Current concepts in the diagnosis of blood stream infections: Are novel molecular methods useful in clinical practice?

International Journal of Infectious Diseases

Volumn 17, Issue 11, 2013, Pages e934-e938

  Huttunen, Reetta ^a,b   Syrjänen, Jaana ^a,b   Vuento, Risto ^c   Aittoniemi, Janne ^c  

^a TAMPERE UNIVERSITY HOSPITAL   (Finland)

^b UNIVERSITY OF TAMPERE MEDICAL SCHOOL   (Finland)

^c PIRKANMAA HOSPITAL DISTRICT   (Finland)

Author keywords

Bacteremia; Blood culture; Diagnosis; Molecular; PCR; Sepsis

Indexed keywords

ANTIINFECTIVE AGENT;

ANTIBIOTIC RESISTANCE; ANTIMICROBIAL THERAPY; BACTEREMIA; BLOOD CULTURE; BLOODSTREAM INFECTION; CLINICAL PRACTICE; HUMAN; MASS SPECTROMETRY; NUCLEIC ACID AMPLIFICATION; NUCLEIC ACID HYBRIDIZATION; SEPTIC SHOCK; SHORT SURVEY;

BACTEREMIA; BLOOD CULTURE; DIAGNOSIS; MOLECULAR; PCR; SEPSIS;

ANTI-INFECTIVE AGENTS; BACTEREMIA; BACTERIOLOGICAL TECHNIQUES; HUMANS; MICROBIAL SENSITIVITY TESTS; SEPSIS;

EID: 84885387851     PISSN: 12019712     EISSN: 18783511     Source Type: Journal    
DOI: 10.1016/j.ijid.2013.04.018     Document Type: Short Survey

References (38)

