome complex (E) in mammals. Genes & Dev 7:1008-1020.
15. Pikielny CW, Rymond BC, Rosbash M. 1986. Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes. Nature 324:341-345.
16. Reed R. 1990. Protein composition of mammalian spliceosomes assembled in vitro. Proc Natl Acad Sci USA 87:8031-8035.
17. Reed R, Griffith J, Maniatis T. 1988. Purification and visualization of native spliceosomes. Cell 53:949-961.
18. Séraphin B, Rosbash M. 1989. Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing. Cell 59:349-358.