1. Luzzaro F., Ortisi G., Larosa M., Drago M., Brigante G., Gesu G. Prevalence and epidemiology of microbial pathogens causing bloodstream infections: results of the OASIS multicenter study. Diagn Microbiol Infect Dis 2011, 69:363-369.
2. Byl B., Clevenbergh P., Jacobs F., Struelens M.J., Zech F., Kentos A., Thys J.P. Impact of infectious diseases specialists and microbiological data on the appropriateness of antimicrobial therapy for bacteremia. Clin Infect Dis 1999, 29:60-66. discussion 67-8.
3. Coburn B., Morris A.M., Tomlinson G., Detsky A.S. Does this adult patient with suspected bacteremia require blood cultures?. JAMA 2012, 308:502-511.
4. Shapiro N.I., Wolfe R.E., Wright S.B., Moore R., Bates D.W. Who needs a blood culture? A prospectively derived and validated prediction rule. J Emerg Med 2008, 35:255-264.
5. Jones G.R., Lowes J.A. The systemic inflammatory response syndrome as a predictor of bacteraemia and outcome from sepsis. QJM 1996, 89:515-522.
6. Hautala T., Syrjala H., Lehtinen V., Kauma H., Kauppila J., Kujala P., et al. Blood culture Gram stain and clinical categorization based empirical antimicrobial therapy of bloodstream infection. Int J Antimicrob Agents 2005, 25:329-333.
7. Skogberg K., Lyytikainen O., Ollgren J., Nuorti J.P., Ruutu P. Population-based burden of bloodstream infections in Finland. Clin Microbiol Infect 2012, 18:E170-E176.
8. Albrecht S.J., Fishman N.O., Kitchen J., Nachamkin I., Bilker W.B., Hoegg C., et al. Reemergence of Gram-negative health care-associated bloodstream infections. Arch Intern Med 2006, 166:1289-1294.
9. Bassetti M., Taramasso L., Nicco E., Molinari M.P., Mussap M., Viscoli C. Epidemiology, species distribution, antifungal susceptibility and outcome of nosocomial candidemia in a tertiary care hospital in Italy. PLoS One 2011, 6:e24198.
10. Kumar A., Ellis P., Arabi Y., Roberts D., Light B., Parillo J.E., et al. Initiation of inappropriate antimicrobial therapy results in a fivefold reduction of survival in human septic shock. Chest 2009, 136:1237-1248.
11. Ammerlaan H., Seifert H., Harbarth S., Brun-Buisson C., Torres A., Antonelli M., et al. Adequacy of antimicrobial treatment and outcome of Staphylococcus aureus bacteremia in 9 Western European countries. Clin Infect Dis 2009, 49:997-1005.
12. Rahikka P., Syrjanen J., Vuento R., Laine J., Huttunen R. Meticillin-resistant Staphylococcus aureus (MRSA) bacteraemia in Tampere University Hospital: a case-control study, Finland October 2002 to January 2010. Euro Surveill 2011, 16. pii: 19958.
13. Doern G.V., Vautour R., Gaudet M., Levy B. Clinical impact of rapid in vitro susceptibility testing and bacterial identification. J Clin Microbiol 1994, 32:1757-1762.
14. Barenfanger J., Drake C., Kacich G. Clinical and financial benefits of rapid bacterial identification and antimicrobial susceptibility testing. J Clin Microbiol 1999, 37:1415-1418.
15. Jorgensen J.H., Ferraro M.J. Antimicrobial susceptibility testing: a review of general principles and contemporary practices. Clin Infect Dis 2009, 49:1749-1755.
16. Paolucci M., Landini M.P., Sambri V. Conventional and molecular techniques for the early diagnosis of bacteraemia. Int J Antimicrob Agents 2010, 36(Suppl 2). S6-16.
17. Dark P., Wilson C., Blackwood B., McAuley D.F., Perkins G.D., MCMullan R., et al. Accuracy of LightCycler(R) SeptiFast for the detection and identification of pathogens in the blood of patients with suspected sepsis: a systematic review protocol. BMJ Open 2012, 2:e000392.
18. Mauro M.V., Cavalcanti P., Perugini D., Noto A., Sperli D., Giraldi C. Diagnostic utility of LightCycler SeptiFast and procalcitonin assays in the diagnosis of bloodstream infection in immunocompromised patients. Diagn Microbiol Infect Dis 2012, 73:308-311.
19. Pasqualini L., Mencacci A., Leli C., Montaqna P., Cardaccia A., Cenci E., et al. Diagnostic performance of a multiple real-time PCR assay in patients with suspected sepsis hospitalized in an internal medicine ward. J Clin Microbiol 2012, 50:1285-1288.
20. Yanagihara K., Kitagawa Y., Tomonaga M., Tsukasaki K., Kohno S., Seki M., et al. Evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection kit. Crit Care 2010, 14:R159.
21. Bravo D., Blanquer J., Tormo M., Aguilar G., Borras R., Solano C., et al. Diagnostic accuracy and potential clinical value of the LightCycler SeptiFast assay in the management of bloodstream infections occurring in neutropenic and critically ill patients. Int J Infect Dis 2011, 15:e326-e331.
22. Frye A.M., Baker C.A., Rustvold D.L., Heath K.A., Hunt J., Leggett J.E., Oethinger M. Clinical impact of a real-time PCR assay for rapid identification of staphylococcal bacteremia. J Clin Microbiol 2012, 50:127-133.
23. Karumaa S., Karpanoja P., Sarkkinen H. PCR identification of bacteria in blood culture does not fit the daily workflow of a routine microbiology laboratory. J Clin Microbiol 2012, 50:1031-1033.
24. Chuang Y.C., Chang S.C., Wang W.K. Using the rate of bacterial clearance determined by real-time polymerase chain reaction as a timely surrogate marker to evaluate the appropriateness of antibiotic usage in critical patients with Acinetobacter baumannii bacteremia. Crit Care Med 2012, 40:2273-2280.
25. Josefson P., Stralin K., Ohlin A., Ennefors T., Dragsten P., Andersson L., et al. Evaluation of a commercial multiplex PCR test (SeptiFast) in the etiological diagnosis of community-onset bloodstream infections. Eur J Clin Microbiol Infect Dis 2011, 30:1127-1134.
26. Funk D.J., Kumar A. Antimicrobial therapy for life-threatening infections: speed is life. Crit Care Clin 2011, 27:53-76.
27. Kumar A., Roberts D., Wood K.E., Light B., Parrillo J.E., Sharma S., et al. Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 2006, 34:1589-1596.
28. von Lilienfeld-Toal M., Lehmann L.E., Raadts A.D., Hahn-Ast C., Orlopp K.S., Marklein G., et al. Utility of a commercially available multiplex real-time PCR assay to detect bacterial and fungal pathogens in febrile neutropenia. J Clin Microbiol 2009, 47:2405-2410.
29. Shepard J.R., Addison R.M., Alexander B.D., Della-Latta P., Gherna M., Haase G., et al. Multicenter evaluation of the Candida albicans/Candida glabrata peptide nucleic acid fluorescent in situ hybridization method for simultaneous dual-color identification of C. albicans and C. glabrata directly from blood culture bottles. J Clin Microbiol 2008, 46:50-55.
30. Santolaya M.E., Farfan M.J., De La Maza V., Cociña M., Santelices F., Alvarez A.M., et al. Diagnosis of bacteremia in febrile neutropenic episodes in children with cancer: microbiologic and molecular approach. Pediatr Infect Dis J 2011, 30:957-961.
31. Lamoth F., Jaton K., Prod'hom G., Senn L., Bille J., Calandra T., Marchetti O. Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia. J Clin Microbiol 2010, 48:3510-3516.
32. Lau A., Halliday C., Chen S.C., Playford E.G., Stanley K., Sorrell T.C. Comparison of whole blood, serum, and plasma for early detection of candidemia by multiplex-tandem PCR. J Clin Microbiol 2010, 48:811-816.
33. Lehmann L.E., Alvarez J., Hunfeld K.P., Goglio A., Kost G.J., Louie R.F., et al. Potential clinical utility of polymerase chain reaction in microbiological testing for sepsis. Crit Care Med 2009, 37:3085-3090.
34. Louie R.F., Tang Z., Albertson T.E., Cohen S., Tran N.K., Kost G.J. Multiplex polymerase chain reaction detection enhancement of bacteremia and fungemia. Crit Care Med 2008, 36:1487-1492.
35. Lehmann L.E., Herpichboehm B., Kost G.J., Kollef M.H., Stüber F. Cost and mortality prediction using polymerase chain reaction pathogen detection in sepsis: evidence from three observational trials. Crit Care 2010, 14:R186.
36. Dierkes C., Ehrenstein B., Siebig S., Linde H.J., Reischl U., Salzberger B. Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis. BMC Infect Dis 2009, 9:126.
37. Pammi M., Flores A., Leeflang M., Versalovic J. Molecular assays in the diagnosis of neonatal sepsis: a systematic review and meta-analysis. Pediatrics 2011, 128:e973-e985.
38. Mancini N., Clerici D., Diotti R., Perotti M., Ghidoli N., De Marco D., et al. Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies. J Med Microbiol 2008, 57:601-604.